LOCUS AK125323 4291 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ43333 fis, clone NT2RI3006376.
ACCESSION AK125323
VERSION AK125323.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4291)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Oshima,A., Takahashi-Fujii,A., Tanase,T., Imose,N., Takeuchi,K.,
Arita,M., Musashino,K., Yuuki,H., Hara,H., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Sugiyama,A., Kawakami,B.,
Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K. and
Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..4291
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3006376"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000045196"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 152..4081
/codon_start=1
/protein_id="BAC86129.1"
/translation="MGTHHHAQLIFVFLVEMRFHRVDQAGLELLTSGNSPASASRSAE
ITVVSQHAQPGFLYQWLEADRHGKSQGAANTTSGENFDQSPLKRTFKSKVLAHYPQNI
EWNPFDQDAVNMLCMPKGLSFRTQTDNKDPQFHSFIITREDGSRTYGFVLTFYEEVTS
KQICTAMQTLYQMHNAEHYSSVYASSSCSMDSLASSLDEGDTTSLLKLQRYNSYDISR
DTLYVSKSICLITPLPFMQACKKFLIQLYKAVTSQQPPPLPLESYIHNILYEVPLPPP
GRSLKFYGVYEPVICQRPGPSELPLSDYPLREAFELLGLENLVQVFTCVLLEMQILLY
SQDYQRLMTVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRS
KLELPQEANLCFVDIDNHFIELPEEFPQFPNKVDFIQELSEVLVQFGIPPEGSLHCSE
STSKLKNMVLKDLVNDKKNGNVCTNNISMYELLKGNETIARLQALAKRTGVAVEKMDL
SASLGEKDKDLKLHCEEAELRDYQLNVQLREVFANRFTQMFADYEAFVIQTAQDMESW
LTNREQMQNFDKASFLSDQPEPYLPFLSRFIETQMFATFIDNKIMSQWEEKDPLLRVF
DTRIDKIRLYNVRAPTLRTSIYQKCSTLKEAAQSIEQRLMKMDHTAIHPHLLDMKIGQ
GKYEQGFFPKLQSDVLATGPTSNNRWVSRSATAQRRKERLRQHSEHVGLDNDLREKYM
QEARSLGKNLRQPKLSDLSPAVIAQTNCKFVEGLLKECRMKTKRMLVEKMGHEAVELG
HGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLIQFQDREEKQEHLAE
SPVALGPERRKSDSGVMLPTLRVSLIQDMRHIQNMSEIKTDVGRARAWIRLSLEKKLL
SQHLKQLLSNQPLTKKLYKRYAFLRCEEEREQFLYHLLSLNAVDYFCFTSVFTTIMIP
YRSVIIPIKKLSNAIITSNPWICVSGELGDTGVMQIPKNLLEMTFECQNLGKLTTVQI
GHDNSGLLAKWLVDCVMVRNEITGHTYRFPCGRWLGKGIDDGSLERILIGELMTSASD
EDLVKQCRTPPQQKSPTTARRLSITSLTGKNNKPNAGQIQEGIGEAVNNIVKHFHKPE
KERGSLTVLLCGENGLVAALEQVFHHGFKSARIFHKNVFIWDFIEKVVAYFETTDQIL
DNEDDVLIQKSSCKTFCHYVNAINTAPRNIGKDGKFQILVCLGTRDRLLPQWIPLLAE
CPAITRMYEESALLRDRMTVNSLIRILQTIQDFTIVLEGSLIKGVDV"
BASE COUNT 1231 a 995 c 966 g 1099 t
ORIGIN
1 acggaccccg cctggcgcgc cggccccttc gcctgcagcc gcactcggag gcggccggct
61 gaagtgcagt ggcatgatct cagatcacta caacctccac ctcttgggtt caagtaattc
121 tcctgcctgg ccttcctgag taactgggat tatgggcacc caccaccatg cccagctaat
181 ttttgtattt ttagtagaga tgaggtttca ccgtgttgat caggctggtc tcgaactcct
241 gacctcaggc aattcacctg cctcggcctc ccgaagtgct gagattacag ttgtgagcca
301 acatgcccag ccaggatttt tgtaccaatg gctagaagca gatcgtcatg gcaagagcca
361 aggtgctgca aatacgactt caggcgaaaa ttttgaccag agtcctttga aaagaacatt
421 caaatccaaa gttctcgccc actatcctca gaatatagaa tggaaccctt ttgatcaaga
481 tgcggtgaac atgttgtgca tgcctaaagg gctatctttc aggacacaaa cggacaataa
541 agacccccag tttcattcat ttataattac cagggaagat ggttctcgca cctatggttt
601 tgttctcact ttttatgaag aagttacaag taagcaaatc tgcacagcaa tgcagacact
661 ttaccagatg cacaacgctg agcattacag cagtgtgtat gcttcatctt cctgcagtat
721 ggactcattg gcaagtagtc ttgatgaagg agatacaact tcccttttga aactccagcg
781 atacaactcc tatgatatta gcagagacac cctgtatgtt tcaaaaagta tatgcttgat
841 cacaccgtta ccattcatgc aggcctgcaa gaaattcctt atccagcttt acaaggctgt
901 tacctcacag cagccaccac ccttgccact tgaaagctat atccacaata ttctttatga
961 agtacccctt ccacctccag ggaggtcact gaaattttat ggtgtttatg aacctgtcat
1021 ctgccagagg cctgggccca gtgaactccc cctctctgat tacccccttc gggaggcatt
1081 tgagctcctg ggattagaga acctggtgca ggtgtttacc tgtgttcttt tagagatgca
1141 aatccttctc tactcacaag attatcaacg cctgatgact gtggcagaag gcatcaccac
