LOCUS AK096327 2008 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ39008 fis, clone NT2RI2025255.
ACCESSION AK096327
VERSION AK096327.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2008)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Kanehori,K., Ishibashi,T., Chiba,Y., Fujimori,K., Hiraoka,S.,
Tanai,H., Watanabe,S., Ishida,S., Ono,Y., Hotuta,T., Watanabe,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H.,
Kanda,K., Wagatsuma,M., Takahashi-Fujii,A., Oshima,A.,
Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S., Nagahari,K.,
Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..2008
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2025255"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000021182"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 332 a 646 c 637 g 393 t
ORIGIN
1 gtcgctttaa ggcggaggcc cgggaggggg ccgacccggc tcgccagctc cacgctcggc
61 tccagactcc ggcatttcct ccccgctagc tggcgcggcc tcgcctcccc ctcggaagag
121 gaaactcccg gggtccgagt aacagggtca ggcgcggaga ggagcggcgg gagagccagg
181 agggccgccc aggctatgtc ctcctgcgct gtcgccacgc tgtccggcag cgcttcctgc
241 ccgggtctcc ccgtctggag ggtcacgccg ccttctcctc gagacacttc cgagagccgg
301 gcctcagcat cctgctggag agttactacg agcatgaggt gcgcctgtct ccgcacgtgt
361 tgggccacag caaggcgcac gtgagccgga tcgtgggcga gctggtgcgg gctggccgcg
421 cccgggggtc ccccggtctc attcctgggg gagcgctggc cttggccttc cgcggagact
481 tcatccaggt gggcagcgcc tacgagcaac ataaaatccg ccggcccgac agcttcgacg
541 tgctggtgcc actgcgcctc ccgccgcttg tggcgctgga gccacggagc ctgggcgagg
601 agccagcgct ggccccggcc ttccgcggct gcttcttgtg cgccctcaag gcaccaccct
661 caccatcggg ggcctcgggg ggccactggc ttcgggactg caaacccttt gctgatgcct
721 tctgcgtgga tgtgcgcggg cggcgtcacc tctctgctac tctggtgctg cgctggttcc
781 agtcgcatct gcagcgctcc ttggccactg tgcgttacag cctggagggg cgctgtcggg
841 tcaccttgac cccaggtggc ctggaacagc cccccacctt acacatcttg ccctgccgca
901 ctgactacgg ctgctgccgc ctttctatgg ctgtgcgtct catccccgct gtccatctgg
961 gagatggggt cttccttgtg gcgccaccac cgccaccctt gcccagcgcg cccctgttgg
1021 agctccctga gggcctgcgt gcggaggcac tgtggggtgt gaacacagca cgccaggagc
1081 agaagctgct gagttggctg caggaacggg cagctccagg tgcctgctac ctcaagtgcc
1141 tgcagttgct taaggctctg cgcgatctgg gggcccgtgg gctggactca gcggccgcca
1201 cccagtgggg acgcatccta tcctcatatg tgctcaagac agtgctgctg gcagtgctgc
1261 tgcgcaaggg ggcccctggg caaggctggg acgaggagca cctgggaagg tgtttggagg
1321 agttggtgca gttccttagg gactgcctgc tgcgacgcca tacgctcttc cactgcgtcc
1381 tgggccctgg tggggcggct gccgaggtgg gtcccctgcc caaggcactg agggaagccg
1441 ccccagttga cctcctggcc gctttcgacg ggcacgcccg ggaacttgca gcagcgcggt
1501 tgctgtccac gtggcaaagg ctgccccagc ttctccgggc ctacgggggt ccccgctacc
1561 ttgccaggtg ccccccaccc cggagtcagc gcacccaggg cttccttgaa ggtgaaccgt
1621 aaaccctgac agcaccccca cctgaccaaa tgctcctaaa gcctttccca ctgggtgggg
1681 gtgggaatgg cggtgaagcc agttaaatgc aagattgcag aaggcattgg aaaatttggt
1741 ggctgccaca agctttagtg gcttaaatat caccttctcg cttcacagtc cagtataata
1801 tgacatcttc acacccacta gagtgtcctg ggcaaaccat gggaagacat ccaacaggag
1861 acccaagaat tggttcaaat attgttctgt gtagacggat tctgtagaag gatgtggctt
1921 ttagagaagt ccagtagaag aagcaagaac tagctgcagg gaaagttcct tctgtcggtt
1981 tttagacaca gatctctctg cccaaatt
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