LOCUS AK131412 3961 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ16518 fis, clone NT2RI3007443, moderately
similar to MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5 (EC
2.7.1.-).
ACCESSION AK131412
VERSION AK131412.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3961)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Oshima,A., Takahashi-Fujii,A., Tanase,T., Imose,N., Takeuchi,K.,
Arita,M., Musashino,K., Yuuki,H., Hara,H., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2004)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..3961
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3007443"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000249700"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 1024..3270
/codon_start=1
/protein_id="BAD18559.1"
/translation="MKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFST
EEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVY
AGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIF
MEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNT
YSGVVKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTI
IEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATT
AELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSSSEHGSVSPDSDA
QPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAIL
YKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISK
LKVDLDFDSSSISQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTIL
IPELRAHFEPTCETEGVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNR
LLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDW
LRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQE
ASETKDKA"
BASE COUNT 1119 a 919 c 991 g 932 t
ORIGIN
1 atgacatccg gaaagccaga gagaaatacc aaggtgagga actggcgaag gagctagctc
61 ggatcaagct ccgcatggat aatactgagg ttctgacctc agacatcatc attaacttac
121 tcctgtccta ccgtgatatc caggactatg atgcgatggt gaagctggtg gaaacactgg
181 agatgctgcc tacgtgtgat ttggccgatc agcataacat taaattccac tatgcgtttg
241 cactgaatag gagaaacagc acaggtgacc gtgagaaggc tctgcagatc atgctccagg
301 ttctgcagag ctgtgatcac ccgggccccg acatgttctg cctgtgtggg aggatctaca
361 aggacatctt cttggattca gactgcaaag atgacaccag ccgcgacagc gccattgagt
421 ggtatcgcaa agggtttgaa ctccagtcat ccctctattc gggaattaat cttgcagttt
481 tgctgattgt tgctggacaa caatttgaaa cttccttgga actaaggaaa ataggtgtcc
541 ggctgaacag tttgttggga agaaaaggga gcttggagaa aatgaacaat tactgggatg
601 tgggtcagtt cttcagcgtc agcatgctgg cccatgatgt cgggaaagcc gtccaggcag
661 cagagaggtt gttcaaactg aaacctccag tctggtacct gcgatcatta gttcagaact
721 tgttactaat tcggcgcttc aagaaaacca ttattgaaca ctcgcccagg caagagcggc
781 tgaacttctg gttagatata atttttgagg caacaaatga agtcactaat ggactcagat
841 ttccattatg aaatatttca aacctactga aaggtacaaa aaaataatgg agcagacaac
901 cgcgtaatct ctaaccaggt tctggtcata gagccaacca aagtgtacca gccttcttat
961 gtttccataa acaatgaagc cgaggagaga acagtttctt tatggcatgt ctcacccaca
1021 gaaatgaaac agatgcacga atggaatttt acagcctctt ccataaaggg aataagccta
1081 tcaaagtttg atgaaaggtg ttgttttctt tatgtccatg ataattctga tgactttcaa
1141 atctactttt ccaccgaaga gcagtgcagt agatttttct ctttggtcaa agagatgata
1201 accaatacag caggcagtac ggtggagctg gagggagaga ccgatggaga caccttggag
