LOCUS AK127696 2315 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ45796 fis, clone NT2RI2012542.
ACCESSION AK127696
VERSION AK127696.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2315)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Tashiro,H., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..2315
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2012542"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000047569"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 652 a 543 c 524 g 596 t
ORIGIN
1 ctgtgacccg gaggcgctgg ggctgtcctg ggtcgtcatg cgagaaatcg tctcacccca
61 gccagtgtga cggaatgaga gcaaaagaaa gttttgatag cagcgtacga tttatattca
121 gctgtatgct gggcaggtcc aacttggcat ttcttggctg tatctctgaa ggaaagtcat
181 cttaatctct atgagcctcc atttcctcag ctgtaaaatg ggaagatctt cctcacactg
241 ctgtggccca gaaccatatc ctgctccaca agcctcgtcc ttggcaagaa gttgcacctg
301 cccagagaga aaaccttttg aaactggcac aaaggcactg gagagactgg cagggccccc
361 tacttgacga tctggctcat ccatctgcac actgaccacg ctaagagctg tctgcagtga
421 tggaaacctt ctctagctgt gctgtcctct atagtggcac cagctgcctg tggctactga
481 gcacttcaac tgtggctgca gagactatgt taaattgtat ttaatcataa ttaatttaat
541 tgtaaacaac tacatgtggc tagtggtttc tgtactgggc actgcaggtg cacatttcct
601 gctggccatg gacagtaagc tccataagag actaattgtg cctggtttgc ttataggctt
661 atcctcagca cctgcaccag cctagaatat gattggcact ctattaatta aaagttccca
721 ggtaaatgaa ttagtggatt ctctgtttcc tccactatgc cacaacctcc ttaagggcag
781 agatggtgtc ttcaaaaaaa aaaaattctg gtcaaaaaca cataagatct accaccttca
841 ccattgttgt gtccagttca gtagtattaa ctacacttac attgtgatac aacaaaagat
901 tgtgtctttt tacattctta ggacccagca cagtgtctgg cacataatct tagtaaacac
961 tggcagagca catgcctttg ccaaggccag aacagaggtt cagtgccatc cattaccacc
1021 ctttcacaca tccactgggg ctttaaaaac aacctggaat gaaagctgtt gagtctagag
1081 aaatgggcta caaagaaggt ttacaagata atctattaag aatagggaat acaggccggg
1141 cacggtggct cacacctgta atcccagcac tttgggaggc tgaggtgggt ggatcatttg
1201 aggtcaggag ttcgagacca gcctggccaa tgtggtgaaa tcctgtctct accaaaaatt
1261 atccaggcat ggtggtgcgt gcctgtagtc ccagctactt gggaggctga ggcatgagca
1321 tcgcttgaac ccgggaggca gaggttgcag tgagccgaga tcgcgccact acaccccagc
1381 ctgggtgaca gagcaagact ccgtctcaaa aaaataaaaa aacctaaaat aaagcttaat
1441 acacatgaca tatggattgg cagtcacaca taaatatgat tgataaggat acaaatattt
1501 ggaggtgctg cctgaccatc tgttgctgat tatgtggaga tcacttgtct aggaccctga
1561 gatagactga tgtttctaac tctgccacac acggtctctc ccaccctcca gacactttct
1621 tccttcagtt cagggaaccc tcaatacaca gtaagaatga agaggttgca ggtctgagct
1681 tgaggaggac tggactaaaa ggagtttagc cctgccaggg cattccgagg actgtgaaga
1741 tccggaatat tggccacagg catctatgta tttgaatcat atccattttt gagctcactc
1801 ctagcagagg agtcaaaact tacgttctgg cccccaggta catttccagt ctcttactca
1861 taaaattgtg acataccccc aggggtgccc aaaggggttt gtgaatcaag atagtttgag
1921 gagaatcgat tttctgatcc tcagtttttc tatgctcttt ttcctaaaag cgatattcct
1981 aaaaaggcat ccaagacaag gcatcccctt tcccatttgc tggggaagac agctttacaa
2041 gagaaagctg cccctctcac ccatgtgacc ccccactctg gggcactgcc ctaggggtag
2101 aaatctccaa ggcatcaaac aaagtgactc ctgggggtca agtgacagag gagcaagtct
2161 ttctgcaagt caagtggttt ccagctctgc tttcagcaaa attgaaagag gcactaatca
2221 aattgccagc ggatcataaa aaaacatttt gataataatg ttattaggca tataacttgg
2281 aagaagttca aagaattgaa tggcatcctc ataac
//