LOCUS AK096313 2778 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ38994 fis, clone NT2RI2009259, highly similar
to Cysteinyl-tRNA synthetase (EC 6.1.1.16).
ACCESSION AK096313
VERSION AK096313.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2778)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..2778
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2009259"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000021168"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 378..2594
/codon_start=1
/note="highly similar to Cysteinyl-tRNA synthetase (EC
6.1.1.16)"
/protein_id="BAG53256.1"
/translation="MQTPPLQQPHQEQVFLAFLVIVIPSFLTKEVFIPQDGKKVTWYC
CGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITDIDDKIIKRARQNHLF
EQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQLATEPLEKAV
QSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDME
ALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFASSEKHSYGKL
VPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGW
HIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTI
AGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFLN
EFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVMEEM
RALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGT
SLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEG
LPTVVKLVDRNTLLKEREEKRRVEEEKRKKKEEAARRKQEQEAAKLAKMKIPPSEMFL
SETDKYSKFDENGLPTHDMEGKELSKGQAKKLKKLFEAQEKLYKEYLQMAQNGSFQ"
BASE COUNT 721 a 700 c 761 g 596 t
ORIGIN
1 agattctagg aagtgtctgt agccgcagct gcgggtccgg gattcccagc catggcagat
61 tcctccgggc agcaggctcc tgactacagg tccattctga gcattagtga cgaggcagcc
121 agggcacaag ccctgaacga gcacctcagc acgcgtagct atgtccaggg gtactcactg
181 tcccaggcag acgtggacgc gttcaggcag ctctcggccc cgcccgctga cccccagctc
241 ttccacgtgg ctcggtggtt caggcacata gaagcgctcc tgggtagccc ctgtggcaaa
301 ggccagccct gcaggctcca agcaagcaaa ggccggcgtg tgcagcccca gtggtcccct
361 cctactggga cccagccatg cagactccac ctttacaaca gcctcaccag gaacaagtgt
421 ttcttgcatt tcttgtgatc gtcattcctt cattcctaac aaaggaagtg ttcatacctc
481 aagatgggaa aaaggtgacg tggtattgct gtgggccaac cgtctatgac gcatctcaca
541 tggggcacgc caggtcctac atctcttttg atatcttgag aagagtgttg aaggattact
601 tcaaatttga tgtcttttat tgcatgaaca ttacggatat tgatgacaag atcatcaaga
661 gggcccggca gaaccacctg ttcgagcagt atcgggagaa gaggcctgaa gcggcacagc
721 tcttggagga tgttcaggcc gccctgaagc cattttcagt aaaattaaat gagaccacgg
781 atcccgataa aaagcagatg ctcgaacgga ttcagcacgc agtgcagctt gccacagagc
841 cacttgagaa agctgtgcag tccagactca cgggagagga agtcaacagc tgtgtggagg
901 tgttgctgga agaagccaag gatttgctct ctgactggct ggattctaca cttggctgtg
961 atgtcactga caattccatc ttctccaagc tgcccaagtt ctgggagggg gacttccaca
1021 gagacatgga agctctgaat gttctccctc cagatgtctt aacccgggtt agtgagtatg
1081 tgccagaaat tgtgaacttt gtccagaaga ttgtggacaa cggttacggc tatgtctcca
1141 atgggtctgt ctactttgat acagcgaagt ttgcttctag cgagaagcac tcctatggga
1201 agctggtgcc tgaggccgtt ggagatcaga aagcccttca agaaggggaa ggtgacctga
1261 gcatctctgc agaccgcctg agtgagaagc gctctcccaa cgactttgcc ttatggaagg
1321 cctctaagcc cggagaaccg tcctggccgt gcccttgggg aaagggtcgt ccgggctggc
1381 atatcgagtg ctcggccatg gcaggcaccc tcctaggggc ttcgatggac attcacggag
1441 gtgggttcga cctccggttc ccccaccatg acaatgagct ggcacagtcg gaggcctact
1501 ttgaaaacga ctgctgggtc aggtacttcc tgcacacagg ccacctgacc attgcaggct
1561 gcaaaatgtc aaagtcacta aaaaacttca tcaccattaa agatgccttg aaaaagcact
1621 cagcacggca gttgcggctg gccttcctca tgcactcgtg gaaggacacc ctggactact
1681 ccagcaacac catggagtca gcgcttcaat atgagaagtt cttgaatgag tttttcttaa
1741 atgtgaaaga tatccttcgc gctcctgttg acatcactgg tcagtttgag aagtggggag
1801 aagaagaagc agaactgaat aagaactttt atgacaagaa gacagcaatt cacaaagccc
1861 tctgtgacaa tgttgacacc cgcaccgtca tggaagagat gcgggccttg gtcagtcagt
1921 gcaacctcta tatggcagcc cggaaagccg tgaggaagag gcccaaccag gctctgctgg
1981 agaacatcgc cctgtacctc acccatatgc tgaagatctt tggggccgta gaagaggaca
2041 gctccctggg attcccggtc ggagggcctg gaaccagcct cagtctcgag gccacagtca
2101 tgccctacct tcaggtgtta tcagaattcc gagaaggagt gcggaagatt gcccgagagc
2161 aaaaagtccc tgagattctg cagctcagcg atgccctgcg ggacaacatc ctgcccgagc
2221 ttggggtgcg gtttgaagac cacgaaggac tgcccacagt ggtgaaactg gtagacagaa
2281 acaccttatt aaaagagaga gaagaaaaga gacgggttga agaggagaag aggaagaaga
2341 aagaggaggc ggcccggagg aaacaggaac aagaagcagc aaagctggcc aagatgaaga
2401 ttccccccag tgagatgttc ttgtcagaaa ccgacaaata ctccaagttt gatgaaaatg
2461 gtctgcccac acatgacatg gagggcaaag agctcagcaa agggcaagcc aagaagctga
2521 agaagctctt cgaggctcag gagaagctct acaaggaata tctgcagatg gcccagaatg
2581 gaagcttcca gtgagggggc acaggactga ctttttaaac cattgtggac tagtggctgc
2641 tgtctgcctc agtgacaatg tcccagcgct cctatcatgt ttacagtcac ccttgggtcc
2701 taaattaaga gttgtgttca tgtaggttcg tgtcgtcgtt ggctctgaga cattgataat
2761 aaatttttct caacagtg
//