LOCUS AK131316 3857 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ16308 fis, clone PUAEN2006335.
ACCESSION AK131316
VERSION AK131316.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3857)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Tanigami,A., Fujiwara,T., Shibahara,T., Goto,Y., Hirao,M.,
Shimizu,F., Wakebe,H., Ono,T., Hishigaki,H., Watanabe,T.,
Ozaki,K., Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A.,
Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K.,
Nishikawa,T., Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Murakawa,K.,
Kanehori,K., Takahashi-Fujii,A., Oshima,A., Sugiyama,A.,
Kawakami,B., Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y.,
Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2004)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..3857
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2006335"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000249604"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 123..1829
/codon_start=1
/protein_id="BAD18479.1"
/translation="MGSEVVAGNSVGPTMGAASSGPLPPPPPPLPPSSDTPETVQNGP
VTPPMPPPPPLPGPAAETVPAPPLAPPLPSAPPLPGTSSPTVVFNSGLAAVKIKKPIK
TKFRMPVFNWVALKPNQINGTVFNEIDDERILEDLNVDEFEEIFKTKAQGPAIDLSSS
KQKIPQKGSNKVTLLEANRAKNLAITLRKAGKTADEICKAIHVFDLKTLPVDFVECLM
RFLPTENEVKVLRLYERERKPLENLSDEDRFMMQFSKIERLMQKMTIMAFIGNFAESI
QMLTPQLHAIIAASVSIKSSQKLKKILEIILALGNYMNSSKRGAVYGFKLQSLDLLLD
TKSTDRKQTLLHYISNVVKEKYHQVSLFYNELHYVEKAAAVSLENVLLDVKELQRGMD
LTKREYTMHDHNTLLKEFILNNEGKLKKLQDDAKIAQDAFDDVVKYFGENPKTTPPSV
FFPVFVRFVKAYKQAEEENELRKKQEQALMEKLLEQEALMEQQDPKSPSHKSKRQQQE
LIAELRRRQVKDNRHVYEGKDGAIEDIITALKKNNITKFPNVHSRVRISSSTPVVEDT
QS"
BASE COUNT 1191 a 769 c 773 g 1124 t
ORIGIN
1 aaagacagtc taccctggaa aaaaagattc atgagctaga gaaacaaggg accattaaaa
61 ttcagaagaa aggggatggg gatatcgcca tactgccagt tgtggcttct ggcacattgt
121 ccatggggtc agaagtggta gcaggtaact ctgtgggacc cacaatgggg gccgcttcct
181 caggaccctt gccccctcct ccaccaccac tgcctccctc atcagacaca cctgaaacag
241 tgcaaaatgg tccagtaaca ccacctatgc caccgcctcc tcctctccca ggccctgcag
301 ctgagactgt accagctcct cccttagcac ctccccttcc ctctgcacct ccgctgcctg
361 gaacatcttc acccacagtg gttttcaact caggattagc agctgtgaaa attaagaagc
421 caatcaagac gaagttcaga atgccagtgt ttaactgggt tgctctgaag cccaatcaga
481 tcaatggcac agtcttcaat gaaattgatg atgagcgaat tctggaggat ttaaatgtgg
541 atgaatttga ggaaatattc aagacaaaag cccaaggacc tgccattgat ctttcttcaa
601 gcaaacagaa gataccacag aagggatcaa acaaagtgac attactagaa gcaaacaggg
661 ccaaaaatct tgccataact ttaaggaaag ctggaaagac tgctgatgaa atatgtaaag
721 ctattcatgt atttgacttg aagacactgc ctgtggactt tgtggaatgc ttgatgcggt
781 tcctaccaac tgagaatgaa gtgaaagtgc ttcggctcta cgagcgggaa aggaagcctc
841 tggaaaactt gtcagatgaa gatcggttca tgatgcagtt tagtaaaatc gagaggctca
901 tgcagaagat gaccatcatg gccttcattg ggaactttgc tgaaagcatt cagatgctga
961 ctcctcaact acatgcgatt atagcagcat ctgtctctat aaagtcgtcc caaaaactca
1021 agaaaattct ggagatcatc ttagcccttg gaaactacat gaatagcagt aaaagaggag
1081 cagtttatgg atttaaactt cagagtttag atctgctctt agatacaaag tcaacagaca
1141 gaaagcaaac actgttgcac tatatatcca atgtggtgaa agaaaaatat caccaagtgt
