LOCUS AK187139 248 bp mRNA linear HTC 19-SEP-2008 DEFINITION Mus musculus cDNA, clone:Y0G0139P10, strand:minus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027802, based on BLAT search. ACCESSION AK187139 VERSION AK187139.1 KEYWORDS HTC; ASSETS. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 248) AUTHORS Arakawa,T., Carninci,P., Fukuda,S., Harbers,M., Hayatsu,N., Hori,F., Imotani,K., Kawai,J., Kondo,S., Murata,M., Nakamura,M., Nomura,K., Ohno,M., Sasaki,D., Shiraki,T., Waki,K., Watahiki,A. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (15-SEP-2004) to the DDBJ/EMBL/GenBank databases. Contact:Yoshihide Hayashizaki The Institute of Physical and Chemical Research (RIKEN), Omics Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan URL :http://www.osc.riken.jp/ REFERENCE 2 AUTHORS Watahiki,A., Waki,K., Hayatsu,N., Shiraki,T., Kondo,S., Nakamura,M., Sasaki,D., Arakawa,T., Kawai,J., Harbers,M., Hayashizaki,Y. and Carninci,P. TITLE Libraries enriched for alternatively spliced exons reveal splicing patterns in melanocytes and melanomas JOURNAL Nat. Methods 1, 233-239 (2004) COMMENT Alternative Splicing Libraries (ASLs) are prepared by: Preparating of single-stranded DNA using a RNA template from full length cDNA libraries, hybridizating of single-stranded DNAs, removing of remaining single-stranded DNA, digesting of regions comprising double-stranded DNA by a set of 4 bp-cutters, capturing of DNA hybrids with loop structures (alternative spliced exon), ligating of Y-shaped primers to isolated DNA hybrids with loop structures, PCR amplification of ligation products and their cloning into pFLCI vector. (Reference) FEATURES Location/Qualifiers source 1..248 /cell_line="mixture of B16-F10Y and melan-c" /cell_type="mixture of melanoma cell and melanocyte cell" /clone="Y0G0139P10" /clone_lib="Alternative Splicing Library L2" /db_xref="taxon:10090" /mol_type="mRNA" /note="strand:minus, reference:ENSEMBL:Mouse-Transcript- ENST:ENSMUST00000027802, based on BLAT search" /organism="Mus musculus" BASE COUNT 62 a 43 c 72 g 71 t ORIGIN 1 cggaagtctg tggagagctt tagttagatc cgagggctgc ttcagattga tctccaggtg 61 aggtgagagg aaacgggaat cctctgaatt aaaaacacgc aaagaagcct gtgtcttggt 121 gttgcaggtg gaagtgtggg gagtcatcct gtgacctctt gcttaactct tctggacagg 181 tgacccatta gaaggtgatg ttcagggctg ctttaataaa gatcttattt atttttgaag 241 acagtccg //