LOCUS AK127627 2250 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ45725 fis, clone HCHON2009766.
ACCESSION AK127627
VERSION AK127627.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2250)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Oshima,A., Takahashi-Fujii,A., Tanase,T., Imose,N., Takeuchi,K.,
Arita,M., Musashino,K., Yuuki,H., Hara,H., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..2250
/cell_type="chondrocytes (HC)"
/clone="HCHON2009766"
/clone_lib="HCHON2"
/db_xref="H-InvDB:HIT000047500"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, chondrocytes"
/organism="Homo sapiens"
CDS 264..>2250
/codon_start=1
/protein_id="BAC87063.1"
/translation="MWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAK
LRCLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELNKSLLY
SAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSRT
IFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLI
AVLYRRRRKAIAEIWPKYCCFLAGIITFQYFICIGIPPAPCRDYPWRFKGASFNDNII
KWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDA
ASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACF
YFLLFGGDLLLKPIKSILRYWDWLIAYNIFVITMKNILSIGACGYIGTLVHNSCWLIQ
AFSLACTVKGYQMPAANSPCTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADI
KASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERML
SLTQEPGEGQDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETD
SEEEEEEELKKEDEEPPRRSAFQFVYQAWITDPKTALR"
BASE COUNT 600 a 499 c 552 g 599 t
ORIGIN
1 atctgactgc cagcctggag aagccggagg tgaggaagtt ggctgagcct ggggaggaga
61 agcttgaggg ctactctgaa aaagcccaga agggtgatct tgggaaagac agcgaggagt
121 cagaggagga cggagaggaa gaggaggaat ccgaggagga ggaagaaaca tcagacttaa
181 ggaacaaatg gcacctggtg attgaccgcc tcactgtgct cttcttaaaa ttcctggagt
241 attttcacaa gctgcaggtg ttcatgtggt ggattttgga gttgcacatc atcaaaatcg
301 tttcctctta cattatctgg gtttctgtga aagaggtgtc tctgttcaac tatgtatttt
361 tgatttcttg ggcttttgct ctgccgtacg ccaagctgcg ctgtctggct tcaagtgtct
421 gcacggtctg gacgtgtgtg atcatcgtct gcaaaatgtt gtaccagctt caaaccatta
481 agcctgagaa cttctctgtt aactgttcct tgccaaatga aaatcaaaca aacatcccct
541 ttaatgagtt gaacaagtct ctgctctaca gcgctcctat cgatcctaca gagtgggtcg
601 gcctgcggaa gtcttcgcct ctgctagtct acctgaggaa taacctcctg atgctggcta
661 tcctggcctt tgaagtcacc atttaccgcc atcaggaata ctatcgaggt cgaaataacc
721 tgacggcccc tgtgtctaga actatctttc atgacattac aagactacat ctagatgatg
781 gacttattaa ttgtgccaaa tatttcatta attacttctt ttacaagttt ggtctggaga
841 cctgtttcct aatgtcagtt aacgtcattg gccagcgaat ggatttctat gccatgatcc
901 acgcctgctg gctgatcgct gtcttatata gacgcagaag gaaagccatc gcagagatct
961 ggcccaagta ctgctgcttc ctggcaggca tcatcacctt ccagtatttc atctgcattg
1021 gcatcccacc tgctccttgc cgagattacc cgtggagatt caaaggtgcc agcttcaatg
1081 acaacatcat aaagtggctg tacttcccag atttcattgt gcggcccaac cctgtgtttc
1141 tcgtctatga cttcatgctg cttctgtgtg cctccttaca acggcagatt tttgaggatg
1201 agaacaaggc tgcagtgcga atcatggcag gtgacaatgt cgagatctgc atgaatcttg
1261 atgcggcctc cttcagccaa cataaccctg tgccagattt tattcactgc agatcttact
1321 tagacatgtc caaagtgatc atcttcagct acctcttctg gtttgtgctc accatcatct
1381 tcatcactgg gaccaccagg atcagcatct tttgcatggg gtacctggtg gcctgtttct
1441 acttcctgct ctttgggggc gatttgctgt tgaaacccat caagagcatc ctgcgctact
1501 gggactggct gatcgcatac aacatttttg tgattacgat gaaaaatatc ctgtcaatag
1561 gagcatgtgg atacattgga acattggtgc acaatagttg ttggttgatc caggctttca
1621 gcctggcctg cacagtcaaa ggctatcaaa tgcctgctgc taattcaccc tgtacactac
1681 ccagtgggga agcaggaatc atttgggaca gcatatgttt tgccttcctc ctgctgcaaa
1741 gaagagtttt catgagttat tattttctac atgttgtggc tgatataaaa gcttcccaga
1801 ttctggcatc aagaggagct gaacttttcc aggccacaat tgtaaaagct gtaaaggcaa
1861 gaattgagga agagaagaag tccatggacc agctgaagcg acagatggat cgcatcaagg
1921 ccaggcaaca gaaatataaa aagggtaagg agaggatgct gagcttgacc caggagccag
1981 gggaaggcca ggacatgcaa aaactctctg aagaggatga tgaaagagaa gcagacaaac
2041 agaaagccaa gggcaaaaaa aagcagtggt ggcggccttg ggttgatcat gcttccatgg
2101 tcaggagtgg agattattat ttgtttgaaa cggatagtga agaggaggaa gaggaagaat
2161 taaagaagga agatgaagaa cctccacgaa ggtcagcatt ccagtttgtt tatcaagcct
2221 ggattactga tcctaaaaca gcactccgac
//