LOCUS AK127700 2618 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ45800 fis, clone NT2RI2028537.
ACCESSION AK127700
VERSION AK127700.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2618)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Oshima,A., Takahashi-Fujii,A., Tanase,T., Imose,N., Takeuchi,K.,
Arita,M., Musashino,K., Yuuki,H., Hara,H., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..2618
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2028537"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000047573"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 3..734
/codon_start=1
/protein_id="BAC87091.1"
/translation="MSGSPVQKPTLHRSISTKVLLAEGENTPFAEHCRHYEDSYRHLQ
AEMQSLKDQVQELHRDLTKHHSLIKAEIMGDVLHKSLQLDVQIASEHASLEGMRVVFQ
EIWEEAYQRVANEQEIYEAQLHDLLQLRQENAYLTTITKQITPYVRSIAKVKERLEPR
FQAPVDEQSESLQNTHDDSRNNAASARNNPGSVPEKREKTSEPKGNSWAPNGLSEEPL
LKNMDHHRSKQKNGGDVPTWREHPT"
BASE COUNT 671 a 686 c 647 g 614 t
ORIGIN
1 agatgtcggg gtcacccgtc caaaagccca cgctgcaccg gtccatcagc accaaggtgc
61 tgctggcgga gggcgagaac acgcccttcg cagagcactg ccgccactat gaggactcct
121 accggcacct gcaggcagag atgcagagcc taaaggacca ggtacaggag ctgcaccgag
181 acctcaccaa gcaccactcg ctcatcaagg cggagatcat gggagacgtc ctgcacaagt
241 ccctgcaact ggacgtgcag atcgcctcgg agcacgcctc cttagagggc atgagggtcg
301 tcttccagga gatttgggag gaagcctatc agcgagtggc taatgagcag gagatttatg
361 aagcccagct ccatgacctt ctccagctga ggcaggagaa tgcctacctg accaccatca
421 ccaagcagat cacgccctac gtccgctcca ttgccaaggt gaaggagcgg ctggagccca
481 ggtttcaggc acccgtggat gagcagtcag agagtctaca gaacacgcac gacgacagca
541 ggaacaacgc ggcctcagcc aggaataatc caggaagtgt cccggaaaag agagagaaga
601 catcagagcc taaaggaaac agctgggctc cgaacggcct ctcagaagag cctctactga
661 aaaatatgga tcatcacaga tccaaacaga aaaatggggg cgatgtcccc acatggaggg
721 aacacccgac ttagcaaatg ggaccggtcc ccagggtcag gctcttagag caggcacaag
781 actgggacac tggacagaag gttgttccca tgatggtttt ttttattttt tatttttgag
841 atggagtttc gctctgttgc ccaggctgga gtgtaatggt gcaatctcgg ctcactgcaa
901 cctctgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagtag ctgggattac
961 aggcgcctga caccacgccc cgctaatttt ttgtattttt agtagagatg gggtttcacc
1021 atgttggcca ggctggtctc aaacgccaga cctcaggtga tccacctgcc tcagcctccc
1081 aaagtgctga gattacaggg gtgagtcacc gcgcctggcc aatgttgttg ttgtttttaa
1141 gacagaattt cactctttgt tgcccaggct ggagtgcaat ggcgcaatct ctggctcacc
1201 gcaacctccg cctcccaggt tcaagcgatt ctcctacctc agcccccaga gtagctggga
1261 ttacaggcat gtaccatcac acccggctaa ttttttgtat tttaagtaga gagggggttt
1321 ctccatgttg gtcaggctgg cctcgaactc ccaacctcag gtgatccgcc cacctcggcc
1381 tcccaaaatg ctgggattac aggggtaagc cactgtgccc ggccggttat ttctttaaaa
1441 ggtaatcatt tgtcaagagt aaaacccaga agctctgaca ggccataatt tcagatcctt
1501 tggcttgggc agttttgatt ttccccgtgt ttgcatggca tgaagtcttc gtccttgtca
1561 cagtagcttg ggatgactcc cagtccacat ggaaaacatc agggagtgac aatccagcaa
1621 gaaatccctc gctagttcca cacctacgca ccgagcgtcg gtgtgccagg ccctgtgctg
1681 ggcagagtgt ggtatgtcag ggtgtgccgg ttttaggtaa caagactcca ccactgagtg
1741 gcacctgccc tattgcaaag gaatccagtt cctccggaat aacagtccca ctgttaacct
1801 ggtgctactg ggaagttcca cacagtaatc tgagcagtga ctcatggaag gatgaggaac
1861 gtttgctcca gcttctctcc ctttccagca agggcagagc tcctaaagcc aggggttagc
1921 acctggccag cttatgtggc agatggtctc agttacaact tcgctgcttt cccaaactcc
1981 tgcagccctc ctgagtccga cttccgttga tagcaaggca ctgggtggca gcaacctttt
2041 ttctagtagt tttttcccag cagttttcca tttctccaca gtatcctttt catttagagg
2101 agcttaataa atgcttttta aaaagtaacc cacgtgacgt aaaattttac aagtttttgt
2161 ggcaaaatga tgcccagata gtcacattta agcaaatatt cagcttgatt cagtgattaa
2221 cagcaaatgg gtctacgtgc taacatggca gcacattcaa cacataacac atcactcaca
2281 ttgacgtcca ctgtccctgc acctgctact tcaggggcac tgaggctcct gttccaaggc
2341 cttacaaacc tatgtggtgg cctgcagggc aaaaggaatt atcattacaa ctggttagag
2401 gtaggaattc agaaagaaat tgaggaggcc aaacacacgt cgtttgaggc taaaggctta
2461 agacgcttct tacccaagag tgacctcaga gtttcacatc ccagacaatc acactgtggt
2521 tgagtgaaat caagtgcagt tttatttaag aactggaaag aataatcagt atctgtgaaa
2581 gaaaatccaa tttagaatat ttaaataaac atttatgt
//