LOCUS AK222522 2144 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for granulin variant, clone: adSE02012.
ACCESSION AK222522
VERSION AK222522.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2144)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2144
/clone="adSE02012"
/clone_lib="ad"
/db_xref="H-InvDB:HIT000330302"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001000171"
/organism="Homo sapiens"
/tissue_type="adipose tissue"
CDS <41..1822
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="granulin variant"
/protein_id="BAD96242.1"
/translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRP
LLDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRG
FHCSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCED
RVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDG
STCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPA
HTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGT
CEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIP
EAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEEMPARRASLSHPRDIGCDQHTSC
PVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFL
ARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCA
ARGTKCLRREAPRWDAPLRDPALRQLL"
BASE COUNT 386 a 689 c 640 g 429 t
ORIGIN
1 gctgctgccc aaggaccgcg gagtcggacg caggcagacc atgtggaccc tggtgagctg
61 ggtggcctta acagcagggc tggtggctgg aacgcggtgc ccagatggtc agttctgccc
121 tgtggcctgc tgcctggacc ccggaggagc cagctacagc tgctgccgtc cccttctgga
181 caaatggccc acaacactga gcaggcatct gggtggcccc tgccaggttg atgcccactg
241 ctctgccggc cactcctgca tctttaccgt ctcagggact tccagttgct gccccttccc
301 agaggccgtg gcatgcgggg atggccatca ctgctgccca cggggcttcc actgcagtgc
361 agacgggcga tcctgcttcc aaagatcagg taacaactcc gtgggtgcca tccagtgccc
421 tgatagtcag ttcgaatgcc cggacttctc cacgtgctgt gttatggtcg atggctcctg
481 ggggtgctgc cccatgcccc aggcttcctg ctgtgaagac agggtgcact gctgtccgca
541 cggtgccttc tgcgacctgg ttcacacccg ctgcatcaca cccacgggca cccaccccct
601 ggcaaagaag ctccctgccc agaggactaa cagggcagtg gccttgtcca gctcggtcat
661 gtgtccggac gcacggtccc ggtgccctga tggttctacc tgctgtgagc tgcccagtgg
721 gaagtatggc tgctgcccaa tgcccaacgc cacctgctgc tccgatcacc tgcactgctg
781 cccccaagac actgtgtgtg acctgatcca gagtaagtgc ctctccaagg agaacgctac
841 cacggacctc ctcactaagc tgcctgcgca cacagtgggg gatgtgaaat gtgacatgga
901 ggtgagctgc ccagatggct atacctgctg ccgtctacag tcgggggcct ggggctgctg
961 cccttttacc caggctgtgt gctgtgagga ccacatacac tgctgtcccg cggggtttac
1021 gtgtgacacg cagaagggta cctgtgaaca ggggccccac caggtgccct ggatggagaa
1081 ggccccagct cacctcagcc tgccagaccc acaagccttg aagagagatg tcccctgtga
1141 taatgtcagc agctgtccct cctccgatac ctgctgccaa ctcacgtctg gggagtgggg
1201 ctgctgtcca atcccagagg ctgtctgctg ctcggaccac cagcactgct gcccccaggg
1261 ctacacgtgt gtagctgagg ggcagtgtca gcgaggaagc gagatcgtgg ctggactgga
1321 ggagatgcct gcccgccggg cttccttatc ccaccccaga gacatcggct gtgaccagca
1381 caccagctgc ccggtggggc agacctgctg cccaagcctg ggtgggagct gggcctgctg
1441 ccagttgccc catgctgtgt gctgcgagga tcgccagcac tgctgcccgg ctggctacac
1501 ctgcaacgtg aaggctcgat cctgcgagaa ggaagtggtc tctgcccagc ctgccacctt
1561 cctggcccgt agccctcacg tgggtgtgaa ggacgtggag tgtggggaag gacacttctg
1621 ccatgataac cagacctgct gccgagacaa ccgacagggc tgggcctgct gtccctaccg
1681 ccagggcgtc tgttgtgctg atcggcgcca ctgctgtcct gctggcttcc gctgcgcagc
1741 caggggtacc aagtgtttgc gcagggaggc cccgcgctgg gacgcccctt tgagggaccc
1801 agccttgaga cagctgctgt gagggacagt actgaagact ctgcagccct cgggacccca
1861 ctcggagggt gccctctgct cgggcctccc tagcacctct ccctaaccaa attctccctg
1921 gaccccattc tgagctcccc atcaccatgg gaggtggggc ctcaatctaa ggccttccct
1981 gtcagaaggg ggttgtggca aaagccacat tacaagctgc catcccctcc ccgtttcagt
2041 ggaccctgtg gccaggtgct tttccctatc cacaggggtg tttgtgtgtg tgcgcgtgtg
2101 cgtttcaata aagtttgtac actttaaaaa aaaaaaaaaa aaaa
//