LOCUS AK125303 2413 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ43313 fis, clone NT2RI2013357.
ACCESSION AK125303
VERSION AK125303.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2413)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..2413
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2013357"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000045176"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 287..877
/codon_start=1
/protein_id="BAG54180.1"
/translation="MGNKAKIAKCPLRTKTGHILKSTQDTCIGSEKLLQKKPVGSETS
QAKGEKNGMTFSSTKDLCKQCIDKDCLHIQKEISPATPNMQKTRNTVNTSLVGKQKPH
KKHITAENMKSSLVCLTQDQLQQILMTVNQGNRSLSLTENGKEAKSQYSLYLNSISNQ
PKDENIMGLFKKTEMVSSVPAENKSVLNEHQETSKQ"
BASE COUNT 740 a 477 c 538 g 658 t
ORIGIN
1 gagcgttctg tggagagagt gcgaggtcag gccatgaact tgggagatgg tttaaagctt
61 gaaactgaat tactggatgg aaaaaccaag ctaatattgt ctccatatga acataaatca
121 aaaatttctg tgaagtgtct taaagtcttt gcttgtctgt ggatagaacc cagtttggat
181 gatgtaccac tttatccttc aactctaagc ccttgatggc tttacttgca ggtttaatta
241 aggtgaagaa gttgctggag tacccgaaat atcactcaat aaatagatgg gaaataaggc
301 caagattgca aaatgtcctt taagaacaaa aactgggcac attctaaaat caacacaaga
361 tacttgtatt gggagtgaaa aacttttgca aaagaagcca gttggttcag aaacatcaca
421 ggcaaaaggt gaaaaaaatg gaatgacttt ttcatccact aaggatttat gtaaacaatg
481 tatagataaa gactgtcttc atatccagaa agagatttca cctgcaaccc ctaatatgca
541 gaagactaga aacaccgtaa atacatctct agtaggtaaa cagaagcctc acaaaaaaca
601 catcacagct gaaaacatga agagcagttt ggtgtgtcta acacaagacc aactacaaca
661 gattttgatg actgtaaacc aaggaaatag atctctttcc ctgactgaga atggaaagga
721 ggcaaaaagt caatatagtc tatatttaaa cagtatttct aatcagccaa aggatgagaa
781 cattatggga ttattcaaaa aaactgaaat ggtttcatct gtcccagctg aaaataaatc
841 tgtcttaaat gaacatcagg agacatctaa acagtagaga atgaatggaa accagctgat
901 atattcagta ctctggggga aagggaatgt gatagaagtt cgttggaagc aaaaaaagcc
961 cagtggagga aagagctagt tttgcagact gtacaagcat ggtaccagca tctcctcggc
1021 ttctggtgag gcctcaggaa gcttttactc acggtggaag gcaaaggggg agcaggcatt
1081 ttacatggca agagagatgc caggctcttt tacacaacca gctctcatgt gaactgacag
1141 agggagaact caagcatcat cccaagggga tggcaccaag ccattcatga gggattcatc
1201 tcctgtgacg caaacacctc cccttaggcc ccacctccaa cactggggaa tcacatttca
1261 acatgagatt tgaaggggac aaatatccaa actgtatcat caaggtaaaa gaagaatgaa
1321 gagattaaac ttatgttagc agtatcagaa ggtttggcgg tcttgatgtg tgtttatatc
1381 acaatttcat ctttaaatat tttctttcag cacattccac atcaaaatgc aataccaaat
1441 gtctgaaata atatggaact acttggaaat gaccagaata ttttgtgctt taatcatttt
1501 tgttgttgtt gttgttttga gacggagttt cattcttgtt gcccaagctg gagtgcggtg
1561 gcgccgtctc agctcactgc aacctctgct tgctgggttc gggtggttct cctgcctcag
1621 cctcctgagt ggctgggatt acaggcacct gccaccatgc ccaacttact ttttgtatct
1681 ttggaggaga cagggtttca ccatgttggc caggctggtc tcaaactcct ggcctcaggt
1741 gatccgcctg ccttggcctc ccgaagtgct gtgattacag ttgtgagcca ccacacctgg
1801 ccttatgctt taattatttt aaaaatcagt ttactgtttt tttaacttgt ctcagtaaag
1861 tgcctctatt taagtcatgc ttccagtaac attcttggaa tttaaattta tacaacattg
1921 aattttgtat ttaaagaact actgttgatt ggggggttag ttttgactat atctctcaga
1981 cacaggcttt tatgtttttt acttgtcagc aattaagaaa taatagctaa cattcattga
2041 atatttactt tgtgccaagc cctatattga gataggcact attattatcc ccattttata
2101 gaagaaacca aggcttagaa agcttaccag cagttggcca actgcggtgg gctcatgcct
2161 gtaatcctac cacgttggga ggccgaggca ggtggatcac aaggtcggga gatggagacc
2221 atcctggcca acatggtgaa accacatctc cactaaaata caaaaattag ccgggcgtgg
2281 tggtgctgcc tgtggccccg gctactcagg aggctgaggc aggggaatcg cttgaacctg
2341 ggaggcggag attgcagtga gctgagatgg cgccattgca ctccaggctg ctgacagaat
2401 gagactctat att
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