LOCUS AK222763 2206 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for galactosidase, beta 1 variant, clone:
HEP00366.
ACCESSION AK222763
VERSION AK222763.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2206)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2206
/cell_line="HepG2"
/clone="HEP00366"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000330543"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001001301"
/organism="Homo sapiens"
/tissue_type="liver"
CDS <37..2070
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="galactosidase, beta 1 variant"
/protein_id="BAD96483.1"
/translation="MPGFLVRILPLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKD
GQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDH
DVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDK
WLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLF
TTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDH
WGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYD
APLSEAGDLTEEYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDIL
CPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPFNGVHDRAYVAVDGI
PQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTD
WTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLP
QDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWA
PCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV"
BASE COUNT 513 a 592 c 559 g 542 t
ORIGIN
1 ctgggcgccg actgcagagc cgggaggctg gtggtcatgc cggggttcct ggttcgcatc
61 ctccctctgc tgctggttct gctgcttctg ggccctacgc gcggcttgcg caatgccacc
121 cagaggatgt ttgaaattga ctatagccgg gactccttcc tcaaggatgg ccagccattt
181 cgctacatct caggaagcat tcactactcc cgtgtgcccc gcttctactg gaaggaccgg
241 ctgctgaaga tgaagatggc tgggctgaac gccatccaga cgtatgtgcc ctggaacttt
301 catgagccct ggccaggaca gtaccagttt tctgaggacc atgatgtgga atattttctt
361 cggctggctc atgagctggg actgctggtt atcctgaggc ccgggcccta catctgtgca
421 gagtgggaaa tgggaggatt acctgcttgg ctgctagaga aagagtctat tcttctccgc
481 tcctccgacc cagattacct ggcagctgtg gacaagtggt tgggagtcct tctgcccaag
541 atgaagcctc tcctctatca gaatggaggg ccagttataa cagtgcaggt tgaaaatgaa
601 tatggcagct actttgcctg tgattttgac tacctgcgct tcctgcagaa gcgctttcgc
661 caccatctgg gggatgatgt ggttctgttt accactgatg gagcacataa aacattcctg
721 aaatgtgggg ccctgcaggg cctctacacc acggtggact ttggaacagg cagcaacatc
781 acagatgctt tcctaagcca gaggaagtgt gagcccaaag gacccttgat caattctgaa
841 ttctatactg gctggctaga tcactggggc caacctcact ccacaatcaa gaccgaagca
901 gtggcttcct ccctctatga tatacttgcc cgtggggcga gtgtgaactt gtacatgttt
961 ataggtggga ccaattttgc ctattggaat ggggccaact caccctatgc agcacagccc
1021 accagctacg actatgatgc cccactgagt gaggctgggg acctcactga ggagtatttt
1081 gctctgcgaa acatcatcca gaagtttgaa aaagtaccag aaggtcctat ccctccatct
1141 acaccaaagt ttgcatatgg aaaggtcact ttggaaaagt taaagacagt gggagcagct
1201 ctggacattc tgtgtccctc tgggcccatc aaaagccttt atcccttgac atttatccag
1261 gtgaaacagc attatgggtt tgtgctgtac cggacaacac ttcctcaaga ttgcagcaac
1321 ccagcacctc tctcttcacc cttcaatgga gtccacgatc gagcatatgt tgctgtggat
1381 gggatccccc agggagtcct tgagcgaaac aatgtgatca ctctgaacat aacagggaaa
1441 gctggagcca ctctggacct tctggtagag aacatgggac gtgtgaacta tggtgcatat
1501 atcaacgatt ttaagggttt ggtttctaac ctgactctca gttccaatat cctcacggac
1561 tggacgatct ttccactgga cactgaggat gcagtgcgca gccacctggg gggctgggga
1621 caccgtgaca gtggccacca tgatgaagcc tgggcccaca actcatccaa ctacacgctc
1681 ccggcctttt atatggggaa cttctccatt cccagtggga tcccagactt gccccaggac
1741 acctttatcc agtttcctgg atggaccaag ggccaggtct ggattaatgg ctttaacctt
1801 ggccgctatt ggccagcccg gggccctcag ttgaccttgt ttgtgcccca gcacatcctg
1861 atgacctcgg ccccaaacac catcaccgtg ctggaactgg agtgggcacc ctgcagcagt
1921 gatgatccag aactatgtgc tgtgacgttc gtggacaggc cagttattgg ctcatctgtg
1981 acctacgatc atccctccaa acctgttgaa aaaagactca tgcccccacc cccgcaaaaa
2041 aacaaagatt catggctgga ccatgtatga tgatgaaagc ctgtgtcttt gagggattct
2101 accctgaaca tacctcacag atcctccctg tcatgccaca tttcactgat tggaatgtgg
2161 aaatggaaaa ggaatttagg atgtgcattt tcacctgagg tttccc
//