LOCUS AK125300 2150 bp mRNA linear HUM 12-JUL-2008
DEFINITION Homo sapiens cDNA FLJ43310 fis, clone NT2RI2008724, highly similar
to Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2 (EC
2.4.1.174).
ACCESSION AK125300
VERSION AK125300.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2150)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..2150
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2008724"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000045173"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 289..1917
/codon_start=1
/note="highly similar to Chondroitin
beta-1,4-N-acetylgalactosaminyltransferase 2 (EC
2.4.1.174)"
/protein_id="BAG54179.1"
/translation="MPRRGLILHTRTHWLLLGLALLCSLVLFMYLLECAPQTDGNASL
PGVVGENYGKEYYQALLQEQEEHYQTRATSLKRQIAQLKQELQEMSEKMRSLQERRNV
GANGIGYQSNKEQAPSDLLEFLHSQIDKAEVSIGAKLPSEYGVIPFESFTLMKVFQLE
MGLTRHPEEKPVRKDKRDELVEVIEAGLEVINNPDEDDEQEDEEGPLGEKLIFNENDF
VEGYYRTERDKGTQYELFFKKADLTEYRHVTLFRPFGPLMKVKSEMIDITRSIINIIV
PLAERTEAFVQFMQNFRDVCIHQDKKIHLTVVYFGKEGLSKVKSILESVTSESNFHNY
TLVSLNEEFNRGRGLNVGARAWDKGEVLMFFCDVDIYFSAEFLNSCRLNAEPGKKVFY
PVVFSLYNPAIVYANQEVPPPVEQQLVHKKDSGFWRDFGFGMTCQYRSDFLTIGGFDM
EVKGWGGEDVHLYRKYLHGDLIVIRTPVPGLFHLWHEKRCADELTPEQYRMCIQSKAM
NEASHSHLGMLVFREEIETHLHKQAYRTNSEAVG"
BASE COUNT 657 a 396 c 482 g 615 t
ORIGIN
1 gcaggaggcg gtggagcgca gagcgggcga gcgcgaaaaa tcactaccaa tataatggat
61 tttatatatc agattgcttt attctggata tcatggtaac aatacagaaa gtatacataa
121 tttcccattt ctgcaagtag tcatgactgc tgaagaaaga aaaacttaaa gctacggcag
181 aattatttta tggaaattct gattttgttt ttaatttttg ataacttttt actaaaggta
241 tgaacacaca aagagcttat tttgttaggc aaatacacat taataagaat gcctagaaga
301 ggactgattc ttcacacccg gacccactgg ttgctgttgg gccttgcttt gctctgcagt
361 ttggtattat ttatgtacct cctggaatgt gccccccaga ctgatggaaa tgcatctctt
421 cctggtgttg ttggggaaaa ttatggtaaa gagtattatc aagccctcct acaggaacaa
481 gaagaacatt atcagaccag ggcaaccagt ctgaaacgcc aaattgccca actaaaacaa
541 gaattacaag aaatgagtga gaagatgcgg tcactgcaag aaagaaggaa tgtaggggct
601 aatggcatag gctatcagag caacaaagag caagcaccta gtgatctttt agagtttctt
661 cattcccaaa ttgacaaagc tgaagttagc ataggggcca aactacccag tgagtatggg
721 gtcattccct ttgaaagttt taccttaatg aaagtatttc aattggaaat gggtctcact
781 cgccatcctg aagaaaagcc agttagaaaa gacaaacgag atgaattggt ggaagttatt
841 gaagcgggct tggaggtcat taataatcct gatgaagatg atgaacaaga agatgaggag
901 ggtccccttg gagagaaact gatatttaat gaaaatgact tcgtagaagg ttattatcgc
961 actgagagag ataagggcac acagtatgaa ctctttttta agaaagcaga ccttacggaa
1021 tatagacatg tgaccctctt ccgccctttt ggacctctca tgaaagtgaa gagtgagatg
1081 attgacatca ctagatcaat tattaatatc attgtgccac ttgctgaaag aactgaagca
1141 tttgtacaat ttatgcagaa cttcagggat gtttgtattc atcaagacaa gaagattcat
1201 ctcacagtgg tgtattttgg taaagaagga ctgtctaaag tcaagtctat cctagaatct
1261 gtcaccagtg agtctaattt tcacaattac accttggtct cattgaatga agaatttaat
1321 cgtggacgag gactaaatgt gggtgcccga gcttgggaca agggagaggt cttgatgttt
1381 ttctgtgatg ttgatatcta tttctcagcc gaattcctta acagctgccg gttaaatgct
1441 gagccaggta agaaggtgtt ttaccctgtg gtgttcagtc tttacaatcc tgccattgtt
1501 tatgccaacc aggaagtgcc accacctgtg gagcagcagc tggttcacaa aaaggattct
1561 ggcttttggc gagattttgg ctttggaatg acttgtcagt atcgttcaga tttcctgacc
1621 attggtggat ttgacatgga agtgaaaggt tggggtggag aagatgttca tctttatcga
1681 aaatacttac atggtgacct cattgtgatt cggactccgg ttcctggtct tttccacctc
1741 tggcacgaaa agcgctgtgc tgatgagctg acccccgagc agtaccgcat gtgcatccag
1801 tctaaagcca tgaatgaggc ctctcactcc cacctgggaa tgctggtctt cagggaggaa
1861 atagagacgc atcttcataa acaggcatac aggacaaaca gtgaagctgt tggttgaaat
1921 cataattaat gcgttactgt atgaaccaca aaacagcact atttatttag ccttacttct
1981 acttccagat gcagtgcctc ttttggagaa gacatgttta tttttcatgt tctttctgac
2041 attactttag caattcaact tgatgtgaga agaaaaaaca aatgtttcaa cacaaaatct
2101 ctattttgtg agaatactgc actatggaat aattgacaaa ttgaaatctc
//