LOCUS AK222804 2280 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for threonyl-tRNA synthetase variant, clone:
HEP01768.
ACCESSION AK222804
VERSION AK222804.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2280)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2280
/cell_line="HepG2"
/clone="HEP01768"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000330584"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001444"
/organism="Homo sapiens"
/tissue_type="liver"
CDS <69..2240
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="threonyl-tRNA synthetase variant"
/protein_id="BAD96524.1"
/translation="MFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGR
AELNPWPEYIYTRLEMYNILKAEHDSILAEKAEKDSKPIKVTLPDGKQVDAESWKTTP
YQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHI
MGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFPSLEALCKKIIKEKQAFER
LEVKKETLLAMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALK
IHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYF
FHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHY
SENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALT
GLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGD
IEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQ
LPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVV
PVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKI
SGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF"
BASE COUNT 710 a 447 c 559 g 564 t
ORIGIN
1 acccgcctct tggctcctct cctctaggcc gtcgctttcg ggttctctca tcgcttcgtc
61 gttcgccaat gtttgaggag aaggccagca gtccttcagg gaagatggga ggcgaggaga
121 agccgattgg tgctggtgaa gagaagcaaa aggaaggagg caaaaagaag aacaaagaag
181 gatctggaga tggaggtcga gctgagttga atccttggcc tgaatatatt tacacacgtc
241 ttgagatgta taatatacta aaagcagagc atgattccat tctggcagaa aaggcagaaa
301 aagatagcaa gccaattaaa gtcactttgc ctgatggtaa acaggttgat gcggaatctt
361 ggaaaactac accatatcaa attgcctgtg gaattagtca aggcctggcc gacaacaccg
421 ttattgctaa agtaaataat gttgtgtggg acctggaccg ccctctggaa gaagattgta
481 ccttggagct tctcaagttt gaggatgagg aagctcaggc agtgtattgg cactctagtg
541 ctcacataat gggtgaagcc atggaaagag tctatggtgg atgtttatgc tacggtccgc
601 caatagaaaa tggattctat tatgacatgt acctcgaaga agggggtgtg tctagcaatg
661 atttcccttc tctggaggct ttgtgtaaga aaatcattaa agaaaaacaa gcttttgaaa
721 gactggaagt taagaaagaa actttactgg caatgtttaa gtacaacaag ttcaaatgcc
781 ggatattgaa tgaaaaggtg aatactccaa ctaccacagt ctatagatgt ggccctttga
841 tagatctctg ccggggtcct catgttagac acacgggcaa aattaaggct ttaaaaatac
901 acaaaaattc ctccacgtac tgggaaggca aagcagatat ggagactctc cagagaattt
961 atggcatttc attcccagat cctaaaatgt tgaaagagtg ggagaagttc caagaggaag
1021 ctaaaaaccg agatcatagg aaaattggca gggaccaaga actatatttc tttcatgaac
1081 tcagccctgg aagttgcttt tttctgccaa aaggagccta catttataat gcacttattg
1141 aattcattag gagcgaatat aggaaaagag gattccagga ggtagtcacc ccaaacatct
1201 tcaacagccg actctggatg acctcgggcc actggcagca ctacagcgag aacatgttct
1261 cctttgaggt ggagaaggag ctgtttgccc tgaaacccat gaactgccca ggacactgcc
1321 ttatgtttga tcatcggcca aggtcctggc gagaactgcc tctgcggcta gctgattttg
1381 gggtacttca taggaacgag ctgtctggag cactcacagg actcacccgg gtacgaagat
1441 tccaacagga tgatgctcac atattctgtg ccatggagca gattgaagat gaaataaaag
1501 gttgtttgga ttttctacgt acggtatata gcgtatttgg attttctttt aaactaaacc
1561 tttctactcg cccggaaaaa ttccttggag atatcgaagt atgggatcaa gctgagaaac
1621 aacttgaaaa cagtctgaat gaatttggtg aaaagtggga gttaaactct ggagatggag
1681 ctttctatgg cccaaagatt gacatacaga ttaaagatgc gattgggcgg taccaccagt
1741 gtgcaaccat ccagctggat ttccagttgc ccatcagatt taatcttact tatgtaagcc
1801 atgatggtga tgataagaaa aggccagtga ttgttcatcg agccatcttg ggatcagtgg
1861 aaagaatgat tgctatcctc acagaaaact atgggggcaa atggcccttt tggctgtccc
1921 ctcgccaggt aatggtagtt ccagtgggac caacctgtga tgaatatgcc caaaaggtac
1981 gacaacaatt ccacgatgcc aaattcatgg cagacattga tctggatcca ggctgtacat
2041 tgaataaaaa gattcgaaat gcacagttag cacagtataa cttcatttta gttgttggtg
2101 aaaaagagaa aatcagtggc actgttaata tccgcacaag agacaataag gtccacgggg
2161 aacgcaccat ttctgaaact atcgagcggc tacagcagct caaagagttc cgcagcaaac
2221 aggcagaaga agaattttaa tgaaaaaatt acccagattg gctccatgga aaaggaggaa
//