LOCUS       AK196494                 258 bp    mRNA    linear   HTC 19-SEP-2008
DEFINITION  Mus musculus cDNA, clone:Y1G0122A08, strand:minus,
            reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000050211, based
            on BLAT search.
ACCESSION   AK196494
VERSION     AK196494.1
KEYWORDS    HTC; ASSETS.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Arakawa,T., Carninci,P., Fukuda,S., Harbers,M., Hayatsu,N.,
            Hori,F., Imotani,K., Kawai,J., Kondo,S., Murata,M., Nakamura,M.,
            Nomura,K., Ohno,M., Sasaki,D., Shiraki,T., Waki,K., Watahiki,A.
            and Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2004) to the DDBJ/EMBL/GenBank databases.
            Contact:Yoshihide Hayashizaki
            The Institute of Physical and Chemical Research (RIKEN), Omics
            Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
            Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
            URL    :http://www.osc.riken.jp/
REFERENCE   2
  AUTHORS   Watahiki,A., Waki,K., Hayatsu,N., Shiraki,T., Kondo,S.,
            Nakamura,M., Sasaki,D., Arakawa,T., Kawai,J., Harbers,M.,
            Hayashizaki,Y. and Carninci,P.
  TITLE     Libraries enriched for alternatively spliced exons reveal splicing
            patterns in melanocytes and melanomas
  JOURNAL   Nat. Methods 1, 233-239 (2004)
COMMENT     Alternative Splicing Libraries (ASLs) are prepared by:
            Preparating of single-stranded DNA using a RNA template
            from full length cDNA libraries, hybridizating of
            single-stranded DNAs, removing of remaining single-stranded
            DNA, digesting of regions comprising double-stranded DNA
            by a set of 4 bp-cutters, capturing of DNA hybrids with loop
            structures (alternative spliced exon), ligating of Y-shaped
            primers to isolated DNA hybrids with loop structures, PCR
            amplification of ligation products and their cloning into
            pFLCI vector. (Reference)
FEATURES             Location/Qualifiers
     source          1..258
                     /cell_line="mixture of B16-F10Y and melan-c"
                     /cell_type="mixture of melanoma cell and melanocyte cell"
                     /clone="Y1G0122A08"
                     /clone_lib="Alternative Splicing Library L1"
                     /db_xref="taxon:10090"
                     /mol_type="mRNA"
                     /note="strand:minus,
                     reference:ENSEMBL:Mouse-Transcript-
                     ENST:ENSMUST00000050211, based on BLAT search"
                     /organism="Mus musculus"
BASE COUNT           65 a           57 c           69 g           67 t
ORIGIN      
        1 cgtgcagctc gccatgctgg accagcacac gcgccctcag atgtcacctg tacggttgat
       61 ggaaaggtca atttacagcg caagatacat ttttgtagga aacctgtata gaagtgggaa
      121 gatgcccgag gttgactacg cgattctgtc tgagtggttt gactggatcg tcaggaacat
      181 tgatgtctct gttgatctga tagtttatct gcgaaccact cccgaaatct gctaccagag
      241 attaaagatg aggtgccg
//