1261 tatgagtatg accatgatgc aaatggtgag agagttgtct tggggaaagg cacgtatggg
1321 attgtgtatg ctggccgaga tctgagcaat caagtgcgaa tagccatcaa agaaatcccg
1381 gagagagata gcaggtattc tcagcctctg cacgaggaga tagccctgca caagtacctt
1441 aagcaccgca atatcgttca gtacctgggc tctgtttcag agaacggcta cattaagata
1501 tttatggagc aggtgcctgg aggaagcctt tctgctcttc tgcgatccaa atgggggccg
1561 atgaaggaac cgacaatcaa gttttacacc aaacagatcc tggagggcct taagtatctt
1621 catgaaaacc agatcgtgca cagagacata aagggcgata atgttctggt gaacacctac
1681 agcggagtgg tgaaaatctc cgattttgga acctcgaaac gtcttgcggg tgtgaacccc
1741 tgcacagaga cttttactgg caccctgcag tacatggcac ctgagataat tgaccaaggg
1801 cctcgcggat atggtgcccc agccgatatc tggtccctgg gctgcaccat cattgagatg
1861 gccaccagca agcctccgtt ccatgagctt ggtgagccgc aggcagccat gttcaaagtg
1921 ggcatgttta agatccaccc tgagattcca gaagcccttt cagctgaagc ccgagccttc
1981 attttatcct gtttcgagcc tgacccccac aaacgtgcca ccactgctga gctactgaga
2041 gagggtttct taaggcaggt gaacaagggc aagaagaacc gaattgcctt caagccctca
2101 gaaggtcccc gcggtgtcgt cctggccctg cccacacagg gagagcccat ggccaccagc
2161 agcagcgagc acggctctgt ctccccagac tccgacgccc agcctgacgc actctttgag
2221 aggacccggg cgcccaggca ccaccttggc cacctcctca gtgttccaga cgagagctca
2281 gccttggaag accggggctt ggcctcgtcc ccggaggaca gggaccaggg cctcttcctg
2341 ctacgcaagg acagtgagcg ccgtgccatc ctgtacaaaa tcctctggga ggagcagaac
2401 caggtggctt ccaacctgca ggagtgtgtg gcccagagtt ccgaagagtt gcatctctca
2461 gttggacaca tcaagcaaat cattgggatc ctgagggact tcatccgctc cccagagcac
2521 cgggtgatgg cgaccacaat atcaaagctc aaggtggacc tggactttga cagctcgtcc
2581 atcagtcaga ttcacctggt gctgttcgga tttcaggatg ccgtaaataa aattttgagg
2641 aaccacttaa ttaggcccca ctggatgttc gcgatggaca acatcatccg ccgagcggtg
2701 caggccgcgg tcaccattct catcccagag ctccgagccc actttgagcc tacctgtgag
2761 actgaagggg tagataagga catggatgaa gcggaagagg gctatccccc agccaccgga
2821 cctggccagg aggcccagcc ccaccagcag cacctgagcc tccagctggg tgagctcaga
2881 caggagacca acagactttt ggaacaccta gttgaaaaag agagagagta ccagaatctt
2941 ctgcggcaaa ctctagaaca gaaaactcaa gaattgtatc accttcagtt aaaattaaaa
3001 tcgaattgta ttacagagaa cccagcaggc ccctacgggc agagaacaga taaagagctt
3061 atagactggt tgcggctgca aggagctgat gcaaagacaa ttgaaaagat tgttgaagag
3121 ggttatacac tttcggatat tcttaatgag atcactaagg aagatctaag ataccttcga
3181 ctacggggtg gtctcctctg cagactctgg agtgcggtct cccagtacag aagggctcag
3241 gaggcctcag aaaccaaaga caaggcttga taccaatcag ctaagctgtg gcagagtgtc
3301 ccaccacgct acatgttttg ttaaagcttc tgttagtgta tacacgaatt ccgctgtgtt
3361 tacatattta aaaatgccat tgttcaatta atagtttaag aacttgtttt aaatactgtc
3421 ctgagtttct tttgaaacct gttatttata aacatagaac tgtgtgtatt gtgaaaacag
3481 tgagccttgg ttttgacctc ccggaatatt aggaaattca cttgtagtcc cagctatgca
3541 ggaggctgag gtgggaggat tgcttgagcc caggaggtgt ggaggctgca gtgagccatg
3601 atcacaccac tgcactccag cctgggcaac agagcccgac cctgtctcaa aaaaagtaca
3661 cccttcagca cttgctggaa tggtgaaaca aacaaggggt atttaacaaa catggaagct
3721 gggacactgc ctcagaactg gtatggtact tcaatttgag aaacacaaaa ctgatacgaa
3781 tgtgccttgt agttaatgtt tgatatgaac agaaaatagc ttcatattta tactgaatgt
3841 gtaagtagag aaaactaagt tatgtggcct ttgaaatgat tacaaaattg gaatgattac
3901 aaaagtctta ttttaaaatg gaactgtcct cttgcctgat aataaatatt gtatcttgta
3961 g
//