LOCUS AK125321 3862 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ43331 fis, clone NT2RI3006132.
ACCESSION AK125321
VERSION AK125321.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3862)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Suzuki,O., Sasaki,N., Aotsuka,S., Shoji,T., Ichihara,T.,
Shiohata,N., Matsumoto,K., Hirano,M., Sano,S., Nomura,R.,
Yoshikawa,Y., Matsumura,Y., Moriya,S., Chiba,E., Momiyama,H.,
Onogawa,S., Kaeriyama,S., Satoh,N., Matsunawa,H., Takahashi,E.,
Kataoka,R., Kuga,N., Kuroda,A., Satoh,I., Kamata,K., Takami,S.,
Terashima,Y., Watanabe,M., Sugiyama,T., Irie,R., Otsuki,T.,
Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..3862
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3006132"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000045194"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 82..684
/codon_start=1
/protein_id="BAC86127.1"
/translation="MAGWGSYSVTATPFTAVIMSMLSTSLESLVLSSVLIRFMALNPR
SQQEVEMGTQTTTAMSLLSRGKRQCWARAFLQSMENGRCWAMVFRPSHSSSRASSLYP
VGREHSKYLARLRMSSFPKLASSIPLGQAMVSNTSMFFSCGGRSASNLCWRYSIPVRT
VLSLSAWNRCSATSSLSKQMPWSRPSLKLALNSSSLGPAW"
BASE COUNT 839 a 1134 c 1030 g 859 t
ORIGIN
1 gcaggggagc gggctggcac ctgggcacag gtgtagacat ggctgaaatg ccggcctgag
61 gaggtcaggt accagtgctg gatggcgggc tgggggtcgt actccgtgac ggccacgccg
121 ttcacagctg tcatcatgag catgttgagc acctcgttgg agagcttggt cctctcatcg
181 gtcctgattc ggttcatggc cttgaacccc cgctcacagc aagaggtgga gatgggcaca
241 cagaccacca cggccatgag cttgcttagc agggggaagc ggcagtgctg ggccagggcg
301 tttttgcaga gcatggagaa cgggaggtgc tgggcaatgg ttttcaggcc cagccactcc
361 tccagcagag cttcctcact gtatcctgtt gggagggagc actcgaaata cctggccagg
421 ttgagaatgt catcattccc aaaactggca agttcaatcc cacttggcca ggccatggtg
481 tcaaacacct ccatgttctt cagctgtggg ggtcggtctg cgtcaaacct ctgctggagg
541 tactcaatcc ccgtcaggac tgtcctctcc ctatccgcct ggaaccgctg ttccgctacc
601 tccagtttgt ccaagcagat gccgtggagc cgcccatcct tgaagctggc gttgaattct
661 tcctctttgg gccctgcctg gtgacggagg ctctccagtg ccacgtaggc gcggcccagc
721 gtggcgttca cctctgtaat cagcacgatc tccttctggc acacctcgga cagaggcctg
781 tagatgctca ggaagtccaa caggaagtgg cagaacttga caaagtggaa gccgcgcatg
841 agcttcagca tccctttggc ccggtgccca atctggcccc cagcctctgc caccctctgg
901 aggtgcctgg ccagggcggg ccagctcacg agcagcgcgt gcagcgtgcg cctcctgctg
961 gccacccagc ggaccgcatt cagatccttc aggcggatga tctcctgctc cagaggcgcc
1021 gcaccttcct gcagctcgtt cagcctcttg tttgaggact gataaaactt gaagacggtg
1081 cggatgtgcc ggtcacactt cttcaccaga tcgatgctcc cgcaggcgtc caccacagcc
1141 aggtgcagcc ggtgggccac gcagtggaca ggcagcagct gcgggatgac ctcctggaac
1201 ttttccacaa ggcctcctct gcagctcaac atggctgagc catccgtccc cagccccacc
1261 acccagccag gcttccggaa ggggatgtcc agctcatcca gggcagaaac gatggtctcg
1321 aagtacccat ctgctgtctc actgtagaga ggggccagag tgatgtagga ctctttcacc
1381 tccatctgct tgaagtagcg gatgtaaatc cccacgcagg cctgctcgga ggcgtcggtg
