LOCUS AK136575 1973 bp mRNA linear HTC 06-OCT-2010
DEFINITION Mus musculus adult male cecum cDNA, RIKEN full-length enriched
library, clone:9130409I23 product:similar to Putative fatty acid
desaturase MLD (DEGENERATIVE spermatocyte) (Homolog DROSOPHILA,
lipid desaturase) (Sphingolipid delta 4 desaturase protein DES1)
[Homo sapiens], full insert sequence.
ACCESSION AK136575
VERSION AK136575.1
KEYWORDS HTC_FLI; HTC; CAP trapper.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 1973)
AUTHORS Arakawa,T., Carninci,P., Fukuda,S., Hashizume,W., Hayashida,K.,
Hori,F., Iida,J., Imamura,K., Imotani,K., Itoh,M., Kanagawa,S.,
Kawai,J., Kojima,M., Konno,H., Murata,M., Nakamura,M.,
Ninomiya,N., Nishiyori,H., Nomura,K., Ohno,M., Sakazume,N.,
Sano,H., Sasaki,D., Shibata,K., Shiraki,T., Tagami,M., Tagami,Y.,
Waki,K., Watahiki,A., Muramatsu,M. and Hayashizaki,Y.
TITLE Direct Submission
JOURNAL Submitted (30-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Yoshihide Hayashizaki
The Institute of Physical and Chemical Research (RIKEN), Omics
Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
URL :http://www.osc.riken.jp/
REFERENCE 2
AUTHORS
CONSRTM The FANTOM Consortium, Riken Genome Exploration Research Group and
Genome Science Group (Genome Network Project Core Group)
TITLE The Transcriptional Landscape of the Mammalian Genome
JOURNAL Science 309, 1559-1563 (2005)
REFERENCE 3
AUTHORS
CONSRTM RIKEN Genome Exploration Research Group and Genome Science Group
(Genome Network Project Core Group) and the FANTOM Consortium
TITLE Antisense Transcription in the Mammalian Transcriptome
JOURNAL Science 309, 1564-1566 (2005)
REFERENCE 4
AUTHORS
CONSRTM The FANTOM Consortium and the RIKEN Genome Exploration Research
Group Phase I and II Team
TITLE Analysis of the mouse transcriptome based on functional annotation
of 60,770 full-length cDNAs
JOURNAL Nature 420, 563-573 (2002)
REFERENCE 5
AUTHORS
CONSRTM The RIKEN Genome Exploration Research Group Phase II Team and the
FANTOM Consortium
TITLE Functional annotation of a full-length mouse cDNA collection
JOURNAL Nature 409, 685-690 (2001)
REFERENCE 6
AUTHORS Carninci,P. and Hayashizaki,Y.
TITLE High-efficiency full-length cDNA cloning
JOURNAL Meth. Enzymol. 303, 19-44 (1999)
REFERENCE 7
AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K.,
Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
TITLE Normalization and subtraction of cap-trapper-selected cDNAs to
prepare full-length cDNA libraries for rapid discovery of new genes
JOURNAL Genome Res. 10, 1617-1630 (2000)
REFERENCE 8
AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N.,
Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai,T.,
Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M.,
Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S.,
Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y.,
Izawa,M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K.,
Tanaka,T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M.,
Inoue,Y., Kira,A. and Hayashizaki,Y.
TITLE RIKEN integrated sequence analysis (RISA) system-384-format
sequencing pipeline with 384 multicapillary sequencer
JOURNAL Genome Res. 10, 1757-1771 (2000)
COMMENT cDNA library was prepared and sequenced in Mouse Genome
Encyclopedia Project of Genome Exploration Research Group in Riken
Genomic Sciences Center and Genome Science Laboratory in RIKEN.
Division of Experimental Animal Research in Riken contributed to
prepare mouse tissues.
Please visit our web site for further details.