1201 acttttgttc ccatttcaat ggcaacatgt ttatgtgccc attctacctg cttctctgct
1261 acattttctt gatgctcctg tcccttatct gatgggcctt cagtcaaaag aaggaactga
1321 ccgttctaaa ctagaacttc ctcaagaggc taatttgtgt tttgtggaca ttgacaacca
1381 ttttattgag ttgcctgaag aatttccaca gttccccaat aaagtggatt ttatccaaga
1441 actctctgag gttcttgttc aatttgggat ccctcctgag ggcagcctac attgcagtga
1501 gagtaccagc aaactgaaga atatggttct gaaagacttg gtcaatgaca aaaagaacgg
1561 caatgtctgt actaataaca tcagcatgta tgagttactg aagggcaatg aaaccatagc
1621 ccgcttgcag gctctggcca agcgtactgg tgtggctgtg gaaaaaatgg acctctctgc
1681 ttctctgggt gaaaaagaca aggatttaaa actgcattgt gaagaggcag aactaaggga
1741 ctaccagctc aatgtacagc tccgagaggt ctttgctaac cgttttacac agatgtttgc
1801 agattacgaa gcatttgtca ttcagactgc ccaggacatg gaatcctggc tgaccaaccg
1861 ggaacagatg cagaactttg acaaagcttc ctttctgtct gaccagcctg agccttacct
1921 gccatttctt tcacgcttca ttgaaacaca gatgtttgcc acctttattg ataataaaat
1981 tatgtctcag tgggaagaga aagatccttt gcttcgggtc tttgacactc ggattgataa
2041 gataaggctg tataatgtaa gggcacccac cttgcggaca tctatatatc agaaatgcag
2101 cactttaaaa gaagcagccc aatcaattga gcagagactg atgaaaatgg atcacactgc
2161 aatccaccca catctacttg atatgaaaat tggtcaaggc aaatatgagc aggggttctt
2221 tccaaagtta cagtccgatg tcttggcaac aggaccaacc agtaacaatc gctgggtaag
2281 tcggagtgcc actgcacagc gcaggaaaga acgccttcgc cagcattctg agcatgttgg
2341 gctggacaac gacttgaggg agaaatatat gcaagaggca cgaagtttag gaaaaaacct
2401 gaggcaaccc aaactgtcag acctctctcc tgcagttatt gcacagacca actgtaaatt
2461 cgtagaaggc ttattaaaag aatgtagaat gaagacaaag cgcatgttgg tggagaagat
2521 gggacatgaa gcggtggaac ttggccatgg agaagcaaac atcaccggcc tggaggagaa
2581 caccttgatc gccagccttt gtgacctgct ggagaggata tggagccatg gcttgcaggt
2641 caagcagggg aagtcggctt tgtggtcaca tttaattcaa tttcaggaca gagaagagaa
2701 acaagagcac cttgcagaat caccagttgc cctcggacca gaaagaagaa aatctgactc
2761 aggagttatg ttgccaacgc tcagggtctc tcttattcag gacatgaggc atattcaaaa
2821 catgagtgag atcaagactg atgttggacg agctcgggcg tggataagac tgtctctaga
2881 aaagaagctc ttgtcccagc atcttaagca gttgctttct aaccaaccac tcaccaagaa
2941 gctttataag cgatatgctt ttctacgttg cgaagaagaa agagagcagt ttctttacca
3001 ccttctttct ctcaatgctg tggactattt ctgcttcacc agtgtgttca ccactatcat
3061 gattccgtat aggtcagtga tcatcccaat caaaaagctg agcaatgcaa taatcacatc
3121 aaacccttgg atctgtgtat caggagagct gggagacaca ggagtaatgc agattcccaa
3181 aaacctcctc gaaatgacct ttgagtgcca gaacttgggg aagctgacca ctgttcagat
3241 tggtcacgat aactcaggac tgttagccaa atggctagtg gattgtgtca tggtcagaaa
3301 tgaaatcaca ggacatacat acagattccc atgtgggcgg tggctgggga aaggcattga
3361 tgatgggagc ctggagagaa ttcttattgg agagttgatg acatcagcat cagatgaaga
3421 tctagtaaag cagtgtcgga ctccacccca gcagaagtca cccaccacgg ctaggagatt
3481 gagcatcact tcactgacag gaaaaaacaa caaacccaat gctgggcaga tacaagaagg
3541 aattggagaa gctgtgaaca atattgtgaa acattttcat aaacctgaaa aagagagagg
3601 aagcctcacc gtgttgctgt gtggagaaaa tggcctggtt gcagcccttg agcaagtttt
3661 ccaccatggg ttcaaatctg cccgcatctt tcacaagaat gtcttcatct gggacttcat
3721 agagaaagtg gttgcttatt ttgaaacaac tgaccagatt ctagataatg aagatgatgt
3781 ccttattcag aaatcatcct gcaaaacctt ctgccactac gtaaatgcta ttaatactgc
3841 acccaggaac attgggaagg atggcaaatt ccagatttta gtttgccttg gaacaaggga
3901 tcgcctgctc ccacagtgga ttccattgtt agctgagtgt cctgccatca ctcgaatgta
3961 tgaagagagc gctctcctgc gagaccgcat gactgtcaac tcccttatcc gaattctgca
4021 gaccattcag gacttcacca tagtcctaga aggatcactc atcaaaggag tggatgtgta
4081 acccaactgg ctagaaactc tcagtccaaa ccttgctcct tccccaacta ggggaccgat
4141 ttggacttgt ctgacagtag tgagtcactg caggggcagc caaacatatg ccccatttgg
4201 aacaatcctc actctacaga caaggcaaaa tgttgtattg tagttcattt gaacctggaa
4261 tttagtataa aatagagtat tttcatgtgt t
//