LOCUS AK125339 3641 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ43349 fis, clone NT2RI3009158.
ACCESSION AK125339
VERSION AK125339.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3641)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Oshima,A., Takahashi-Fujii,A., Tanase,T., Imose,N., Takeuchi,K.,
Arita,M., Musashino,K., Yuuki,H., Hara,H., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..3641
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3009158"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000045212"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 228..1286
/codon_start=1
/protein_id="BAG54187.1"
/translation="MNTIVFNKLSGAVLFEDGGASERERGGRPYSGVLDSPHARPEVG
IPDGPPLKDNLGLRHRRTGARQNGGKVRHKRQALQDMARPLKQWLYKHRDNPYPTKTE
KILLALGSQMTLVQVSNWFANARRRLKNTVRQPDLSWALRIKLYNKYVQGNAERLSVS
SDDSCSEDGENPPRTHMNEGGYNTPVHHPVIKSENSVIKAGVRPESRASEDYVAPPKY
KSSLLNRYLNDSLRHVMATNTTMMGKTRQRNHSGSFSSNEFEEELVSPSSSETEGNFV
YRTDTLENGSNKGESAANRKGPSKDDTYWKEINAAMALTNLAQGKDKLQGTTSCIIQK
SSHIAEVKTVKVPLVQQF"
BASE COUNT 1148 a 710 c 743 g 1040 t
ORIGIN
1 aaaaataaag ccgagacgac ggcggtggcg gtggtgagcg cgctggagcc cgcgtggaga
61 acatgcggcg gggatgggag tgcgcctagt ctcgaggcgg gagagccagc cgccctgcag
121 ccggccgtcg gccccgcagc cacagaagcc gagccccgct gcggagctcc cggcggccca
181 gccccgaggc tctgcggccg cgccgcgcgt ccctaccaac cgacaccatg aacaccatcg
241 tcttcaacaa gctcagcggt gcggtgctgt ttgaggacgg aggcgcctcg gagcgggagc
301 ggggtggccg gccctacagc ggtgtcctgg acagtcctca cgcccgcccc gaggtgggca
361 ttcccgacgg cccgcccctc aaggacaacc tcggcctgag acaccggagg accggcgccc
421 ggcagaatgg cgggaaggtg aggcacaagc ggcaggccct gcaagacatg gcgcgacccc
481 tcaagcagtg gctttacaag caccgtgaca acccgtaccc caccaagacc gagaagatac
541 tcttggccct cggctcgcag atgacgctag tgcaggtgtc aaattggttt gctaatgcaa
601 gacgtcggct taagaatacc gttcgacagc cagatttaag ctgggctttg agaataaagt
661 tatacaacaa gtatgttcaa ggcaatgctg aacggcttag cgtaagcagt gatgactcat
721 gttctgaaga tggagaaaat cctccaagaa cccacatgaa cgaagggggc tataataccc
781 cagttcacca tcctgtgatt aaaagtgaga attcggtcat caaagcggga gtgaggccag
841 agtcacgggc cagtgaggac tacgtggcac cccccaaata caagagcagc ttgttgaacc
901 gttaccttaa tgactctttg agacatgtca tggccacgaa cactaccatg atgggaaaaa
961 caaggcaaag aaaccactcg ggatctttta gctccaatga atttgaggaa gaattagtgt
1021 ctccatcgtc atcagaaact gaaggcaact ttgtctatcg cacagacact ctggaaaacg
1081 gatccaataa gggtgaaagc gcagctaaca gaaaaggacc aagcaaggat gacacgtatt
1141 ggaaggagat caacgcagct atggccttaa caaatcttgc acagggaaag gacaaactgc
1201 agggaactac cagctgcatc atccagaagt cgtcccatat agcagaagta aagactgtca
1261 aagtgccgct ggtgcagcag ttttaagagc ttgttgcttt tcagatccaa tggatgttct
1321 ttccggtgtt ttcatcaacc ctcatcctaa gagccgaagc agggatgaaa atgactctct