1201 ccctgtttta taatgagctt cattatgtgg aaaaagctgc tgcagtctcc cttgagaatg
1261 ttttgctgga tgtcaaggag ctccagaggg gaatggactt gaccaagaga gagtacacca
1321 tgcatgacca taacacgctg ctgaaggagt tcatcctcaa caatgagggg aagctgaaga
1381 agctgcagga tgatgccaag atcgcacagg atgcctttga tgatgttgtg aagtattttg
1441 gagaaaaccc caagacaaca ccaccctctg tcttctttcc tgtctttgtc cggtttgtga
1501 aagcatataa gcaagcagaa gaggaaaatg agctgaggaa aaagcaggaa caagctctca
1561 tggaaaaact cctagagcaa gaagctctga tggagcagca ggatccaaag tctccttctc
1621 ataaatcaaa gaggcagcag caagagttaa ttgcagaatt aagaagacga caagttaaag
1681 ataacagaca tgtatatgag ggaaaagatg gtgccattga agatattatc acagccttaa
1741 agaagaataa tatcactaaa tttccaaatg ttcactcgag ggtaaggatt tcttctagca
1801 caccggtggt ggaggataca cagagctgat cttagaaacc aaccatacag acgagccgat
1861 gcggtgagga gaagcgtcag gcggcgcttt gatgatcaga acttgcgttc tgttaatggt
1921 gccgaaataa caatgtgaac ctgagactgg cctgcatgaa tacagggtgt gcgtgaatga
1981 aactgcccac atgaacttta tgtgctacga tttaactgca gccttgaaca cacacaaaaa
2041 tattcttaag ggctcagatt tagcaaacac ggaagaattt taaaatgagc tctcctttca
2101 acccttgtta acaagtgcct aaaaatggaa gtacctgttc agattaatca aagcaatagg
2161 atttgatttg attaggtatc tttttacacc agtatgttat ttttaaccaa aatgtaaagt
2221 tcttattaaa ctcattacct gccattgtga ttgtcccatc atggcccacc tggtttcctg
2281 atgttgtaaa taacatcaat gcatctgctg tgggtccttt gctgagatgt cttcgaagga
2341 attttgtttt agccatatcc atcaactttg tattttactt gcaatttgga agaaggaaag
2401 tcacatgatg aaactccttt tgtctataac caggccctgg caaagtgcaa acaggatgca
2461 actgcagtgg cacaaaggtc actcaatcct ttgtttccag tttcacattc tactacttct
2521 gcgctagaga acgatgctct gtgagaggca ttcactagta tgaatgtggg gatatagtgt
2581 ataagactta tttgcagtac tgtgttcttc agctagaggc agctttttaa ataatgcaag
2641 tgtatttatt agcattaaaa ttaacatctc agtaatcagc attagcattt ctgaggacca
2701 ttattaattc tgagaacaga aattggtgcc ttgcaaggaa gtttactagc tctatcaaca
2761 agcattcaag gttacatctg ctagcagagt agtgttagga acctggcctt actctcctct
2821 gacaatcgca attttttctt attttttata aattcaagaa gatacacttg gcatcgtgta
2881 tcgaggctaa gtttttcatg catttcccag actacttatg gagaattgca gtttaagttg
2941 ctgaaaagta ttaacatggt attaagctta aataatacgt aatgggacta gatggcccac
3001 taagccactg ttattttcct tcctctctgg cagggcactt gatccattcc aaagtcaaaa
3061 actggactga agctaaattt gtacttttca taatatacat tctgcttctg gcttatcttc
3121 ttggtacatc aatatattaa ttgtaaagtt tattgtatag tatttaaccg ctgaagttcc
3181 tattttatgt tgtgcttatg tgaacccctt ggtgaaggtc ccttttcctt ggatgtgtag
3241 ttatatgatc tttttaaatg tacagatatt ttgctataaa atcggtgcag ttttttatgg
3301 tttttacact tctctttaat tcccacctaa gcctctgggt aatattgtaa atattgtttt
3361 aaaatgcatc agcctatgct atacaatctg aatgttattt taacttatag ttttttttaa
3421 tatatatatt taactataag gacagtttag ggaacaagtt acctaccaca tttcacttta
3481 gtgtacctat ttacagaaag attaaactgc cacctgcggg cacattccca taaatgtgta
3541 ctttacttta aaaagaacat gccacgattt tgtctttctg tggactcaac attcacttcg
3601 attaaaaata gcaatttgac caagttggac ttccactaca aagcagctgt tttccaaagt
3661 tcaatgctga catatatgta tattaaaata attgcctatt tattaatcta caaatagaca
3721 acgttggcat gttcttttct gtttgtctat taatgggcct gcttcttagc aatattagaa
3781 tgttttataa aagcaattca tgttactttt ctggtctttt catggcatat gagcaaataa
3841 taaactattt acactac
//