LOCUS AK222614 2478 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for cysteine-tRNA ligase isoform a variant,
clone: CAS12492.
ACCESSION AK222614
VERSION AK222614.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2478)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2478
/cell_type="primary smooth muscle cells of human coronary
artery"
/clone="CAS12492"
/clone_lib="CAS"
/db_xref="H-InvDB:HIT000330394"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001000527"
/organism="Homo sapiens"
/tissue_type="coronary artery"
CDS <26..2206
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="cysteine-tRNA ligase isoform a variant"
/protein_id="BAD96334.1"
/translation="MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFMP
QDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITDIDDKI
IKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQ
LATEPLEKAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKF
WEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNSVQKIVDNGYGYVSNGSVYFDTAKFA
SSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWP
CPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVR
YFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTME
SALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDN
VDTRTVMEEMRALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSS
LGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPE
LGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVEEEKRKKKEEAARRKQEQEAAKLAK
MKIPPSEMFLSETDKYSKFDENVSMVCPHMTWRAKSSAKGKPRS"
BASE COUNT 671 a 599 c 681 g 527 t
ORIGIN
1 agctgcgggt ccgggattcc cagccatggc agattcctcc gggcagcagg gcaaaggccg
61 gcgtgtgcag ccccagtggt cccctcctgc tgggacccag ccatgcagac tccaccttta
121 caacagcctc accaggaaca aggaagtgtt catgcctcaa gatgggaaaa aggtgacgtg
181 gtattgctgt gggccaaccg tctatgacgc atctcacatg gggcacgcca ggtcctacat
241 ctcttttgat atcttgagaa gagtgttgaa ggattacttc aaatttgatg tcttttattg
301 catgaacatt acggatattg atgacaagat catcaagagg gcccggcaga accacctgtt
361 cgagcagtat cgggagaaga ggcctgaagc ggcacagctc ttggaggatg ttcaggccgc
421 cctgaagcca ttttcagtaa aattaaatga gaccacggat cccgataaaa agcagatgct
481 cgaacggatt cagcacgcag tgcagcttgc cacagagcca cttgagaaag ctgtgcagtc
541 cagactcacg ggagaggaag tcaacagctg tgtggaggtg ttgctggaag aagccaagga
601 tttgctctct gactggctgg attctacact tggctgtgat gtcactgaca attccatctt
661 ctccaagctg cccaagttct gggaggggga cttccacaga gacatggaag ctctgaatgt
721 tctccctcca gatgtcttaa cccgggttag tgagtatgtg ccagaaattg tgaactctgt
781 ccagaagatt gtggacaacg gttacggcta tgtctccaat gggtctgtct actttgatac
841 agcgaagttt gcttctagcg agaagcactc ctatgggaag ctggtgcctg aggccgttgg
901 agatcagaaa gcccttcaag aaggggaagg tgacctgagc atctctgcag accgcctgag
961 tgagaagcgc tctcccaacg actttgcctt atggaaggcc tctaagcccg gagaaccgtc
1021 ctggccgtgc ccttggggaa agggtcgtcc gggctggcat atcgagtgct cggccatggc
1081 aggcaccctc ctaggggctt cgatggacat tcacggaggt gggttcgacc tccggttccc
1141 ccaccatgac aatgagctgg cacagtcgga ggcctacttt gaaaacgact gctgggtcag
1201 gtacttcctg cacacaggcc acctgaccat tgcaggctgc aaaatgtcaa agtcactaaa
1261 aaacttcatc accattaaag atgccttgaa aaagcactca gcacggcagt tgcggctggc
1321 cttcctcatg cactcgtgga aggacaccct ggactactcc agcaacacca tggagtcagc
1381 gcttcaatat gagaagttct tgaatgagtt tttcttaaat gtgaaagata tccttcgcgc
1441 tcctgttgac atcactggtc agtttgagaa gtggggagaa gaagaagcag aactgaataa
1501 gaacttttat gacaagaaga cagcaattca caaagccctc tgtgacaatg ttgacacccg
1561 caccgtcatg gaagagatgc gggccttggt cagtcagtgc aacctctata tggcagcccg
1621 gaaagccgtg aggaagaggc ccaaccaggc tctgctggag aacatcgccc tgtacctcac
1681 ccatatgctg aagatctttg gggccgtaga agaggacagc tccctgggat tcccggtcgg
1741 agggcctgga accagcctca gtctcgaggc cacagtcatg ccctaccttc aggtgttatc
1801 agaattccga gaaggagtgc ggaagattgc ccgagagcag aaagtccctg agattctgca
1861 gctcagcgat gccctgcggg acaacatcct gcctgagctt ggggtgcggt ttgaagacca
1921 cgaaggactg cccacagtgg tgaaactggt agacagaaac accttattaa aagagagaga
1981 agaaaagaga cgggttgaag aggagaagag gaagaagaaa gaggaggcgg cccggaggaa
2041 acaggaacaa gaagcagcaa agctggccaa gatgaagatt ccccccagtg agatgttctt
2101 gtcagaaacc gacaaatact ccaagtttga tgaaaatgta agcatggtct gcccacacat
2161 gacatggagg gcaaagagct cagcaaaggg caagccaaga agctgaagaa gctcttcgag
2221 gctcaggaga agctctacaa ggaatatcag cagatggccc agaatggaag cttccagtga
2281 gggggcacag gactgacttt ttaaaccatt gtggactagt ggctgctgtc tgcctcagtg
2341 acaatgtccc agcgctccta tcatgtttac agtcaccctt gggtcctaaa ttaagagttg
2401 tgttcatgta ggttcgtgtc gtcgttggct ctgagacatt gataataaat ttttctcaac
2461 agtgaaaaaa aaaaaaaa
//