1381 cccaaacctc ttcttatttt taattatccc aaatatatca tttagttgct tctataaaag
1441 acatataaat tataaaaaac tcattttaat caaaaatatt aacttatttt atgttactca
1501 aactatgcat aaaacatctg cattaccatt acagtaagtg ccttgcttcc cgacaataag
1561 ctccaacgtg ggcatagttg aacaagctat gcctcaaaat gccaacgcca tatgcttatt
1621 agcctgtgtg catcattcca gacgggccta atcattccag gactgaaacc agaatcgctg
1681 aaagcccttg aaatacattc aataattcat atgttaaaac ttggatatct gttcagccca
1741 aatgaaatct tccttttaaa aaacgtctac agtattgaaa attgttcaat gtgcttttca
1801 gagtgacggt gagaatttta tgcatgtatc ttgcctgcat atttgatatg ttacaaactt
1861 ccaaaattca aggtgcagcg atccacagaa cgttgtacat ttaagaagtg attccttcaa
1921 gctaatttaa aatttcattg aacacatggt gaccaggaaa actttttttc aagcactgtt
1981 ggaaagcacc acaaagccct ttagaattaa tctggatttg tttctcaagt tctgctgaag
2041 tttaaaaaaa aactttatta tacaaataac tcaaaatttt cctgtgtaaa actaaacctg
2101 tagttttaaa acataatcct gtttgcatta gagctcactg tctttttgtg atggaaactg
2161 tgttcgtatg gaatgactaa aaatctttta tttggtttgt ttcaaattac aattgctgat
2221 ggacaatttg tattgcagcg agaacaacag aatgaaagaa atgtatctct gtgcggctat
2281 acatatacat acataaaatt gatttttaaa tttaaaacat atggaaaaca aaacattgaa
2341 cagtttgaat tttgccaagt tggacattaa agtaaaaatg aagtgaaatc atgcattgaa
2401 agaaaacatt ttgtttctaa attagtctac cattgagtga gaataatcaa tatcaagaaa
2461 gaagactatc tttctcaact aaacaataat attccaatca gcttgggaag acctgaaact
2521 tgaataagca gtggaaatgc caaatataac agagggtatg tgctacagag aagtaaaaag
2581 ggtttgactt tttatgatgg gatttttttt tttctgggta tgtaatctat ttttttttta
2641 aactggaaag catttttgtc agtgtgaatg agggtcaata gtgcagccag tggtgacatt
2701 tttctttatt ttgcaaaatg cttttaaaac caaaggctgc tctagttgat ggacagtatc
2761 agtcttgatc taaattgtag gacacttttt catgtaacat aacatttggg gattgggttt
2821 atttagtgta atgaagataa tttgatataa aaatattttg tgtatatata tatattttta
2881 ctttgttttc taaattgctg tttgcagtaa cagtaagcgc aaagcaaaat atataagtta
2941 tgactgtatg atcagatgaa gtatgagttc ttttggtttg catccttaaa tagttagaga
3001 tctctgataa aaactttgga atctttgcaa aacaatacaa aaatgccaaa atgtgagcat
3061 gtcaatgaaa actaaagaca aatacttcac tctttttcat actattataa gttattctgg
3121 tattaaatat gttaataaaa gtgtttttgt tttgacatat ttcagttaaa tgaatgaatg
3181 ctggttgtat tttatttgaa tgagtcatga ttcatgtttg ccatcttttt aaaaaaatca
3241 gcaaatttct tctatgttat aaattataga tgacaaggca atataggaca actattcaca
3301 tgattttttt taataccaaa ggttggaaga ttttataatt aacatgtcaa gaagacttta
3361 tagtaagcac atccttggta atatctccaa ttgcaatgac tttttaattt attttttctt
3421 ttgctgcttt aacattttct ggatattaaa atccccccag tcctttaaaa gaatcttgaa
3481 caatgctgag ccggcagctg aaaatctaac tcataattta tgttgtagag aaatagaatt
3541 acctctattc tttgttttgc catatgtaat cattttaata aaattaataa ctgccaggag
3601 ttcttgacag atttaaaata aaagttaatt tctagacctc g
//