LOCUS AK154160 2381 bp mRNA linear HTC 06-OCT-2010
DEFINITION Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN
full-length enriched library, clone:F630005E19 product:thymidine
kinase 2, mitochondrial, full insert sequence.
ACCESSION AK154160
VERSION AK154160.1
KEYWORDS HTC_FLI; HTC; CAP trapper.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 2381)
AUTHORS Arakawa,T., Carninci,P., Fukuda,S., Hashizume,W., Hayashida,K.,
Hori,F., Iida,J., Imamura,K., Imotani,K., Itoh,M., Kanagawa,S.,
Kawai,J., Kojima,M., Konno,H., Murata,M., Nakamura,M.,
Ninomiya,N., Nishiyori,H., Nomura,K., Ohno,M., Sakazume,N.,
Sano,H., Sasaki,D., Shibata,K., Shiraki,T., Tagami,M., Tagami,Y.,
Waki,K., Watahiki,A., Muramatsu,M. and Hayashizaki,Y.
TITLE Direct Submission
JOURNAL Submitted (30-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Yoshihide Hayashizaki
The Institute of Physical and Chemical Research (RIKEN), Omics
Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
URL :http://www.osc.riken.jp/
REFERENCE 2
AUTHORS
CONSRTM The FANTOM Consortium, Riken Genome Exploration Research Group and
Genome Science Group (Genome Network Project Core Group)
TITLE The Transcriptional Landscape of the Mammalian Genome
JOURNAL Science 309, 1559-1563 (2005)
REFERENCE 3
AUTHORS
CONSRTM RIKEN Genome Exploration Research Group and Genome Science Group
(Genome Network Project Core Group) and the FANTOM Consortium
TITLE Antisense Transcription in the Mammalian Transcriptome
JOURNAL Science 309, 1564-1566 (2005)
REFERENCE 4
AUTHORS
CONSRTM The FANTOM Consortium and the RIKEN Genome Exploration Research
Group Phase I and II Team
TITLE Analysis of the mouse transcriptome based on functional annotation
of 60,770 full-length cDNAs
JOURNAL Nature 420, 563-573 (2002)
REFERENCE 5
AUTHORS
CONSRTM The RIKEN Genome Exploration Research Group Phase II Team and the
FANTOM Consortium
TITLE Functional annotation of a full-length mouse cDNA collection
JOURNAL Nature 409, 685-690 (2001)
REFERENCE 6
AUTHORS Carninci,P. and Hayashizaki,Y.
TITLE High-efficiency full-length cDNA cloning
JOURNAL Meth. Enzymol. 303, 19-44 (1999)
REFERENCE 7
AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K.,
Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
TITLE Normalization and subtraction of cap-trapper-selected cDNAs to
prepare full-length cDNA libraries for rapid discovery of new genes
JOURNAL Genome Res. 10, 1617-1630 (2000)
REFERENCE 8
AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N.,
Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai,T.,
Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M.,
Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S.,
Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y.,
Izawa,M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K.,
Tanaka,T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M.,
Inoue,Y., Kira,A. and Hayashizaki,Y.
TITLE RIKEN integrated sequence analysis (RISA) system-384-format
sequencing pipeline with 384 multicapillary sequencer
JOURNAL Genome Res. 10, 1757-1771 (2000)
COMMENT cDNA library was prepared and sequenced in Mouse Genome
Encyclopedia Project of Genome Exploration Research Group in Riken
Genomic Sciences Center and Genome Science Laboratory in RIKEN.
Division of Experimental Animal Research in Riken contributed to
prepare mouse tissues.
Tissues were provided by Dr. John Todd (Dept. of Medical Genetics
Wellcome Trust Centre for Molecular Mechanisms in Disease Wellcome
Trust/MRC building Addenbrookes Hospital Cambridge) whose
assistance we gratefully acknowledge.
Please visit our web site for further details.
