LOCUS AK127694 2583 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ45793 fis, clone NT2RI2009233.
ACCESSION AK127694
VERSION AK127694.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2583)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Oshima,A., Takahashi-Fujii,A., Tanase,T., Imose,N., Takeuchi,K.,
Arita,M., Musashino,K., Yuuki,H., Hara,H., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..2583
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2009233"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000047567"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 307..1119
/codon_start=1
/protein_id="BAG54551.1"
/translation="MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQ
PGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFL
LKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPR
EREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGR
DCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP"
BASE COUNT 548 a 713 c 699 g 623 t
ORIGIN
1 aaaatggagc gccgggcgta aggcaaagcc tggcaccgtc tgcgcggccg ctatctgctc
61 ccggagcgtg agtgcggggt gtggggcgtg cgcgtgcgcg ctcagagggg gctcaaggcg
121 agcgcgccgg gcagttgcgg gcgcgtggct gctgaggttg gcggcggtgc cgcgcgcccg
181 acgggccggt ggttgcgggg cctcccgcct cgacccgggc tgggggcagc cgtggcggcc
241 gccggggacc gcaaggggcg gaggaaagga gggggccggt cccggcacgc agaggagcag
301 ccgaccatgc cccgagacaa catggcctcc ttgatccaac ggatcgcccg ccaggcttgc
361 ctcaccttcc ggggcagctg gggcggccgc ggcgcttccg atcgcgacgc ggcttctggc
421 gcggaggcgc cgatgcagcc gggcttcccc gagaacctga gcaagctgaa gagcctcctg
481 acccagctcc gcgccgagga cttgaacatc gccccgcgca aggccacact gcagccgctg
541 ccgcccaacc tgccgccagt cacctacatg cacatctacg agacggacgg cttcagcctg
601 ggcgtgttcc tgctcaagag cggcacgtcc atcccgctgc acgaccaccc gggcatgcac
661 ggcatgctca aggtgctgta cggcaccgtg cgcatcagct gcatggacaa gctagacgcg
721 ggcggcgggc aacggccgcg ggccttgccg cccgagcagc agttcgagcc gccgctgcag
781 ccccgggagc gagaagccgt gcggccgggc gtgctgcgtt cgcgggccga gtacaccgag
841 gccagcggcc cctgcatcct cacaccgcac cgggacaacc tgcaccagat cgacgccgtg
901 gaagggcctg ccgccttcct ggacatcctg gccccgccct acgacccgga cgatggccgg
961 gactgccact attaccgggt gctggagccg gtcaggccca aggaggcctc cagctcggcc
1021 tgtgacctgc ctcgagaggt gtggctcctg gagaccccac aggccgatga cttctggtgc
1081 gagggagaac cctatccagg tcccaaggtc ttcccttgaa gccactggcg cccaggagcg
1141 gtgggccgaa gacgtgccct accctaccac aagggctgtg tctctacccc ctagcctggg
1201 cgttggatct actggaatga gcagcagccg cttcctcggc agccttggga agcacgggcg
1261 actggacagc agccgccggg cacggttatg ggggcggggt gggcggggag gctagattgt
1321 ttcctggtac tgtcactgcc actggggctt tgatttggag gaatggggca ggggactatc
1381 tgaagcgctt ccatcctaaa gccataatga aaatatcttc ctctcttccc cattctatac
1441 aaaatactaa gtggttttct tgctcccact ccctacccct tagttaaata gggtttattt
1501 tccactcatg cccttatgcc tttttttctt atagtttttt aacttattga ctgtgcatga
1561 cccagtggtt tgaattgttt ttagttcaag tcattggtaa aaactaggtt taaggagatg
1621 agctactgtt taaagtgagc tggcctgcct aattaattcc ttgtgaaaac taaatgattt
1681 tttcagtttg gggatcattc tcacaacata actatgcatg tagaggacaa gatttatttt
1741 ctttcctccc tttgcccagt agccacatct ggtttactca ggcagcatct actaagaaat
1801 tcagcacctg catatctctg tgacatggtc acttagagct tatcttccct atgaatctcc
1861 agatctgtga gtcgagcaga tttcatgttg cagattcacc tttaatgcaa agactgtatt
1921 atcctcacat gacttttttt cttgtcttac tgtaccttaa aaggtgatag agtaattctg
1981 tattttctaa cgggaagatt caaaggagct gaatgtgtta tgcttccaaa caactgaatg
2041 taaaacactc ctagccagtt gttgcattcc ctatatttat ttacttccaa tattttactg
2101 taaaagtagg gagaaatatt atgttgatag ttgtttcata ttctctcagg aactttaatg
2161 ttcccgactc gggtgattcc agctgtgttg ctggcagtgt tgtctcaacc ctctccctaa
2221 aatgactgag ccctgggttc atctaatgtg gttttcctta ggaagagata gaaggcacag
2281 aagatcacag ctagagaatt gagaattaac tatactacta gccattttag ggcaccaaaa
2341 cttgggatta aacacttcct acttcccact cccaactcct gaaatgaagt cttgctatct
2401 gtgactagtt ttatttttgt gcttttaata gtccgagcag tcttaccttg tttacacatg
2461 tattgacacc atttgcttca ggccatggag cactgtttct ccctttttac tatttatagg
2521 attccgtttt ttcacaagac ttttaataaa aagaaattgt agaaataaac acattaaaat
2581 ttg
//