LOCUS AK127432 4104 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ45524 fis, clone BRTHA2026290, highly similar
to Homo sapiens RasGEF domain family, member 1A (RASGEF1A), mRNA.
ACCESSION AK127432
VERSION AK127432.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4104)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nakagawa,S., Senoh,A., Mizuguchi,H., Inagaki,H.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Kanda,K., Wagatsuma,M., Murakawa,K., Kanehori,K.,
Takahashi-Fujii,A., Oshima,A., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..4104
/clone="BRTHA2026290"
/clone_lib="BRTHA2"
/db_xref="H-InvDB:HIT000047305"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="thalamus"
CDS 2508..3953
/codon_start=1
/note="highly similar to Homo sapiens RasGEF domain
family, member 1A (RASGEF1A), mRNA"
/protein_id="BAG54503.1"
/translation="MPQTSVAFSSILGPSCSGQVQPGMGERGGGAGGGSGDLIFQDGH
LISGSLEALMERLVPTVDYYPDRTYIFTFLLSSRVFMPPHDLLARVGQICVEQKQQLE
AGPEKAKLKSFSAKIVQLLKEWTEAFPYDFQDEKAMAELKAITHRVTQCDEENGTVKK
AIAQMTQSLLLSLAARSQLQELREKLRPPAVDKGPILKTKPPAAQKDILGVCCDPLVL
AQQLTHIELDRVSSIYPEDLMQIVSHMDSLDNHRCRGDLTKTYSLEAYDNWFNCLSML
VATEVCRVVKKKHRTRMLEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSK
VKTAKFDVLEHHMDPSSNFCNYRTALQGATQRSQMANSSREKIVIPVFNLFVKDIYFL
HKIHTNHLPNGHINFKKFWEISRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEA
LFVASFESEGPENHMEKDSWKTLRTTLLNRA"
BASE COUNT 834 a 1165 c 1242 g 863 t
ORIGIN
1 tcatgatggc tattgctgtg actggagcca agagcccagt gtgcaattct tccttgggaa
61 ggggctgcct ggtggtggac acagctgttc agatacacag gtttgcagct gcctgtcgaa
121 gtgcctgccc ctgctgttgg ggcaaggagg aagcaggtgc aagaacaagc gacctcaggg
181 ctgggtgggg gtggccggca ggggaccccg cagtggaagt gctgtcatca tcaccatttc
241 acagctggga aggtggaggg ctggccagat gcacccagga acaaggagtg ggcctgctgc
301 agggccggca ttcctgcccg ggaaaagcca tgtagggaac ctgcctctcc acttggcact
361 gagccctgga gaccctcctc gaagtgctcc gtggctgggc agggtgggcc ctgctaggtt
421 tgagctttcg ggggtagggg ctcatggggc atcccccaga cctgcagggt cttggtttgt
481 tggatgcagg agacctagcc ctgggccgtg tccccaggtg ggtctccagg taggcaggac
541 agaaggaacc cagagcaccc cacctctcag gtcagtgcgg ctccacacgg tccccctcag
601 gctctgctga ctccccaaag ggattctgag catgtgcaga acctctgctt gcctgggaca
661 cccctgcatg ctcctcctcc aggcccccac cctcgaaggc gtggccttcc cagcccctct
721 ctggctgctg accagtgggc tgggtcccca ggatggctcc tcaactccta ggcaggcctc
781 ctagcactga ggcaggtctc tctgcaggct ggacatggag tggccttgcc ctgtggggga
841 tcggctgggg ctgagtttgt ggggtgtcag agtgcatccc ccacatacta cctcctgacc
901 agagcagcct gcagtgggag aggccagtgg ctcggcaaat gccctctctg atgaccccaa
961 gagcttgggg tgaggaggcc caactgacca aggttaccac ctgctggtcg ccttttgaac
1021 ccagaactgt gtgatcttgg ggaggttgtc ccacagtgca ttggcctcag atgcctgaga
1081 cagtgctgag cagctgtggg caacaagggg tttgtgagcc cgccttcgtg acttcattgc
1141 acaaatggtc actgagtgcc accctgggcc agcgatagga tacacaggtg aatgatacag
1201 ccacttctgg ctttgtgggt caacagtcta ggttaaaccc agggaggctt cctggaggtg
1261 gtgacaccta ggctggactt ggaactcctg gggtttcctt agttgagttc cttcccactg
1321 ggcctcagtt tcaccatctg aaaaatgaag gattggctca catcagtggt tcctgggctg
1381 gagtggagag cgcgttgggg tacagaagca gtccacccca gagtttctga ccagcaggtc
