LOCUS AK125302 2299 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ43312 fis, clone NT2RI2012659.
ACCESSION AK125302
VERSION AK125302.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2299)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..2299
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2012659"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000045175"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 100..528
/codon_start=1
/protein_id="BAC86120.1"
/translation="MNVVAQRRGEGWGEMRNRKKIPREIKRPRGAEPGSRWSRQMEQQ
RQRTEPQSGHTARPGSQALWVWWEQMLVWEQQLMAPSTCILVDSRPSTASSAAGLSLE
EQPWSREADEWQPCPVGAPVLLLHPPPGSAHLLRLPPRAS"
BASE COUNT 613 a 566 c 716 g 404 t
ORIGIN
1 agagagagca ggagaatgaa catgggggag acgcagagaa gcacacagag gcatgactga
61 taagggcaga ggaggaaaga aggtctcgga gaccagggca tgaatgtggt tgcacagaga
121 cggggagaag gatggggaga aatgaggaac aggaagaaga ttccgagaga aataaagagg
181 ccgagaggag ctgagccagg cagccggtgg agcaggcaga tggaacaaca gagacaaaga
241 acagagccac agagtgggca cacagcaagg cctggcagcc aagccctgtg ggtttggtgg
301 gagcagatgc ttgtatggga gcagcagctg atggccccca gcacgtgcat ccttgttgac
361 tccaggccca gcacagcctc ctcggcagcc ggccttagcc tggaggagca accctggtcc
421 agagaggcag acgaatggca gccctgcccc gtgggggctc cggttcttct cctccatcct
481 cctccaggct ccgcccacct gctccgtctg cctcccaggg cctcgtgaat cccagggaag
541 ccatgagctg ccacatcacg cccggaagga agcgggctgc gttggagggg aaaagacgca
601 ccagcagcct agaattcgag gcactgcaga gccggaaggg gcctcggcgc tgctccctcc
661 gccccctcat ttcacaaacc ggaaactgag acccaaagca aggaagggac ttggccaagt
721 tgaagagatg aaggcgcaga ggcatgaact cccgtagcca gagagtgtga gctggactct
781 acctggagca gcctcggtgc agagcccatg cccccaacca tgtccgtggt cgaggcccag
841 cccagacagg gcacaaaggg gctgaggaag aggaagtaag atgccagggt tgccatgcag
901 gagggcaacc ccaagtgaga tgggcatcgt ggcctagggc ccaccttccg gaagagctca
961 tcagaacttc ggacacactg catgggccta gacccagggc tcgttgagct cctccctcgg
1021 attggcttcg aattttaggt gaatcctctc tagcagccac attcaccctt gcaagcctgc
1081 actttgtcat cttctgaaaa ggtgcaggaa catgatgccc agctgactcc cagggagcgg
1141 gagccccaga ggccagcgga gcacagccgc agcaggcaga tctgttggga aaccacggga
1201 gtataggagc agggctgggg gcacctgagg gactgtggag cctgaattat tgaaacgctt
1261 agagtaaagc agatttagat ttcacaaaga cagaggtgaa ggtgggtgaa gacggaggga
1321 aggatttaaa gggaaagaga aagaggaaag ataagaatga gggagaaaga ggaagagaga
1381 aagaaaaaga gaaatagagt tatcgaagat gactgcaagt ctggcctctc tcaacccaag
1441 gagccaagga ggggaaggcc ggcctgagca gcaacgtaga caagagctca ggagggcagg
1501 gacagagccc tggcttcagc aagagcgtgg ggaacagagg gcaagggggt ccatgcagcc
1561 caggatggca gggtgctcat acacacagcc ttggtcctgt cccctctccc attgccgctt
1621 ttgagctcac cttgtttaag gccaaagcac acaccatcct cccacagagc cactaatgca
1681 attaacaaaa cacattcata aatcttgcat ggcgtccgga gctgtctcct ctccctaagc
1741 acaaaggcca gctccagaga tgctgtgcag agagctgtct gacctctcca gcgtcagggc
1801 cccctgaaaa gtgcagcata acccttagta ggacagaaag accattgtct tctgccaacc
1861 attacaaagt ggagtggggc tggggttagg aaacacagac aaatcagaag aggctgagaa
1921 ggcggagatg aaagtgcata ggtgtttgca gggagctggg gaatagttcg tgccttccaa
1981 gctcaacaag agagcattca gcagagacca gagaatcacc acttttggag ggtgatttta
2041 ccttgtgaga acagagcaca gggtgtgtgg gacccagggc atcttcggca atgcgggcgt
2101 gacttggcgg gatgtcggtg tttgtgtttg catgtgtgtg tgcgtgcgtg catgctcagt
2161 ctggaaaact ggtgactcac taaaacaccg agctttctga tctgaagagc ttatattcgg
2221 ctatttttag ctcgtcttgt ctcttggccc tttttttccc cttgtctgtt ttcaaatgta
2281 gaggagtctg gagaccatg
//