LOCUS AK125334 4153 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ43344 fis, clone NT2RI3008162, highly similar
to Rab effector MyRIP.
ACCESSION AK125334
VERSION AK125334.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4153)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Oshima,A., Takahashi-Fujii,A., Tanase,T., Imose,N., Takeuchi,K.,
Arita,M., Musashino,K., Yuuki,H., Hara,H., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..4153
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3008162"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000045207"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 81..2354
/codon_start=1
/note="highly similar to Rab effector MyRIP"
/protein_id="BAG54185.1"
/translation="MQKSLRPIGLLRAQSLEWFYNNVKSRFERFGSAKVLKNLYRKHR
LESGACFDILGGSLFESNLENEGSISGSDSTFYRQSEGHSVMDTLAVALRVAEEAIEE
AISKAEAYGDSLDKQNEASYLRDHKEELTEELATTILQKIIRKQKSKSEQQVEEEPGW
PHPQSCSTKVADEGTSASPGGYRAPAALWRSQSAFSITGEEALKTPPVEAPSRQPRDQ
GQHPRAESALPSWKSVDRLDETNLAPVLQSPDGNWVALKDGAPPPTRLLAKPKSGTFQ
ALEVASSVASAYDEMGSDSEEDFDWSEALSKLCPRSRALPRNPQPQPTQAQSSDQGPI
AASPSSALSPNPEAMCSDSETSSAGSSREVGHQARLSWLQRKAPRNPAAEKMRLHGEL
DVNFNPQLASRETSDSSEPEEAPHTTDRRARRWRRARLGSEEPSKEPSSPSAQLRDLD
THQVSDDLSETDISNEARDPQTLTDTTEEKRRNRLYELAMKMSEKETSSGEDQESEPK
TESENQKESLSSEDNSQSVQEELKKKFSAVSLCNISTEVLKVINATEELIAGSTGPWE
SPQVPPDRQKGMFPRGTDQVRLDEQLTSLEENVYLAAGTVYGLETQLTELEDAARCIH
SGTDETHLADLEDQVATAAAQVHHAELQISDIESRISALTIAGLNIAPCVRFTRRRDQ
KQRTQVQTIDTSRQQRRKLPAPPVKAEKIETSSVTTIKTFNHNFILQGSSTNRTKERK
GTTKDLMEPALESAVMY"
BASE COUNT 1186 a 1012 c 1008 g 947 t
ORIGIN
1 attttgatgt cctcaactgc agtaggagcc atctccctga cttgttctga cctgacttgt
61 tccctaccgt aatctcctgg atgcagaagt ccctcaggcc catcgggctt ctgagggccc
121 aatctctgga atggttctac aataatgtga agagccgctt cgagcgcttt ggcagtgcca
181 aggttctgaa gaacctgtac aggaagcacc ggctggagag tggcgcgtgc ttcgacattc
241 taggaggaag cctttttgag tcaaacctgg agaatgaagg aagcatttct ggcagtgatt
301 caacatttta taggcagtca gaaggacata gtgtgatgga caccttggct gtggccctac
361 gggtggctga agaggccatt gaggaagcaa tttccaaagc agaggcatat ggggacagcc
421 tggacaagca aaatgaggcc agttacctgc gggaccacaa ggaggagcta actgaggaac
481 tggccacgac aatcctgcag aagattatac gaaaacagaa gagcaaaagt gagcagcaag
541 tggaagaaga gccaggatgg ccacatcccc agagttgcag cacaaaggtg gcagatgagg
601 ggacctcagc atcccctgga ggctaccgtg ctcccgctgc cctctggagg tcccagtctg
661 ccttctcaat cactggagaa gaagccctga agacccctcc agtggaggct ccatcgaggc
721 agccaaggga ccaaggccaa cacccgagag cagagtctgc tctgcccagc tggaagagtg
781 tggacaggct ggatgaaaca aacctggccc cagttttgca gagccccgac gggaactggg
841 tggccctgaa ggatggcgct ccacccccca cccgactact ggccaaacct aagagcggga
901 cgtttcaggc cctggaggtg gcctccagtg tggcatctgc ctacgatgag atgggctccg
961 atagcgagga agactttgac tggagtgagg ccttgagcaa gctgtgtccc aggtcccggg
1021 ccctgcccag gaacccccag cctcagccca cacaggccca gagctctgac caaggcccca
1081 tagctgcctc cccatcctct gcactctccc ccaaccctga ggccatgtgc tctgactcgg
1141 agacctcctc cgcaggctct tcccgagaag ttgggcacca ggccagactg tcctggttgc
1201 agaggaaggc ccccaggaac cctgcagctg agaagatgcg cttgcatggg gagctggacg
1261 tgaacttcaa cccccagttg gccagcaggg agacctcgga cagcagcgag ccggaggagg
1321 ccccccacac cacagaccgg cgggccagga ggtggagaag agcccgattg ggctcagaag
1381 agccaagcaa agaaccatct tcccccagcg cccagctccg ggatctagac acacatcagg