1441 gagctgtcca gcagcacgct cacacagggc gagttccgca cgtcctccag gatctccctc
1501 ttcagggtct ctgagatgta cttgatgaac tgagtgcacg ccgtgcgatt gcggtacttg
1561 cctaatatca cggtccccgt gctttggagg agctgcagga tcttctcaaa gtcattcagg
1621 ggccttgagt ggtatgcaat ggagtaggcg gcattgaaaa agtgctccat gttggccatg
1681 aggtcgctgg agatctctgg aacgagggca gtgtgagggg tgtcttcctt gatttcaacc
1741 gtgttgacac agagcctgtg cgctttgctg acttcatggt attttaaagt ctccacttta
1801 aaaggccccg tgtaacctct gactaaccga gatgatttat catggagatt aggtctttct
1861 atgcaggctg agcagaagag tttggtctct ttggggtcaa ttactaacca tgggaactgc
1921 ccaaaccatg acctctgaat ggaacggggc ctatatgtcc tcttgattct cctaggtcca
1981 tctccttcct cacaaatgct ggaactgcag caggaggcac gggcctccac gggagagcct
2041 ggaagcaacg cggagtctgc agccgaggcc tgtgtgtctg cctctctcac tgccaccatc
2101 ttcttgttcc ctttggagaa aattgaatca tgcttggttc tgctacccag aactctgcca
2161 tcttctatca gctaagacac ccccaaattt aataagtatc ccttaagcaa ggcagagaag
2221 atgaatgcaa tccttctttc tagagaaagt ggcgtccact taaaccctca cccattctca
2281 tctgtgaaag ttcatctggc tcccccagag ttgttgctaa tccttgtggg ttctttcacc
2341 agcgccccaa ccaccctatc cacacagcta ccctgggatc atgtgacacc agataccaat
2401 aagttcaatt aacccccttg gccacagaga ctgttggata agcaggtggc tttcactctc
2461 gtgtgaggct ccacaataga ataggttaaa cacctgccaa ggcttttaca agcctcagaa
2521 ggaagtaggt acagaagtaa gtgtagcaac tacaagccaa gaaattactg attgagatag
2581 gcaagacaac gaattctcac aaaccaatag tgctgtcacc gagcactgga gaaggaggga
2641 gaagggatga gaggctcaca gatctctggg tcccctggct cctcgagacc agtgtcccca
2701 cgtacctgct cccaccagct ctgccacagc ctccatcagt acttctgctt acaaaaggac
2761 ctgttttttc cctgtttccc agactactct acactctgtg ggcagggtta gttgcttatt
2821 catcttgtct acccagggcc tcctgccagg cttggtgccc aatcggcccc catcagatga
2881 gcacagctga gctcatgctg tttgactcat catagccgcc tgggccctgg cacatgtcct
2941 tcagaatgct gtaggtttac actcacctgg aggacgacct ttcccccact ttggcccatt
3001 tgggtccttg atccagggtg cagccctccg ctccagtttg gagatcaagt ctggcttggc
3061 agcggcaggt cctgttgtaa ggatgaagaa tcatgctgat gtcactgctg tggccaaatc
3121 gagtagcccc ctcaagctag acccagtccc tgaagtacac agaggtgtct ctgggttctg
3181 tccttataaa gtctagtcgg ccaggggcag gctgagcgca aacccagagt gccaaagagg
3241 cagtaagggg aggggcagcc ctcagctagg atagggcttc ctcagatcca tgggccagcc
3301 atacacacca gagggggaag ggtggaaaca ggaagaaaca tagggactaa gcaggagaga
3361 gaggcagggg gaaacagcag ccatatgaga agtggaaggt gccacccaca gctggcccac
3421 gcgtgtgccc ctctgccccc acagctggcc cacgcgtgtg cccctctgcc cccacagctg
3481 gcccacgcgt gtgcccctct gcccccacag ctggcccacg cgtgtgcccc tctgccccca
3541 cagctggccc acgcgtgtgc ccctctgccc ccacagctgg cccatgcgtg tgcccctctg
3601 cccccacagc tggcccatgc gtgtgcccct ctgcccccac agctggcctt tgtgaaggtg
3661 accaactaca tgggtttttg aaaggggcac ttggagggcc ccctgaaata cctaccacct
3721 gcaatggagc ctgaaatctg actaaaggag atttgtgtct ttggattaag cactaacctt
3781 tacttaaaat aggaatattg ttcaggggta tgcagataaa ccatttcctc tattgaaaat
3841 aaaatccatc actatctaca tc
//