1441 tggggctagg cctgagaatg tgcattctaa cttgtgtgag gcaccgctgc tggcctgagt
1501 ttgaagggag cattgtatga gtctgagcca tgacctccct gtcctggagt gggggcagca
1561 ctgagcctgg gggcctggac attccaccca gcgtcatcgt ggggcctttg gttgcgttct
1621 tgctctcctt gccaaagact ttctaagaat tccaaggaaa cctttaggtg tttggtggga
1681 aaaccagtga cgtctgggcc agatgctctt ttttccctgg tgggagggac agggacgtgc
1741 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgttgacgtg
1801 gggggtgtgg gcaggtggtc cgctctctcc ttgtaaggcc cctacagggc agaggcccac
1861 ggtcctgtca tggcactgaa gtaatcatgg caacaatttg ctctgtgcag tggggaaact
1921 aaggccaaaa tggcaggagg gtctcgcttc tccacctgac ctcagccgct ggcacctgtg
1981 ctgccttccc ttgagggcca ggaggttgag gctggctctg tgtgcaggca aggccacagg
2041 caggccacct ctggccctca ggctggcagt tgctgcatcg tccagtcccc acttggctcc
2101 tcccaggcat ggctggctgt ctggggcact ggggaaacag gcccagaggg cttcagactc
2161 ccctgggctt cacaagaact cccagctgca gggtgtggtg agaccctgag ccccatcaca
2221 gcgggccatg ctgtggagga cagtcccagt gtgggcaccg aggcctccag gaggcccagc
2281 tgctcagaca tctgcagctc aggggcactc agactcacag gccccaaagc cagttccaga
2341 tctctctcta gccctgccca cagccctgcc tcatactggt cagccacttg gcccaagacc
2401 ttcaccatgt ggagtggccc tgctcgccac tcctgctacc gcctgcctga gcaagctctc
2461 cttgggctgt ggcaagaccc accatccacg tggcagccac cgaaactatg ccccagacgt
2521 ccgttgcctt ctccagcatc cttgggccca gctgtagcgg acaggtgcag cctggcatgg
2581 gggagcgtgg aggcggggcc ggtggcggct ccggggacct catcttccaa gatggacacc
2641 tcatctctgg gtccctggag gccctgatgg agcgccttgt tcccacggtg gactattacc
2701 ccgataggac gtacatcttc acctttctcc tgagctcccg ggtctttatg ccccctcatg
2761 acctgctggc ccgcgtgggg cagatctgcg tggagcagaa gcagcagctg gaagccgggc
2821 ctgaaaaggc caagctgaag tctttctcag ccaagatcgt gcagctcctg aaggagtgga
2881 ccgaggcctt cccctatgac ttccaggatg agaaggccat ggccgagctg aaagccatca
2941 cacaccgtgt cacccagtgt gatgaggaga atggcacagt gaagaaggcc attgcccaga
3001 tgacacagag cctgttgctg tccttggctg cccggagcca gctccaggaa ctgcgagaga
3061 agctccggcc accggctgta gacaaggggc ccatcctcaa gaccaagcca ccagccgccc
3121 agaaggacat cctgggcgtg tgctgcgacc ccctggtgct ggcccagcag ctgactcaca
3181 ttgagctgga cagggtcagc agcatttacc ctgaggactt gatgcagatc gtcagccaca
3241 tggactcctt ggacaaccac aggtgccgag gggacctgac caagacctac agcctggagg
3301 cctatgacaa ctggttcaac tgcctgagca tgctggtggc cactgaggtg tgccgggtgg
3361 tgaagaagaa acaccggacc cgcatgttgg agttcttcat tgatgtggcc cgggagtgct
3421 tcaacatcgg gaacttcaac tccatgatgg ccatcatctc tggcatgaac ctcagtcctg
3481 tggcaaggct gaagaaaact tggtccaagg tcaagacagc caagtttgat gtcttggagc
3541 atcacatgga cccgtccagc aacttctgca actaccgtac agccctgcag ggggccacgc
3601 agaggtccca gatggccaac agcagccgtg aaaagatcgt catccctgtg ttcaacctct
3661 tcgttaagga catctacttc ctgcacaaaa tccataccaa ccacctgccc aacgggcaca
3721 ttaactttaa gaaattctgg gagatctcca gacagatcca tgagttcatg acatggacac
3781 aggtagagtg tcctttcgag aaggacaaga agattcagag ttacctgctc acggcgccca
3841 tctacagcga ggaagctctc ttcgtcgcct cctttgaaag tgagggtccc gagaaccaca
3901 tggaaaaaga cagctggaag accctcagga ccacccttct gaacagagcc tgaggcggat
3961 gcagcccgcg acgccagagg aagcacgtgc actaactggg tttaaatttt gactgatgtg
4021 ggttgagatg aggaggcctc actggttggg gtccattttg tatataactt ttatgagaaa
4081 aaaatggtaa ttatttcacg cgtc
//