LOCUS AK127719 3340 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ45819 fis, clone NT2RP8001363, highly
similar to Mus musculus signal peptide, CUB domain, EGF-like 1
(Scube1).
ACCESSION AK127719
VERSION AK127719.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3340)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Tanigami,A., Fujiwara,T., Shibahara,T., Goto,Y., Hirao,M.,
Shimizu,F., Wakebe,H., Ono,T., Hishigaki,H., Watanabe,T.,
Ozaki,K., Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A.,
Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K.,
Nishikawa,T., Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Murakawa,K.,
Kanehori,K., Takahashi-Fujii,A., Oshima,A., Sugiyama,A.,
Kawakami,B., Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y.,
Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..3340
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP8001363"
/clone_lib="NT2RP8"
/db_xref="H-InvDB:HIT000047592"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 711..2567
/codon_start=1
/protein_id="BAC87098.1"
/translation="METEAASTAVRTQTQAPRVVATRSTPSTQTVARASRRAQSITEA
ATGHARTQPLACDAAAPLDSHCSRTGRHAKTSTSAWSTTEAATTSAATPWAASSAAAG
RATSCSPTSAPARTSTSAPSSGPVTTSASTPQARAKETARQPLLDHCHVTFVTLKCDS
SKKRRRGRKSPSKEVSHITAEFEIETKMEEASDTCEADCLRKRAEQSLQAAIKTLRKS
IGRQQFYVQVSGTEYEVAQRPAKALEGQGACGAGQVLQDSKCVACGPGTHFGGELGQC
VPCMPGTYQDMEGQLSCTPCPSSDGLGLPGARNVSECGGQCSPGFFSADGFKPCQACP
VGTYQPEPGRTGCFPCGGGLLTKHEGTTSFQDCEAKVHCSPGHHYNTTTHRCIRCPVG
TYQPEFGQNHCITCPGNTSTDFDGSTNVTHCKNQHCGGELGDYTGYIESPNYPGDYPA
NAECVWHIAPPPKRRILIVVPEIFLPIEDECGDVLVMRKSASPTSITTYETCQTYERP
IAFTSRSRKLWIQFKSNEGNSGKGFQVPYVTYDEDYQQLIEDIVRDGRLYASENHQEI
LKDKKLIKALFDVLAHPQNYFKYTAQESKEMFPRSFIKLLRSKVSRFLRPYK"
BASE COUNT 741 a 1053 c 965 g 581 t
ORIGIN
1 gagcagcgag cagcgcctgc gggagcggcc ggtcggtcgg gtccccgcgc cccgcacgcc
61 cgcacgccca gcggggcccg cattgagcat gggcgcggcg gccgtgcgct ggcacttgtg
121 cgtgctgctg gccctgggca cacgcgggcg gctggccggg ggcagcgggc tcccagggtc
181 agtcgacgtg gatgagtgct cagagggcac agatgactgc cacatcgatg ccatctgtca
241 gaacacgccc aagtcctaca aatgcctctg caagccaggc tacaaggggg aaggcaagca
301 gtgtgaagac attgacgagt gtgagaatga ctactacaat gggggctgtg tccacgagtg
361 catcaacatc ccggggaact acaggtgtac ctgctttgat ggcttcatgc tggcacacga
421 tggacacaac tgcctggatg tggacgagtg tcaggacaat aatggtggct gccagcagat
481 ctgcgtcaat gccatgggca gctacgagtg tcagtgccac agtggcttct tccttagtga
541 caaccagcat acctgcatcc accgctccaa tgagggtatg aactgcatga acaaagacca
601 tggctgtgcc cacatctgcc gggagacgcc caaaggtggg gtggcctgcg actgcaggcc
661 cggctttgac cttgcccaaa accagaagga ctgcacacta acctgtaatt atggaaacgg
721 aggctgccag cacagctgtg aggacacaga cacaggcccc acgtgtggtt gccaccagaa
781 gtacgccctc cactcagacg gtcgcacgtg catcgagacg tgcgcagtca ataacggagg
841 ctgcgaccgg acatgcaagg acacagccac tggcgtgcga tgcagctgcc ccgttggatt
901 cacactgcag ccggacggga agacatgcaa agacatcaac gagtgcctgg tcaacaacgg
