LOCUS AK222486 2367 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for aldehyde dehydrogenase 4A1 precursor
variant, clone: adKA03036.
ACCESSION AK222486
VERSION AK222486.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2367)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eucaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 3
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library.
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..2367
/clone="adKA03036"
/clone_lib="ad"
/db_xref="H-InvDB:HIT000330266"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001000070"
/organism="Homo sapiens"
/tissue_type="adipose tissue"
CDS <33..1724
/codon_start=1
/inference="non-experimental evidence, no additional
details recorded"
/note="Start codon is not identified."
/product="aldehyde dehydrogenase 4A1 precursor variant"
/protein_id="BAD96206.1"
/translation="MLLPAPALRRALLSRPWTGAGLRWKHTSSLKVANEPVLAFTQGS
PERDALQKALKDLKGRMEAIPCVVGDEEVWTSDVQYQVSPFNHGHKVAKFCYADKSLL
NKAIEAALAARKEWDLKPIADRAQIFLKAADMLSGPRRAEILAKTMVGQGKTVIQAEI
DAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGLEGFVAAISPFNFTAIGGN
LAGAPALIGNVVLWKPSDTAMLASYAVYRILREAGLPPNIIQFVPADGPLFGDTVTSS
EHLCGINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVS
GTLRSAFEYGGQKCSACSRLYVPHSLWPQIKGRLLEEHSRIRVGDPAEDFGTFFSAVI
DAKSFARIKKWLEHARSSPSLTILAGGKCDDSVGYFVEPCIVESKDPQEPIMKEEIFG
PVLSVYVYPDDKYKETLQLVDSTTSYGLTGAVFSQDKDVVQEATKVLRNAAGNFYIND
KSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYAYMQ"
BASE COUNT 449 a 749 c 706 g 463 t
ORIGIN
1 gttccagcga acagccccgc ttctaacccg agatgctgct gccggcgccc gcgctccgcc
61 gcgccctgct gtcccgcccc tggaccgggg ccggcctgcg gtggaagcac acctcctccc
121 tgaaggtggc caacgagccc gtcttagcct tcacgcaggg cagccctgag cgagatgccc
181 tgcaaaaggc cttgaaggac ctgaagggcc ggatggaagc catcccatgc gtggtggggg
241 atgaggaggt gtggacgtcg gacgtgcagt accaagtgtc gccttttaac catggacata
301 aggtggccaa gttctgttat gcagacaaga gcctgctcaa caaagccatt gaggctgccc
361 tggctgcccg gaaagagtgg gacctgaagc ctattgcaga ccgggcccag atcttcctga
421 aggcggcaga catgctgagt gggccgcgca gggctgagat cctcgccaag accatggtgg
481 gacagggtaa gaccgtgatc caagcggaga ttgacgctgc agcggaactc atcgacttct
541 tccggttcaa tgccaagtat gcggtggagc tggaggggca gcagcccatc agcgtgcccc
601 cgagcaccaa cagcacggtg taccggggtc tggagggctt cgtggcggcc atctcgccct
661 ttaacttcac tgcaatcggc ggcaacctgg cgggggcacc ggccctgata ggcaacgtgg
721 tcctatggaa gcccagtgac actgccatgc tggccagcta tgctgtctac cgcatccttc
781 gggaggctgg cctgcccccc aacatcatcc agtttgtgcc agctgatggg cccctatttg
841 gggacactgt caccagctca gagcacctct gtggcatcaa cttcacaggc agtgtgccca
901 ccttcaaaca cctgtggaag caggtggccc agaacctgga ccggttccac accttcccac
961 gcctggctgg agagtgcggc ggaaagaact tccacttcgt gcaccgctcg gccgacgtgg
1021 agagcgtggt gagcgggacc ctccgctcag ccttcgagta cggtggccag aagtgttccg
1081 cctgctcgcg tctctacgtg ccgcactcgc tgtggccgca gatcaaaggg cggctgctgg
1141 aggagcacag tcggatcaga gtgggcgacc ctgcagagga ttttgggacc ttcttctctg
1201 cagtgattga tgccaagtcc tttgcccgta tcaagaagtg gctggagcac gcacgctcct
1261 cacccagcct caccatcctg gccgggggca agtgtgatga ctccgtgggc tactttgtgg
1321 agccctgcat cgtggagagc aaggaccctc aggagcccat catgaaggag gagatcttcg
1381 ggcctgtact gtctgtgtac gtctacccgg atgacaagta caaggagacg ctgcagctgg
1441 ttgacagcac caccagctat ggcctcacgg gggcagtgtt ctcccaggat aaggacgtcg
1501 tgcaggaggc cacaaaggtg ctgaggaatg ctgccggcaa cttctacatc aacgacaagt
1561 ccactggctc gatagtgggc cagcagccct ttgggggggc ccgagcctct ggaaccaatg
1621 acaagccagg gggcccacac tacatcctgc gctggacgtc gccgcaggtc atcaaggaga
1681 cacataagcc cctgggggac tggagctacg cgtacatgca gtgagcccct ctcgggctcc
1741 accgtccagc tgtctgtccg tccaggtggc cgacctcact gcacagaccc cactccagcc
1801 cctccacccc ttcttcatgc acagctgcct ttctataatc cgggcttgac tcccttctta
1861 ccactgtatt ctggcctctc ccatgcctca ggctctggtt tgagatcgtg ctggggagga
1921 acatggccac taccccttat ctcatcggcc atgtgggagg tatgaccctg gtgcctggca
1981 ggttctccct ctgccctcca ctgggcccag tggctcaggg acctggggaa aggagatgga
2041 gcagctcttg ggatcctttg gggaaaagga ggccattctg ggccccttgg caaacctcac
2101 cactcacaga ggctcctggc cttgatccct gcccctccag gtgtccaggg taaagtgtaa
2161 ctcagactga cctgtggggc acagggggca ccagctggcc ttgccctctc tggtctgggc
2221 tgtctacctt cctcactgta tctttgccca gacccacctg ggccagtagg cccctgtccc
2281 cagccacaca ccttagatgc tggcatgcct tactccaggt gcctgtgttt ggccgaggcc
2341 tgtgtgattc ccggtctgca ccacatg
//