URL:http://www.osc.riken.jp/
URL:http://fantom.gsc.riken.jp/
clone information is available at:
http://fantom.gsc.riken.jp/3/db/annotate/
main.cgi?masterid=9130409I23
FEATURES Location/Qualifiers
source 1..1973
/clone="9130409I23"
/clone_lib="RIKEN full-length enriched mouse cDNA library"
/db_xref="FANTOM_DB:9130409I23"
/db_xref="MGI:3539589"
/db_xref="taxon:10090"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Mus musculus"
/sex="male"
/strain="C57BL/6J"
/tissue_type="cecum"
CDS <1..1027
/codon_start=2
/note="putative"
/note="similar to Putative fatty acid desaturase MLD
(DEGENERATIVE spermatocyte) (Homolog DROSOPHILA, lipid
desaturase) (Sphingolipid delta 4 desaturase protein
DES1) [Homo sapiens] (UniProt|O15121, evidence: FASTY,
74.5%ID, 97.2%length, match=942)"
/note="start codon is not identified"
/protein_id="BAE23057.1"
/transl_table=1
/translation="DPVTLSELHLEQILGQVCTPAMRKLASLEDAEWVYNDRLHNDRR
REILAKYPEIKSLMKPDPNLIWIITIIILIQLALLYLVKDLEWKWVIFCAYVFGGSIN
HSLIIASHEISHNFPFGHRKALWNRWFGIFANLPIGIPCSISFKKYHTDHHRFLGVDG
VDVGVPHYLEGWFFCTAYRKLVWIAFQPLFITFRLLFINRKPISYLEIINTVVQITFD
IIVYYVFGVKSLVYMLVASMLGLGLHPISGNLIGDHYMFLKDQETFSYYGPLNLLTFN
VGYHNEHHDFPNIPGKDLPLVRKIAAEYYDKLPHHISWIKVLYDFVMDNTISSYSRMK
KRLPKRK"
BASE COUNT 579 a 395 c 398 g 601 t
ORIGIN
1 ggatcctgtc accctctcgg agttgcacct ggagcagatc ctagggcagg tgtgcacacc
61 agccatgagg aaactcgcat cactggaaga tgctgagtgg gtgtacaatg atcggctgca
121 taatgatcga cgtcgggaga tattggcaaa atatccagag ataaaatctt tgatgaaacc
181 ggaccctaac ctgatctgga tcataaccat aataattctt atccagctgg ctttgttgta
241 cttagtcaaa gacttggagt ggaaatgggt catattttgt gcctatgtat ttggtggttc
301 tattaaccac tcactaatta tagcttctca tgagatctct cataatttcc cctttggcca
361 ccgcaaagca ctgtggaacc gctggtttgg aatatttgct aacctcccga ttgggattcc
421 atgctcgatt tcctttaaga aataccacac ggaccaccat cgattccttg gagttgatgg
481 tgtcgacgta ggtgttcctc actatctcga gggctggttc ttctgcaccg cttacaggaa
541 gcttgtctgg attgcttttc aacctctctt tattactttt cgacttctat ttatcaaccg
601 caaaccaatt tcttatctgg aaatcatcaa tactgttgtt cagatcacct ttgacattat
661 agtttactat gtctttgggg ttaaatcctt agtctacatg ctggtagcct ccatgcttgg
721 tctgggtttg cacccaataa gtggcaatct tataggggac cattacatgt tcttaaagga
781 ccaggaaaca ttctcctatt atgggcctct gaacttgctc accttcaatg tgggctatca
841 caacgagcac catgacttcc ccaacattcc tggaaaagac cttcccttgg tgaggaaaat
901 agcagctgaa tactatgaca aactccccca tcacatctcc tggatcaaag tactgtatga
961 ttttgtgatg gataacacaa taagttccta ttcacggatg aagaagaggc ttccaaaaag
1021 gaaatagctt ttagagcaag cactgtcaga gtcaaagcaa agtctgcaaa gatttttaat
1081 tgctgataat gtggattttt ttttcttgtt aaaattaaga gctgttagaa gccatctagg
1141 aaaggctatg gcaagcaagt gagttttctg gtgatgaccc actgttttgc agggctgtaa
1201 cagatgtact ctgagatgca aggtcctcag acatttcatc ccaggattgc ttcttgctac
1261 caatatgcct ttcctgccac tcttacatag cctaatgatt cctgggcatc tgccaactat
1321 attgggacga tttcctgcag aatcaatatg aagatggtac tttcatgtag ttatgctgtg
1381 tagatgaact gatgacttca tatttctgtt tatagaactc ctaaattgat ataggtactc
1441 tcaagccccc tatagaacaa acgctgcaaa tagagctctg taaaccttca tgtgtcatgg
1501 gataattgca ttagataaag aaaccaggct tggatcaaat tccttctagg tcagaaatga
1561 agccactatc tggtaactag gggccataaa ctgggaatgg gaaattgatt gtaggtgaaa
1621 ggacaaaaca taaatgatgg actaaaacca gtcttagcca agatttcaaa atagtaagac
1681 acaaggcaga tgatttgtct cttgacaaat tctgtgctaa cttatgacat aaaaattatt
1741 gctttaaact tttaatgagc ttatggatct agcaatagta gataatggat gtttagtcag
1801 gtactagcct aatggaaaga catgtaaatg attctgttgt aattccttaa gccttattgc
1861 tctatgaatt tatggaatgt aaaaatgctt agaaaatcat gtgattaatt ttcctgagaa
1921 agtataaaaa ctaagaacaa caataaaatg gtagtgcaaa ctttttcagc ttc
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