LOCUS AK222791 2508 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for elongator protein 2 variant, clone: HEP02825.
ACCESSION AK222791
VERSION AK222791.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2508)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2508
/cell_line="HepG2"
/clone="HEP02825"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000330571"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001393"
/organism="Homo sapiens"
/tissue_type="liver"
CDS <14..2494
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="elongator protein 2 variant"
/protein_id="BAD96511.1"
/translation="MVAPVLETSHVFCCPNRVRGVLNWSSGPRGLLAFGTSCSVVLYD
PLKRVVVTNLNGHTARVNCIQWICKQDGSPSTELVSGGSDNQVIHWEIEDNQLLKAVH
LQGHEGPVYAVHAVYQRRTSDPALCPLIVSAAADSAVRLWSKKGPEVMCLQTLNFGNG
FALALCLSFLPNTDVPILACGNDDCRIHIFAQQNDQFQKVLSLCGHEDWIRGVEWAAF
GRDLFLASCSQDCLIRIWKLYIKSTSLETQDDDNIRLKENTFTIENESVKIAFAVTLE
TVLAGHENWVNAVHWQPVFYKDGVLQQPMRLLSASMDKTMILWAPDEESGVWLEQVRV
GEVGGNTLGFYDCQFNEDGSMIIAHAFHGALHLWKQNTVNPREWTPEIVISGHFDGVQ
DLVWDPEGEFIITVGTDQTTRLFAPWKRKDQSQVTWHEIARPQIHGYDLKCLAMINRF
QFVSGADEKVLRVFSAPRNFVENFCAITGQSLNHVLCNQDSDLPEGATVPALGLSNKA
VFQGDIASQPSDEEELLTSTGFEYQQVAFQPSILTEPPTEDHLLQNTLWPEVQKLYGH
GYEIFCVTCNSSKTLLASACKAAKKEHAAIILWNTTSWKQVQNLVFHSLTVTQMAFSP
NEKFLLAVSRDRTWSLWKKQDTISPEFEPVFSLFAFTNKITSVHSRIIWSCDWSPDSK
YFFTGSRDKKVVVWGECDSTDDCIEHNIGPCSSVLDVGGAVTAVSVCPVLHPSQRYVV
AVGLECGKICLYTWKKTDQVPEINDWTHCVETSQSQSHTLAIRKLCWKNCSGKTEQKE
AEGAEWLHFASCGEDHTVKIHRVNKCAL"
BASE COUNT 697 a 490 c 604 g 717 t
ORIGIN
1 accagttggc gacatggtgg cacccgtgct ggagacttct cacgtgtttt gctgcccaaa
61 ccgggtgcgg ggagtcctga actggagctc tgggcccaga ggacttctgg cctttggcac
121 gtcctgctcc gtggtgctct atgaccccct gaaaagggtt gttgttacca acttgaatgg
181 tcacaccgcc cgagtcaatt gcatacagtg gatttgtaaa caggatggct ccccttctac
241 tgaattagtt tctggaggat ctgataatca agtgattcac tgggaaatag aggataatca
301 gcttttaaaa gcagtgcatc ttcaaggcca tgaaggacct gtttatgcgg tgcatgctgt
361 ttaccagagg aggacatcag atcctgcatt atgtccactg atcgtttctg cagctgcaga
421 ttctgctgtt cgactctggt ctaaaaaggg tccagaagta atgtgccttc agactttaaa
481 ctttggaaat ggatttgctt tggctctctg cttatctttt ttgccaaata ctgatgtacc
541 aatattagca tgtggcaatg atgattgcag aattcacata tttgctcaac aaaatgatca
601 gtttcagaaa gtgctttctc tctgtggaca tgaggattgg attagaggag tggaatgggc
661 agcctttggt agagatcttt tcctagcaag ctgttcacaa gattgcctga taagaatatg
721 gaagctgtat ataaagtcaa catctttaga aactcaggat gacgataaca taagactgaa
781 agaaaatact tttaccatag aaaatgaaag tgttaaaata gcatttgctg ttactctgga
841 gacagtgcta gccggtcatg aaaactgggt aaatgcagtt cactggcaac ctgtgtttta
901 caaagatggt gtcctacagc agccaatgag attattatct gcttccatgg ataaaaccat
961 gattctctgg gctccagatg aagagtcagg agtttggcta gaacaggttc gagtaggtga
1021 agtaggtggg aatactttgg gattttatga ttgccagttc aatgaagatg gttccatgat
1081 cattgctcat gctttccacg gagcgttgca cctttggaaa cagaatacag ttaacccaag
1141 agagtggact ccagagattg tcatttcagg acactttgat ggtgtccaag acctagtctg
1201 ggatccagaa ggagaattta ttatcactgt tggtactgat cagacaacta gactttttgc
1261 tccatggaag agaaaagacc aatcacaggt gacttggcat gaaattgcaa ggcctcagat
1321 acatgggtat gacctgaaat gtttggcaat gattaatcgg tttcagtttg tatctggagc
1381 agatgaaaaa gttcttcggg ttttttctgc acctcggaat tttgtggaaa atttttgtgc
1441 cattacagga caatcactga atcatgtgct ctgtaatcaa gatagtgatc ttccagaagg
1501 agccactgtc cctgcattgg gattatcaaa taaagctgtc tttcagggag atatagcttc
1561 tcagccttct gatgaagagg agctgttaac tagtactggt tttgagtatc agcaggtggc
1621 ctttcagccc tccatactta ctgagcctcc cactgaggat catcttctgc agaatacttt
1681 gtggcctgaa gttcaaaaac tatatgggca cggttatgaa atattttgtg ttacttgtaa
1741 cagttcaaag actctgcttg cctcagcttg taaggcagct aagaaagagc atgcagctat
1801 cattctttgg aacactacat cttggaaaca ggtgcagaat ttagttttcc acagtttgac
1861 agtcacgcag atggccttct cacctaatga gaagttctta ctagctgttt ccagagatcg
1921 aacctggtca ttgtggaaaa agcaggatac aatctcacct gagttcgagc cagtttttag
1981 tctttttgcc ttcaccaaca aaattacttc tgtgcacagt agaattattt ggtcttgtga
2041 ttggagtcct gacagcaagt atttcttcac tgggagtcga gacaaaaagg tggttgtctg
2101 gggtgagtgt gactccactg atgactgtat tgagcacaac attggcccct gctcctcagt
2161 cctggacgtg ggtggggctg tgacagctgt cagcgtctgc ccagtgctcc acccttctca
2221 acgatacgtg gttgcagtag gattggagtg tggaaagatt tgcttatata cctggaaaaa
2281 gactgatcaa gttccagaaa taaatgactg gacccactgt gtagaaacaa gtcaaagcca
2341 aagtcataca ctggctatca gaaaattatg ctggaagaat tgcagtggaa aaactgaaca
2401 gaaggaagca gaaggtgctg agtggttaca ctttgcaagc tgtggtgaag atcacactgt
2461 gaagatacac agagtcaata aatgtgcact gtaatggact taataact
//