LOCUS AK222786 2624 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for ribonucleoside-diphosphate reductase M1
chain variant, clone: HEP03925.
ACCESSION AK222786
VERSION AK222786.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2624)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2624
/cell_line="HepG2"
/clone="HEP03925"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000330566"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001374"
/organism="Homo sapiens"
/tissue_type="liver"
CDS <209..2587
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="ribonucleoside-diphosphate reductase M1 chain
variant"
/protein_id="BAD96506.1"
/translation="MHVIKRDGRQERVMFDKITSRIQKLCYGLNMDFVDPAQITMKVI
QGLYSGVTTVELDTLAAETAATLTTKHPDYAILAARIAVSNLHKETKKVFSDVMEDLY
NYINPHNGKHSPMVAKSTLDIVLANKDRLNSAIIYDRDFSYNYFGFKTLERSYLLKTN
GKVAERPQHMLMRVSVGIHKEDIDAAIETYNLLSERWFTHASPTLFNAGTNRPQLSSC
FLLSMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPML
RVYNNTARYVDQGGNKRPGAFAIYLEPWHLDIFEFLDLKKNTGKEEQRARDLFFALWI
PDLFMKRVETNQDWSLMCPNECPGLDEVWGEEFEKLYASYEKQGRVRKVVKAQQLWYA
IIESQTETGTPYMLYKDSCNRKSNQQNLGTIKCSNLCTEIVEYTSKDEVAVCNLASLA
LNMYVTSEHTYDFKKLAEVTKVVVRNLNKIIDINYYPVPEACLSNKRHRPIGIGVQGL
ADAFILMRYPFESAEAQLLNKQIFGTIYYGALEASCDLAKEQGPYETYEGSPVSKGIL
QYDMWNVTPTDLWDWKVLKEKIAKYGIRNSLLIAPMPTASTAQILGNNESIEPYTSNI
YTRRVLSGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKT
VWEISQKTVLKMAAERGAFIDQSQSLNIHVAEPNYGKLTSMHFYGWKQGLKTGMYYLR
TRPAANPIQFTLNKEKLKDKEKVSKEEEEKERNTAAMVCSLENRDECLMCGS"
BASE COUNT 764 a 552 c 620 g 688 t
ORIGIN
1 gtgcgtcacg ggtggcgggc gcgggaaggg gatttggatt gttgcgcctc tgctctgaag
61 aaagtgctgt ctggctccaa ctccagttct ttcccctgag cagcgcctgg aacctaaccc
121 ttcccactct gtcaccttct cgatcccgcc ggcgctttag agccgcagtc cagtcttgga
181 tccttcagag cctcagccac tagctgcgat gcatgtgatc aagcgagatg gccgccaaga
241 acgagtcatg tttgacaaaa ttacatctcg aatccagaag ctttgttatg gactcaatat
301 ggattttgtt gatcctgctc agatcaccat gaaagtaatc caaggcttgt acagtggggt
361 caccacagtg gaactagata ctttggctgc tgaaacagct gcaaccttga ctactaagca
421 ccctgactat gctatcctgg cagccaggat cgctgtctct aacttgcaca aagaaacaaa
481 gaaagtgttc agtgatgtga tggaagacct ctataactac ataaatccac ataatggcaa
541 acactctccc atggtggcca agtcaacatt ggatattgtt ctggccaata aagatcgcct
601 gaattctgct attatctatg accgagattt ctcttacaat tacttcggct ttaagacgct
661 agagcggtct tatttgttga agaccaatgg aaaagtggct gaaagaccac aacatatgtt
721 gatgagagta tctgttggga tccacaaaga agacattgat gcagcaattg aaacatataa
781 tcttctttct gagaggtggt ttactcatgc ttcgcccact ctcttcaatg ctggtaccaa
841 ccgcccacaa ctttctagct gttttcttct gagtatgaaa gatgacagca ttgaaggcat
901 ttatgacact ctaaagcaat gtgcattgat ttctaagtct gctggaggaa ttggtgttgc
961 tgtgagttgt attcgggcta ctggcagcta cattgctggg actaatggca attccaatgg
1021 ccttgtaccg atgctgagag tatataacaa cacagctaga tatgtggatc aaggtgggaa
1081 caagcgtcct ggggcatttg ctatttacct ggagccttgg catttagaca tctttgaatt
1141 ccttgattta aagaagaaca caggaaagga agagcagcgt gccagagatc ttttctttgc
1201 tctttggatt ccggatctct tcatgaaacg agtggagact aatcaggact ggtctttgat
1261 gtgtccaaat gagtgtcctg gtctggatga ggtttgggga gaggaatttg agaaactata
1321 tgcaagttat gagaaacaag gtcgtgtccg caaagttgta aaagctcagc agctttggta
1381 tgccatcatt gagtctcaga cggaaacagg caccccgtat atgctctaca aagattcctg
1441 taatcgaaag agcaaccagc agaacctggg aaccatcaaa tgcagcaacc tgtgcacaga
1501 aatagtggag tacaccagca aagatgaggt tgctgtttgt aatttggctt ccctggccct
1561 gaatatgtat gtcacatcag aacacacata cgactttaag aagttggctg aagtcactaa
1621 agtcgttgtc cgaaacttga ataaaattat tgatataaac tactatcctg taccagaggc
1681 atgcctatca aataaacgcc atcgccccat tggaattggg gtacaaggtc tggcagatgc
1741 ttttatcctg atgagatacc cttttgagag tgcagaagcc cagttactga ataagcagat
1801 ctttggaact atttattatg gtgctctgga agccagctgt gaccttgcca aggagcaggg
1861 cccatacgaa acctatgagg gctctccagt tagcaaagga attcttcagt atgatatgtg
1921 gaatgttact cctacagacc tatgggactg gaaggttctc aaggagaaga ttgcaaagta
1981 tggtataaga aacagtttac ttattgcccc gatgcctaca gcttccactg ctcagatcct
2041 ggggaataat gagtccattg aaccttacac cagcaacatc tatactcgca gagtcttgtc
2101 aggagaattt cagattgtaa atcctcactt attgaaagat cttaccgagc ggggcctatg
2161 gcatgaagag atgaaaaacc agattattgc atgcaatggc tctattcaga gcataccaga
2221 aattcctgat gacctgaagc aactttataa aactgtgtgg gaaatctctc agaaaactgt
2281 tctcaagatg gcagctgaga gaggtgcttt cattgatcaa agccaatctt tgaacatcca
2341 cgttgctgag cctaactatg gcaaactcac tagtatgcac ttctacggct ggaagcaggg
2401 tttgaagact gggatgtatt atttaaggac gagaccagca gctaatccaa tccagttcac
2461 tctaaataag gagaagctaa aagataaaga aaaggtatca aaagaggaag aagagaagga
2521 gaggaacaca gcagccatgg tgtgctcttt ggagaataga gatgaatgtc tgatgtgtgg
2581 atcctgagga aagacttgga agagaccagc atgtcttcag tagc
//