LOCUS AK123842 4047 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ41848 fis, clone NT2RI3003382.
ACCESSION AK123842
VERSION AK123842.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4047)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Suzuki,O., Sasaki,N., Aotsuka,S., Shoji,T., Ichihara,T.,
Shiohata,N., Matsumoto,K., Hirano,M., Sano,S., Nomura,R.,
Yoshikawa,Y., Matsumura,Y., Moriya,S., Chiba,E., Momiyama,H.,
Onogawa,S., Kaeriyama,S., Satoh,N., Matsunawa,H., Takahashi,E.,
Kataoka,R., Kuga,N., Kuroda,A., Satoh,I., Kamata,K., Takami,S.,
Terashima,Y., Watanabe,M., Sugiyama,T., Irie,R., Otsuki,T.,
Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..4047
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3003382"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000043715"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 600..2642
/codon_start=1
/protein_id="BAG53968.1"
/translation="MGCGTKEPKITQLCLAAIQRLMSHEVVSETAAGNIINMLWQLME
NSLEELKLLQTVLVLLTTNTVVHDEALSKAIVLCFRLHFTKDNITNNTAAATVRQVVT
VVFERMVAEDERHRDIIEQPVLVQGNSNRRSVSTLKPCAKDAYMLFQDLCQLVNADAP
YWLVGMTEMTRTFGLELLESVLNDFPQVFLQHQEFSFLLKERVCPLVIKLFSPNIKFR
QGSSTSSSPAPVEKPYFPICMRLLRVVSVLIKQFYSLLVTECEIFLSLLVKFLDADKP
QWLRAVAVESIHRFRVQPQLLRSFCQSYDMKQHSTKVFRDIVNALGSFIQSLFLVPPT
GNPATSNQAGNNNLGGSVSAPANSGMVGIGGGVTLLPAFEYRGTWIPILTITVQGSAK
ATYLEMLDKVEPPTIPEGYAMSVAFHCLLDLVRGITSMIEGELGELETECQTTTEEGS
SPTQSTEQQDLQSTSDQMDKEIVSRAVWEEMVNACWCGLLAALSLLLDASTDEAATEN
ILKAELTMAALCGRLGLVTSRDAFITAICKGSLPPHYALTVLNTTTAATLSNKSYSVQ
GQSVMMISPSSESHQQVVAVGQPLAVQPQGTVMLTSKNIQCMRTLLNLAHCHGAVLGT
SWQLVLATLQHLVWILGLKPSSGGALKPGRAVEGPSTVPFKDFMQPPASRVQNGES"
BASE COUNT 1147 a 782 c 888 g 1230 t
ORIGIN
1 gactcggcta atggcgtcgg cgagtcttag gggcctgggg agctggcgct gaagcttctt
61 gccaggttgg ctggtgacac ccggtgtggc tgggccccgc ggcagcggag ggacctgccc
121 gccttgtggg tttctcggcc agagtcggcg gagcctagcg ggacggtgcg actgcggggg
181 gcgcctccga gaaaagccag aggtgttgcg gggaagctgc tgggggacgc tcgagcaggc
241 tccgggttcg cagcccaggg cccaagaagc gggctgctga aggaccagag acaccgggag
301 ggagctgcct gtggccctaa ggagctgacc gtgccagagc ttgtttgtac ctctcggaaa
361 ttggctggga ccttggagga tcatgtccgg caccagcagc cccgaggcgg tgaagaagct
421 gctggagaat atgcagagcg acttgcgcgc cttgtcactg gagtgcaaga agaaattccc
481 acctgtcaaa gaggctgctg aatcaggaat aataaaagtt aaaacaattg ctgcacgaaa
541 cactgaaatt ttggcagcac tgaaagagaa cagctcagag gttgtacagc ctttttttaa
601 tgggttgtgg aaccaaggaa ccgaagatca ctcagctatg tttggctgct attcagagac
661 tcatgtcaca tgaagtcgtg tctgagactg cagctggaaa tataattaac atgctttggc
721 agctaatgga gaatagtctt gaagaactta agctacttca aacagttctt gttcttttaa
781 caaccaatac agtagttcat gatgaggcac tttctaaggc aatcgttctt tgttttcgac
841 tacacttcac aaaagataat attacaaata atacagctgc tgctacagtg cgacaagttg
901 ttactgttgt ttttgagagg atggttgctg aagatgaacg acacagagat attatagaac
961 aaccagtact ggtacaagga aatagtaaca gaagatctgt cagtaccctc aaaccttgtg
1021 ctaaagatgc atatatgctt ttccaggatc tttgtcagtt ggttaatgct gatgctcctt
1081 attggctagt gggcatgaca gaaatgactc ggacgtttgg cctcgaatta cttgagtcag
1141 tcctcaatga ttttccgcag gtctttttac aacaccaaga atttagtttc ctcctcaaag
1201 aaagggtatg tcctcttgtg ataaagctct tttctccaaa tataaagttc agacaaggtt
1261 ccagcacctc atcttctcca gcaccagttg aaaaaccata ttttcctatc tgcatgcgtt
