LOCUS       AK187139                 248 bp    mRNA    linear   HTC 19-SEP-2008
DEFINITION  Mus musculus cDNA, clone:Y0G0139P10, strand:minus,
            reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027802, based
            on BLAT search.
ACCESSION   AK187139
VERSION     AK187139.1
KEYWORDS    HTC; ASSETS.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Arakawa,T., Carninci,P., Fukuda,S., Harbers,M., Hayatsu,N.,
            Hori,F., Imotani,K., Kawai,J., Kondo,S., Murata,M., Nakamura,M.,
            Nomura,K., Ohno,M., Sasaki,D., Shiraki,T., Waki,K., Watahiki,A.
            and Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2004) to the DDBJ/EMBL/GenBank databases.
            Contact:Yoshihide Hayashizaki
            The Institute of Physical and Chemical Research (RIKEN), Omics
            Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
            Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
            URL    :http://www.osc.riken.jp/
REFERENCE   2
  AUTHORS   Watahiki,A., Waki,K., Hayatsu,N., Shiraki,T., Kondo,S.,
            Nakamura,M., Sasaki,D., Arakawa,T., Kawai,J., Harbers,M.,
            Hayashizaki,Y. and Carninci,P.
  TITLE     Libraries enriched for alternatively spliced exons reveal splicing
            patterns in melanocytes and melanomas
  JOURNAL   Nat. Methods 1, 233-239 (2004)
COMMENT     Alternative Splicing Libraries (ASLs) are prepared by:
            Preparating of single-stranded DNA using a RNA template
            from full length cDNA libraries, hybridizating of
            single-stranded DNAs, removing of remaining single-stranded
            DNA, digesting of regions comprising double-stranded DNA
            by a set of 4 bp-cutters, capturing of DNA hybrids with loop
            structures (alternative spliced exon), ligating of Y-shaped
            primers to isolated DNA hybrids with loop structures, PCR
            amplification of ligation products and their cloning into
            pFLCI vector. (Reference)
FEATURES             Location/Qualifiers
     source          1..248
                     /cell_line="mixture of B16-F10Y and melan-c"
                     /cell_type="mixture of melanoma cell and melanocyte cell"
                     /clone="Y0G0139P10"
                     /clone_lib="Alternative Splicing Library L2"
                     /db_xref="taxon:10090"
                     /mol_type="mRNA"
                     /note="strand:minus,
                     reference:ENSEMBL:Mouse-Transcript-
                     ENST:ENSMUST00000027802, based on BLAT search"
                     /organism="Mus musculus"
BASE COUNT           62 a           43 c           72 g           71 t
ORIGIN      
        1 cggaagtctg tggagagctt tagttagatc cgagggctgc ttcagattga tctccaggtg
       61 aggtgagagg aaacgggaat cctctgaatt aaaaacacgc aaagaagcct gtgtcttggt
      121 gttgcaggtg gaagtgtggg gagtcatcct gtgacctctt gcttaactct tctggacagg
      181 tgacccatta gaaggtgatg ttcagggctg ctttaataaa gatcttattt atttttgaag
      241 acagtccg
//