LOCUS       AK194514                 375 bp    mRNA    linear   HTC 19-SEP-2008
DEFINITION  Mus musculus cDNA, clone:Y1G0115I24, strand:plus,
            reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000036928, based
            on BLAT search.
ACCESSION   AK194514
VERSION     AK194514.1
KEYWORDS    HTC; ASSETS.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Arakawa,T., Carninci,P., Fukuda,S., Harbers,M., Hayatsu,N.,
            Hori,F., Imotani,K., Kawai,J., Kondo,S., Murata,M., Nakamura,M.,
            Nomura,K., Ohno,M., Sasaki,D., Shiraki,T., Waki,K., Watahiki,A.
            and Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2004) to the DDBJ/EMBL/GenBank databases.
            Contact:Yoshihide Hayashizaki
            The Institute of Physical and Chemical Research (RIKEN), Omics
            Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
            Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
            URL    :http://www.osc.riken.jp/
REFERENCE   2
  AUTHORS   Watahiki,A., Waki,K., Hayatsu,N., Shiraki,T., Kondo,S.,
            Nakamura,M., Sasaki,D., Arakawa,T., Kawai,J., Harbers,M.,
            Hayashizaki,Y. and Carninci,P.
  TITLE     Libraries enriched for alternatively spliced exons reveal splicing
            patterns in melanocytes and melanomas
  JOURNAL   Nat. Methods 1, 233-239 (2004)
COMMENT     Alternative Splicing Libraries (ASLs) are prepared by:
            Preparating of single-stranded DNA using a RNA template
            from full length cDNA libraries, hybridizating of
            single-stranded DNAs, removing of remaining single-stranded
            DNA, digesting of regions comprising double-stranded DNA
            by a set of 4 bp-cutters, capturing of DNA hybrids with loop
            structures (alternative spliced exon), ligating of Y-shaped
            primers to isolated DNA hybrids with loop structures, PCR
            amplification of ligation products and their cloning into
            pFLCI vector. (Reference)
FEATURES             Location/Qualifiers
     source          1..375
                     /cell_line="mixture of B16-F10Y and melan-c"
                     /cell_type="mixture of melanoma cell and melanocyte cell"
                     /clone="Y1G0115I24"
                     /clone_lib="Alternative Splicing Library L1"
                     /db_xref="taxon:10090"
                     /mol_type="mRNA"
                     /note="strand:plus,
                     reference:ENSEMBL:Mouse-Transcript-
                     ENST:ENSMUST00000036928, based on BLAT search"
                     /organism="Mus musculus"
BASE COUNT           88 a           99 c           98 g           90 t
ORIGIN      
        1 cggacactgt gggctgtgct ctgaatgact ctcctgtggg cctggctgcc tacatcttag
       61 agaagttctc cacctggacc aagtcagaat accgtgaact ggaggatgga ggcctggaga
      121 ggaagttctc cctggaagat ctgctgacta acatcatgat ctactggacg acaggaacca
      181 ttgtctcctc ccagcgcctt ctacaaggaa aacttgggcc agggtgtcat ggtccataga
      241 catgagggga tgaaggtctt tgtgccactg gctattcagc cttcccttct gagatcctgc
      301 atgccccaga aaagtgggtg aaggtcaagt accccaaact catctcctat tcctaaatgg
      361 aacggcctct ctggt
//