1321 tgctgagagt agtatctgtt ctgattaagc agttttacag tcttttggta actgaatgtg
1381 agatatttct gtcacttctg gtgaaatttc tggatgcaga taaaccacag tggctacgag
1441 ctgttgcggt ggaatcaata cacagattcc gtgtgcagcc tcaactatta aggtcatttt
1501 gtcagtccta tgatatgaaa cagcattcta ccaaggtttt tcgtgatatt gtaaatgcac
1561 tgggatcttt tatacagtcc ttgtttcttg tcccccctac tggaaatcct gcaacaagca
1621 accaagctgg aaacaataat ttaggtggct cagtctcagc accagctaac tcaggaatgg
1681 tggggattgg tggaggtgtt actttgctac cagcatttga atatagggga acctggatac
1741 ctattctgac aatcacagtt caaggcagtg ctaaagccac ctacttagaa atgttggaca
1801 aagttgagcc tccaactata cctgaaggtt acgccatgtc tgtggcattc cattgtttgc
1861 tagaccttgt tcgtggaatc acaagtatga ttgaaggaga gctaggagag cttgaaacag
1921 aatgtcaaac caccactgaa gaaggttctt caccaacaca gtcgacagaa cagcaggatt
1981 tacagtcaac atcagaccaa atggataagg aaattgttag tagggctgtt tgggaagaaa
2041 tggtgaatgc ctgctggtgt ggtcttcttg ctgcactctc actccttctt gatgccagca
2101 cagatgaagc tgccactgag aatattttaa aagctgaact gactatggct gctctttgtg
2161 gaagactggg ccttgtaact tcaagagatg cctttataac tgcaatatgc aaaggttccc
2221 tgcctcccca ttatgctctt actgtattga ataccaccac tgcagctaca ctttccaaca
2281 aatcatattc cgttcagggc caaagtgtta tgatgataag tccatcaagt gaatctcacc
2341 aacaagttgt ggcagtgggt caacctttag cagtccagcc tcaagggaca gtaatgctga
2401 cttccaaaaa tatccagtgt atgaggactt tacttaactt ggcgcattgc catggggctg
2461 ttcttggaac atcatggcaa cttgtcttgg caactcttca gcatcttgtg tggattctgg
2521 gattaaagcc tagtagtggc ggtgccttga aacctgggag agctgtagaa ggacccagta
2581 cagttccttt taaggatttc atgcagccac cagcatccag agttcaaaat ggagaatctt
2641 gaccggctac aatatatttg aaagcaggaa gatagtctaa aaaatgtttg ctcctaattg
2701 agtcttctgt gagaaggaca tttcttactg cagataattc ttggcagctg ttgttggcct
2761 cctttaaatt ctacttacct gagttcagta attcatatta caggcttgca catcaacaaa
2821 ggctcctgaa tgaacagcag tgtaaggctt taataaatta aactgatggg agggataatt
2881 aacactacag tatacatgct accatatctc cagttggtga tttaaagtga gcttatgtac
2941 agtttgtggt gtatgtgtta atgatgtact ttttaaaaag aaagaagaga tatttcaatt
3001 cagtcagatt tattagtctg gtgtttttgc accctttttc aagtacaaaa tcgtactaga
3061 attttatgca agatggtact gtaacattcc atattatcta tgaccagcct ttgttaacaa
3121 agggaactga tatacttgtg tgtataataa atggtacagt tctgtataaa atagtgcatt
3181 tatttaaatt ttaaaagtat tgataatgtt aaatgcttaa agctctattt attattaata
3241 caaaattgtt tgcttacatt tttacttata atttgccttc atatgtggcg gataagctca
3301 ccatatgatc atgcagttag cttcatgctt attttaaatg tattattagt gaccattaaa
3361 catctgacca gtaaggtcat gtgaacacag cagcaaatag tttatgattt gctgattttg
3421 gagctttgaa atataggttc ttaatacatt gatacatatt gtagcactat gacttcatca
3481 tacctcattt ctttaaacag ctctccaagc tttcactgaa gtctgtctgt tttttatatt
3541 ggctgtctgg attttaaaga cttttcatat tttatatttc tactgatttt gtttccccta
3601 acaacatttg tcactgtctt tgaattatga cccaggcaag atgatttcag attttctaaa
3661 atcttgcctg tgaggttttg ttcataacag tgcttcattt tgtaatgtct tctcaagaaa
3721 aatacctatg ttaactcaca agtataaaat atgtgtgtat tataaaacaa tgaaaagtgt
3781 atttttggag atagtcaagc atttagaagt gcagtgaact tgctgtcacg gagtaaaatg
3841 ctaattatgt ttcactttcc tagcctagtg aaaaagaaaa gtgctcttga gtacaatacc
3901 ttaattattt cttaaaatac tgactttgac ctagctcact gtatttttta tttaatggat
3961 tatggattac agtatttttc ttctgagtta aattttcata atttatgtga agacacaaag
4021 atgtttaaaa caatgattat tcataag
//