1441 tgtcggatga tttatcagag acagacatca gcaatgaggc tcgggacccc cagactctca
1501 cagacaccac agaggagaaa cggagaaaca ggctgtacga gttagcaatg aaaatgagtg
1561 aaaaggagac ttcttcaggg gaggatcagg agtctgagcc caagacagaa tctgagaacc
1621 agaaggaaag tctgtcctct gaagacaaca gccagagtgt ccaggaagag ctgaagaaga
1681 agttttctgc tgtttctctc tgcaacatct ccacagaagt cctgaaagtc atcaatgcca
1741 cagaggagtt gatagcagga tctacagggc cctgggagtc cccacaagtc cctcctgaca
1801 gacagaaggg gatgtttcct cgtgggacag accaagtgag actggatgag cagctgactt
1861 ccctggaaga aaatgtatac ctggcagcag gcactgtgta tggactggag acccagctga
1921 ctgagctaga agatgccgcc cgctgcatcc acagtggcac tgatgagacc catctggcgg
1981 atctggagga ccaggtggcc acggctgcag cccaagtcca ccatgctgaa ctccagattt
2041 cagatattga gagccggatt tcagccctga ccattgcagg attaaacata gcaccatgtg
2101 tgcgcttcac aagaagacgg gatcagaagc aaaggaccca ggtacaaacc atagatacat
2161 caaggcagca aaggaggaaa ctgcctgctc caccggtgaa agctgaaaaa attgagacat
2221 cttcagtgac taccattaaa acatttaacc acaacttcat tctccaaggc tcctcaacaa
2281 acaggactaa ggaaaggaaa ggcaccacca aggatttgat ggagcctgct ctggagtcag
2341 ctgtgatgta ctgacaccat ggaattccac tgccagtgac ccactgcctc cggccgtaca
2401 cgacagtgcc ttgacccaac agccatcgag tactgtatgt atttccacct gaggagaagg
2461 cctggggagg ccacagtgca ccattgcaca gggctgtcct gatacctcat ccagaaagcc
2521 gtctcagact tcagcactgc ggtcttgccc actctctgcc ttaggctccc aggggaatcc
2581 aagacagaaa atgaagacac tggcttccaa cagcagcgct ccatgtttaa gatacatatt
2641 ttccctgttt gctttgctac tgtatgttga ctttaagatc tttttttaaa tacatttgat
2701 tcagctagta ttccatgtca acaatttgtc caaaggaaaa ctgctggagg gaggtggagg
2761 gaggaaggtg ggaattatta tttaatacat cattaatgct tattaatctc tcacaagcat
2821 ctttgtcttg caaatcctaa gggaaaagca agtccctgca gtgagcacta gggacagtct
2881 aatttgggga ttgctcaacc atcaagactg caggtctccc ttcagccacc tccttcctgc
2941 taaaagctta gcctaccaca ctaccagtca ttcccatcgc tctgcaatca caagccacag
3001 gatgagaagt tctgactcac tcatgccatg cccagggcta tctgaaacaa tgtctcatta
3061 agaatttagg gttcttccat gggcttactg acagttgccc agatctgaag gggaaagggt
3121 cttgagaaag accatcactg gctcaacttt agggcactgt ccagagtcaa catgatgtgg
3181 tttagcagtg atcacatcta aacaaagttt aggtaaatga attatcgcag agaaaaacca
3241 catgagaaaa tttttgtact ccaaatttac ttcccaataa atattcagca aagtagtaaa
3301 atgaccttaa agataaaaat gattagggaa tagccttaga aaatttatag gtataaaaaa
3361 ttcaaggaca aactgtgcat ttaatggaca caagaattga ctctaactcc atgtctgtgg
3421 tttctttgaa cccatatcaa atgtatgact atttagagtg tttataagag ataatggaac
3481 tgaactttca ctcaattaat tgggcattaa caaccttctt ttatgtttgt tcctgatata
3541 gtctgaatct taggaagaag gtaaaagaaa ggaggcaaga gaatagttat gatgaatatg
3601 tgttaagtgc ctgctctgaa ggaggcaatg tccttctcat ttgaatcctt atggcaacct
3661 tattcaatag gttttcccat atttcagatt taataactga aggccagaga gattaatttg
3721 ccaaagccac acctttatgc taattatgat tggaatgcat cacaaaagcc taactctgtt
3781 gttttcaacc tctacgttat tttgctgcta tgtgcatttc cagatctgat tttctgctaa
3841 cttgtgtgct atgatccact cctgatgggg gtctacatta atcttccagt actccttgct
3901 gatgctgtgt tatgtgtcat ctaacagaaa tgactccttt gaaataagta aatctttggc
3961 tttttgttcc gttggtgtga ttcaaagcaa aacaaacaaa caaaaacaaa ttttaagaac
4021 acaacaaaaa agatttgact tccgaataga atgttttctt taagaggcat gaaaagcaac
4081 tattgttgtg ttacagtgtt aaaaatattc agttttcttt gacaaaaatg tgtactgtgt
4141 aagccttgca aac
//