LOCUS AK222586 3060 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for minichromosome maintenance protein 3
variant, clone: CAS04981.
ACCESSION AK222586
VERSION AK222586.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3060)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..3060
/cell_type="primary smooth muscle cells of human coronary
artery"
/clone="CAS04981"
/clone_lib="CAS"
/db_xref="H-InvDB:HIT000330366"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001000405"
/organism="Homo sapiens"
/tissue_type="coronary artery"
CDS <46..2472
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="minichromosome maintenance protein 3 variant"
/protein_id="BAD96306.1"
/translation="MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQ
YRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGL
EGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYS
DLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVD
VILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSF
SAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSH
IRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERR
LEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLA
AANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYR
APGEQNGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEEMVSAA
FMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRL
ATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQ
EDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELS
ESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQV
MVSEGIIFLI"
BASE COUNT 803 a 736 c 830 g 691 t
ORIGIN
1 gaacctccac gcgactttgg tggaggtagt tctttggcag cgggcatggc gggtaccgtg
61 gtgctggacg atgtggagct gcgggaggct cagagagatt acctggactt cctggacgac
121 gaggaagacc agggaattta tcagagcaaa gttcgggagc tgatcagtga caaccaatac
181 cggctgattg tcaatgtgaa tgacctgcgc aggaaaaacg agaagagggc taaccggctt
241 ctgaacaatg cctttgagga gctggttgcc ttccagcggg ccttaaagga ttttgtggcc
301 tccattgatg ctacctatgc caagcagtat gaggagttct acgtaggact ggaaggcagc
361 tttggctcca agcacgtctc cccgcggact cttacctcct gcttcctcag ctgtgtggtc
421 tgtgtggaag gcattgtcac taaatgttct ctagttcgtc ccaaagtcgt ccgcagtgtc
481 cactactgtc ctgctactaa gaagaccata gagcgacgtt attctgatct caccaccctg
541 gtggcctttc cctccagctc tgtctatcct accaaggatg aggagaacaa tccccttgag
601 acagaatatg gcctttctgt ctacaaggat caccagacca tcaccatcca ggagatgccg
661 gagaaggccc cagccggcca gctcccccgc tctgtggacg tcattctgga tgatgacttg
721 gtggataaag cgaagcctgg tgaccgggtt caggtggtgg gaacctaccg ttgccttcct
781 ggaaagaagg gaggctacac ctctgggacc ttcaggactg tcctgattgc ctgtaatgtt
841 aagcagatga gcaaggatgc tcagccctct ttctctgctg aggatatagc caagatcaag
901 aagttcagta aaacccgatc caaggatatc tttgaccagc tggccaagtc attggcccca
961 agtatccatg ggcatgacta tgtcaagaaa gcaatcctct gcttgctctt gggaggggtg
1021 gaacgagacc tagaaaatgg cagccacatc cgtggagaca tcaatattct tctaatagga
1081 gacccatccg ttgccaagtc tcagcttctg cggtatgtgc tttgcactgc accccgagct
1141 atccccacca ctggccgggg ctcctctgga gtgggtctga cggctgctgt caccacagac
1201 caggaaacag gagagcgccg tctggaagca ggggccatgg tcctggctga ccgaggcgtg
1261 gtttgcattg atgaatttga caaaatgtct gacatggatc gcacagccat ccatgaagtg
1321 atggagcagg gtcgagtgac cattgccaag gctggcatcc atgctcggct gaatgcccgc
1381 tgcagtgttt tggcagctgc caaccctgtc tacggcaggt atgaccagta taagactcca
1441 atggagaaca ttgggctaca ggactcactg ctgtcacgat ttgacttgct cttcatcatg
1501 ctggatcaga tggatcctga gcaggatcgg gagatctcag accatgtcct tcggatgcac
1561 cgttacagag cacctgggga gcagaatggc gatgctatgc ccttgggtag tgctgtggat
1621 atcctggcca cagatgatcc caactttagc caggaagatc agcaggacac ccagatttat
1681 gagaagcatg acaaccttct acatgggacc aagaagaaaa aggaggagat ggtgagtgca
1741 gcattcatga agaagtacat ccatgtggcc aaaatcatca agcctgtcct gacacaggag
1801 tcggccacct acattgcaga agagtattca cgcctgcgca gccaggatag catgagctca
1861 gacaccgcca ggacatctcc agttacagcc cgaacactgg aaactctgat tcgactggcc
1921 acagcccatg cgaaggcccg catgagcaag actgtggacc tgcaggatgc agaggaagct
1981 gtggagttgg tccagtatgc ttactttaag aaggttctgg agaaggagaa gaaacgtaag
2041 aagcgaagtg aggatgaatc agagacagaa gatgaagagg agaaaagcca agaggaccag
2101 gagcagaaga ggaagagaag gaagactcgc cagccagatg ccaaagatgg ggattcatac
2161 gacccctatg acttcagtga cacagaggag gaaatgcctc aagtacacac tccaaagacg
2221 gcagactcac aggagaccaa ggaatcccag aaagtggagt tgagtgaatc caggttgaag
2281 gcattcaagg tggccctctt ggatgtgttc cgggaagctc atgcgcagtc aatcggcatg
2341 aatcgcctca cagaatccat caaccgggac agcgaagagc ccttctcttc agttgagatc
2401 caggctgctc tgagtaagat gcaggatgac aatcaggtca tggtgtctga gggcatcatc
2461 ttcctcatct gaggaggcct cgtctctgaa cttgggttgt gccgagagag tttgttctgt
2521 gtttcccacc ctctccctga cccaagtctt tgcctctact cccttaacag tgttgaattc
2581 aactgaaggc gaggaatgtt ggtgatgaag ctgagttcag gactcggtgg accctttggg
2641 aatgggtcat gaaagctgcc atggggtgag gaaagaggag acagtgggag aggacaatga
2701 ctattgcatc ttcattgcaa gagcaccggc tcatccgccc tacttcccat cccacacaaa
2761 cccaattgta aataacatat gacttctgag tacttttggg ggcacaactg ttttctgttt
2821 gctgtttttt tgttttgttt tttttctcca gagcactttg gtctagacta ggctttgggt
2881 ggttccaatt ggtggagaga agctctgagg cacgtcatgc aggtcaagaa agctttcttt
2941 gcagtagcac cagttaaggt gaatatgtat tgtatcacaa aacaaaccca atatccagat
3001 gaatatccga gatgttgaat aaacttagcc atttcgtaca caaaaaaaaa aaaaaaaaaa
//