URL:http://www.osc.riken.jp/
URL:http://fantom.gsc.riken.jp/
clone information is available at:
http://fantom.gsc.riken.jp/3/db/annotate/
main.cgi?masterid=F630005E19
FEATURES Location/Qualifiers
source 1..2381
/cell_type="NOD-derived CD11c +ve dendritic cells"
/clone="F630005E19"
/clone_lib="RIKEN full-length enriched mouse cDNA library"
/db_xref="FANTOM_DB:F630005E19"
/db_xref="MGI:3552816"
/db_xref="taxon:10090"
/mol_type="mRNA"
/organism="Mus musculus"
/strain="NOD"
CDS 20..832
/codon_start=1
/note="putative"
/note="thymidine kinase 2, mitochondrial (MGD|MGI:1913266
GB|AK087557, evidence: BLASTN, 99%, match=2380)"
/protein_id="BAE32414.1"
/transl_table=1
/translation="MLLRSLRSWAARSLRSVGPGSSGSPGSLDSGAGPLWAPRRAWPP
DKDRENDKEKKAVVCIEGNIASGKTTCLEFFSNTTDVEVLMEPVLKWRNVHGHNPLSL
MYHDASRWGLTLQTYVQLTMLDQHTRPQMSPVRLMERSIYSARYIFVENLYRSGKMPE
VDYAILSEWFDWIVRNIDVSVDLIVYLRTTPEICYQRLKMRCREEEKVIPMEYLHAIH
RLYEEWLVNGSLFPAAAPVLVIEADHNLEKMLELFEQNRARILTPENWKHGP"
regulatory 2366..2371
/note="putative"
/regulatory_class="polyA_signal_sequence"
polyA_site 2381
/note="putative"
BASE COUNT 557 a 595 c 650 g 579 t
ORIGIN
1 ggcacatcag ccagggacta tgctgctgcg gtcgctgcgt agttgggctg cccggtccct
61 gcgtagcgtg ggcccgggga gctccgggag ccccgggagc ctcgattcgg gcgccgggcc
121 gctgtgggca cctcgccgcg cctggcctcc ggataaagat agagaaaatg ataaggagaa
181 aaaagcagtg gtttgtattg agggcaatat tgcaagtggg aagacgacat gcctggagtt
241 cttctccaat acaacagacg tcgaggtgtt aatggagcct gtgctcaagt ggagaaatgt
301 ccatggccat aaccctctga gcctcatgta ccatgatgcc agccgatggg gcctcacact
361 gcagacgtac gtgcagctca ccatgctgga ccagcacacg cgccctcaga tgtcacctgt
421 acggttgatg gaaaggtcaa tttacagcgc aagatacatt tttgtagaaa acctgtatag
481 aagtgggaag atgcccgagg ttgactacgc gattctgtct gagtggtttg actggatcgt
541 caggaacatt gatgtctctg ttgatctgat agtttatctg cgaaccactc ccgaaatctg
601 ctaccagaga ttaaagatga ggtgccggga agaggagaaa gtcattccga tggaatacct
661 ccatgctatt caccgcctct acgaggagtg gctggtcaac gggagcctct tcccagctgc
721 agcccctgtt ctggtgattg aggctgacca caacttggag aaaatgttag aactctttga
781 acaaaaccgg gcccggatat taactccaga gaactggaag catggaccct aggactgagt
841 gatctacaag acagcgccag gaacagccca gccagggcca ggttagctgc taggagcatt
901 tggaagaatc tactcttagg agggtctgtt atctaatcag atctattttc tcaatggtct
961 tttcactgca gtacaaatgg cttttgtctg tcagatgctt gcagcataca tccctcctgg
1021 tgcccctcct ggtggcttcc taacgatgaa gccctgtgtt aaaatcactg actgtatccc
1081 tggggttgtt ctggatcctc tgcatcaggt cacctcatgg gtgagggagc agtcttccac
1141 cccaggcaag gatttgaagc ccagagaggt gtggggactc gtcaagccag agcattgtgg
1201 agggagctgg gaccattcag atacctggtt tgggctgcct cctcctaagc tgttccccac
1261 cgccctgctg ctctgagcac cagcccccgc ctctgtgaga agagtggaca gtttgtgaat
1321 tccatcatct ttcagtaccg ggccagttca aggagaccag acatcactag gtgttggcac
1381 acctctctta tttggcatga gcccagggtg tcctggtatg agggtactgt gacctctatc
1441 cattcctttg tcacctggac ctcggtgtcc actggggact cctggatcgg cctctgcttg
1501 gtgccagagt ccttgaggat gagtcctccc cagactctac cagttccctg tcgttttgtg
1561 tcagaaaaaa gggggttatg ggacctggac actttaccct ttgctgagcg aggccaaggg
1621 cagccagctg agcttccaag accccatcac tctctcctgt ccttgtgtga ggagcagtgt
1681 ccccttgctc cagggagagt gacagtggcc ttaactcctt tgaagtgttt ttccatctga
1741 gcagatgtca tggaccacga gcatgaagaa gtcatctcta ttggatcaga aaatacctta
1801 gctttgctga gccgtaactg tgcactgaag gtgcagctgt tacttgaatc agtcatgacc
1861 aagagaggcc ggccattcct caggaccgtg agactggaaa gtcatggttt gccaacaaag
1921 acaatttaag ccttgctgct cttgacattc tagaatggat ggagtgcatc tggaaaagac
1981 tggacacaga tgatacaggg gaatgatgac cgtataccct ggagaagcac agcacaggct
2041 ggggaggggg ctgcagggga gccctgctcc cctgtttagc tgcctgggag ctagaaagag
2101 ggccaagagt ccagctcttg gtcctttctg tttggaccaa ttcttggccc ttttggtagt
2161 atgaagagta gcatccaggg agattgtcca tcagcacctg cagtccagca atggataaac
2221 ctgagaaagg caggaacttt gaaccagcag tactcatggg ggcatcttta gaatttgcaa
2281 acgaacagtc tagtacttgc cctttgctct accagaacac ttgttttggg gagaaatgtg
2341 ctgtttctct ttctctctag tagccaataa aattctacag c
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