LOCUS AK122748 2262 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ16273 fis, clone NT2RI2010508, highly similar
to Homo sapiens diacylglycerol lipase beta.
ACCESSION AK122748
VERSION AK122748.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2262)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (15-JUL-2003) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Tashiro,H., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2003)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: Reverse Proteomics Research
Institute, HRI and RAB.
FEATURES Location/Qualifiers
source 1..2262
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2010508"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000042621"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 380..1555
/codon_start=1
/note="highly similar to Homo sapiens diacylglycerol
lipase beta"
/protein_id="BAG53702.1"
/translation="MQFAAAAYGWPLYIYRNPLTGLCRIGGDCCRSRTTDYDLVGGDQ
LNCHFGSILHTTGLQYRDFIHVSFHDKVYELPFLVALDHRKESVVVAVRGTMSLQDVL
TDLSAESEVLDVECEVQDRLAHKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVG
HSLGGGAAALLATMLRAAYPQVRCYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPR
LSVTNLEDLKRRILRVVAHCNKPKYKILLHGLWYELFGGNPNNLPTELDGGDQEVLTQ
PLLGEQSLLTRWSPAYSFSSDSPLDSSPKYPPLYPPGRIIHLQEEGASGRFGCCSAAH
YSAKWSHEAEFSKILIGPKMLTDHMPDILMRALDSVVSDRAACVSCPAQGVSSVDVA"
BASE COUNT 470 a 629 c 665 g 498 t
ORIGIN
1 aatttggtgg cggcgtccgg agggtgctgg tttgttctcg gtgaacggcg cgcggggtct
61 ctcctgagtg cgagctacgg gaccttcgcc atgccgggga tggtactctt cggccggcgc
121 tgggccatcg ccagcgacga cttggtcttc ccagggttct tcgagctggt cgtgcgagtg
181 ctgtggtgga ttggcattct gacgttgtat ctcatgcaca gaggaaagct ggactgtgct
241 ggtggagcct tgctcagcag ttacttgatc gtcctcatga ttctcctggc agttgtcata
301 tgtactgtgt cagccatcat gtgtgtcagc atgagaggaa gctgatctgg atgcagaatt
361 agaaaactgc catcattaca tgcagtttgc agcagcggcc tatgggtggc ccctctacat
421 ctacagaaac cccctcacgg ggctgtgcag gattggtggt gactgctgca gaagcagaac
481 cacagactat gacttggtcg gaggcgatca gctcaactgt cacttcggct ccatcctgca
541 caccacaggg ctgcagtaca gggacttcat ccacgtcagc ttccatgaca aggtttacga
601 gctgccgttt ttagtggctc tggatcacag gaaagagtct gttgtggtcg ctgtgagggg
661 gaccatgtct ctgcaggatg tccttacgga cctgtcagcg gagagtgagg tgctggacgt
721 ggagtgtgag gtgcaggacc gcctggcaca caagggtatt tctcaagctg ccagatacgt
781 ttaccaacga ctcatcaacg acgggatttt gagccaagcc ttcagcattg ctcctgagta
841 ccggctggtc atagtgggcc acagcctcgg gggcggggcg gccgccctgc tggccaccat
901 gctcagagcc gcctacccgc aggtcaggtg ctacgccttc tccccacccc gggggctgtg
961 gagcaaagct ctgcaggaat attctcagag cttcatcgtg tcactcgtcc tggggaagga
1021 tgtgattccc aggctcagtg tgaccaactt ggaagatctg aagagaagaa tcttgcgagt
1081 ggtcgcgcac tgcaataaac ccaagtacaa gatcttgctg cacggtttgt ggtacgaact
1141 gtttggagga aaccccaaca acttgcccac ggagctggac gggggcgacc aggaagtcct
1201 gacacagcct cttctggggg agcagagcct actgacgcgc tggtccccgg cctacagctt
1261 ctccagcgac tccccactgg actcttctcc caagtacccc cctctctacc ctcccggcag
1321 gatcatccac ctgcaggagg agggcgcctc ggggcggttt ggctgctgct ctgctgctca
1381 ctatagcgcc aagtggtcac acgaagcgga attcagcaaa atactcatag gtccgaagat
1441 gctcaccgac cacatgccag acatcctgat gcgggccttg gacagcgtgg tctccgacag
1501 agcggcctgc gtctcctgtc cagcacaagg ggtctccagt gtggacgtgg cctgaccagg
1561 gccactggaa actgtcccag gaacgatgga ctcacgcttt tgtccttaaa ctgacttacc
1621 atccgaggag ttcccatgac gccaaaacag cgaatgtcca tcaacaggaa tcggatggga
1681 acagaattcc atggtctcaa tgacttaagt ttatgggaag tcattgtggc cataatggta
1741 gcagaagtag tgagcacgct caggtgatag gacgactcct gagacccagc gaccgtggag
1801 acagcctcgg gaagccctgg cccgtggatg gatcccttgg ctgtctgagg actgctccag
1861 aagtgcggga atccagggcc cacccagaag accgtgaaca gttccttagc ctcccacccc
1921 caaggcagct cttttcatcc aactcagttt acaggcgtgg tttgtttttc aaactgggct
1981 tcctggatgt acaaatggaa ctgtggtgag ggtgcgggct ggggttttct cctgggcgtc
2041 accaagggca gccctgggct ctggctgggg atgaagacga aacccgatcg ggaaagtaag
2101 tggagccccc ggccccgccg agccacagcc ccccaactgc ctattcccac tgcccagttg
2161 tttgtccaca tcaggagttg ctgattgaat tcttgctact cttctggctc tggggtcggc
2221 cagtggattc aggagttgaa acaataaagc gcgcgtcacc at
//