LOCUS AK207914 404 bp mRNA linear HTC 19-SEP-2008 DEFINITION Mus musculus cDNA, clone:Y2G0109I08, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027766, based on BLAT search. ACCESSION AK207914 VERSION AK207914.1 KEYWORDS HTC; ASSETS. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 404) AUTHORS Arakawa,T., Carninci,P., Fukuda,S., Harbers,M., Hayatsu,N., Hori,F., Imotani,K., Kawai,J., Kondo,S., Murata,M., Nakamura,M., Nomura,K., Ohno,M., Sasaki,D., Shiraki,T., Waki,K., Watahiki,A. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (15-SEP-2004) to the DDBJ/EMBL/GenBank databases. Contact:Yoshihide Hayashizaki The Institute of Physical and Chemical Research (RIKEN), Omics Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan URL :http://www.osc.riken.jp/ REFERENCE 2 AUTHORS Watahiki,A., Waki,K., Hayatsu,N., Shiraki,T., Kondo,S., Nakamura,M., Sasaki,D., Arakawa,T., Kawai,J., Harbers,M., Hayashizaki,Y. and Carninci,P. TITLE Libraries enriched for alternatively spliced exons reveal splicing patterns in melanocytes and melanomas JOURNAL Nat. Methods 1, 233-239 (2004) COMMENT Alternative Splicing Libraries (ASLs) are prepared by: Preparating of single-stranded DNA using a RNA template from full length cDNA libraries, hybridizating of single-stranded DNAs, removing of remaining single-stranded DNA, digesting of regions comprising double-stranded DNA by a set of 4 bp-cutters, capturing of DNA hybrids with loop structures (alternative spliced exon), ligating of Y-shaped primers to isolated DNA hybrids with loop structures, PCR amplification of ligation products and their cloning into pFLCI vector. (Reference) FEATURES Location/Qualifiers source 1..404 /cell_line="mixture of B16-F10Y and melan-c" /cell_type="mixture of melanoma cell and melanocyte cell" /clone="Y2G0109I08" /clone_lib="Alternative Splicing Library L3" /db_xref="taxon:10090" /mol_type="mRNA" /note="strand:minus, reference:ENSEMBL:Mouse-Transcript- ENST:ENSMUST00000027766, based on BLAT search" /organism="Mus musculus" BASE COUNT 94 a 109 c 110 g 91 t ORIGIN 1 tgccatctcc tccctgctgt ggatgtacaa ccaggaacag gagcagtgca gtactaaact 61 caacacagcc acctttgggg ggtctagttg gccatgggaa tgaattgtgt gtttctgggt 121 gagcttatgt ccctgccctt tgccacccca gcctgtggcc tggagctaga agctcagggc 181 agcagggtag ccaccctagc tcagtctcgg catggacaac agaccctttt aaaagcaggg 241 ctgacactgg atgagcagca gggctccagc atgccccagg agaggtagct atcaccccac 301 cccaggggag cgacacaggc tctttgtttg tatgtcttgt aattacacat tcataccttt 361 gcgttcaata aagaagtgcg ttggggacta ccgaaaacgg tccg //