961 aggctgcgac cacttctgcc gcaacaccgt gggcagcttc gagtgcggct gccggaaggg
1021 ctacaagctg ctcaccgacg agcgcacctg ccaggacatc gacgagtgct ccttcgagcg
1081 gacctgtgac cacatctgca tcaactcccc aggcacgagc aaaggagacc gccaggcagc
1141 cgctgctgga ccactgccat gtgactttcg tgaccctcaa gtgtgactcc tccaagaaga
1201 ggcgccgtgg ccgcaagtcc ccatccaagg aggtgtccca catcacagca gagtttgaga
1261 tcgagacaaa gatggaagag gcctcagaca catgcgaagc ggactgcttg cggaagcgag
1321 cagaacagag cctgcaggcc gccatcaaga ccctgcgcaa gtccatcggc cggcagcagt
1381 tctatgtcca ggtctcaggc actgagtacg aggtagccca gaggccagcc aaggcgctgg
1441 aggggcaggg ggcatgtggc gcaggccagg tgctacagga cagcaaatgc gttgcctgtg
1501 ggcctggcac ccacttcggt ggtgagctcg gccagtgtgt gccatgtatg ccaggaacat
1561 accaggacat ggaaggccag ctcagttgca caccgtgccc cagcagcgac gggcttggtc
1621 tgcctggtgc ccgcaacgtg tcggaatgtg gaggccagtg ttctccaggc ttcttctcgg
1681 ccgatggctt caagccctgc caggcctgcc ccgtgggcac gtaccagcct gagcccgggc
1741 gcaccggctg cttcccctgt ggagggggtt tgctcaccaa acacgaaggc accacctcct
1801 tccaggactg cgaggctaaa gtgcactgct cccccggcca ccactacaac accaccaccc
1861 accgctgcat ccgctgcccc gtcggcacct accagcccga gtttggccag aaccactgca
1921 tcacctgtcc gggcaacacc agcacagact tcgatggctc caccaacgtc acacactgca
1981 aaaaccagca ctgcggcggc gagcttggtg actacaccgg ctacatcgag tcccccaact
2041 accctggcga ctacccagcc aacgctgaat gcgtctggca catcgcacct cccccaaagc
2101 gcaggatcct catcgtggtc cctgagatct tcctgcccat cgaggatgag tgcggcgatg
2161 ttctggtcat gaggaagagt gcctctccca cgtccatcac cacctatgag acctgccaga
2221 cctacgagag gcccatcgcc ttcacctccc gctcccgcaa gctctggatc cagttcaaat
2281 ccaatgaagg caacagcggc aaaggcttcc aagtgcccta tgtcacctac gatgaggact
2341 accagcaact catagaggac atcgtgcgcg atgggcgcct gtacgcctcg gagaaccacc
2401 aggaaatttt gaaagacaag aagctgatca aggccctctt cgacgtgctg gcgcatcccc
2461 agaactactt caagtacaca gcccaggaat ccaaggagat gttcccacgg tccttcatca
2521 aactgctgcg ctccaaagtg tctcggttcc tgcggcccta caaataaccg gggggagcgg
2581 ccctgcctgg gggtggcctg gtccgcggag ggtgcacctg ccctccacag tgggagctgc
2641 atgggcctcc acaccacctt gggaacccca tggcactgcc cttcagggaa gccgaccagc
2701 ccatggagac cgagcccagg cacccttcgg acccgctgcc cctgtgggag caccctgctt
2761 caggaagcct ccctccctcc ctctgcctcc cttccccagg acaccaagag cgccctctcc
2821 tgagccctgg cagaccgact gcaggtagca ggattgcagg accctctgcc tggcctggcg
2881 tttcaggaga gaggggaagt ggggcctgtg ctctgggagg cgtggtcatc cgagacagga
2941 gtccagggga gagaggaggg gacaaaggcg ccgtctgggg gaggtcgatg agcctgtgct
3001 ggcatccgcg ggccccacgc tttgccaact cctccagcca caggcaaggc cacggctccg
3061 ggctgttgcg ctctaagggt tctgtgattg gatggaacag agctgctggg gaggagactg
3121 gaagtttctg cattccttca acagaacatt taatgaagta ctctatatat atatataaat
3181 atatatataa atatatatat atacttctat ttgtgggtac tttaggaaaa tgccctttgg
3241 tcactgtaaa tatgaattgt gaccccatcc cttcccgcat gagcccagtg agtcccagca
3301 gctatcagcc tccctgaacg attaaacagc tcctcccagc
//