LOCUS AK096299 1884 bp mRNA linear HUM 11-JUL-2008
DEFINITION Homo sapiens cDNA FLJ38980 fis, clone NT2RI2004884, highly similar
to Guanine nucleotide-binding protein G(i), alpha-2 subunit.
ACCESSION AK096299
VERSION AK096299.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1884)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..1884
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2004884"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000021154"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 330..1349
/codon_start=1
/note="highly similar to Guanine nucleotide-binding
protein G(i), alpha-2 subunit"
/protein_id="BAG53252.1"
/translation="MTEGVKTLGWTKQKGGCHWGRSEGAGESGKSTIVKQMKIIHEDG
YSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQG
VLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVL
RTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLV
LAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYT
GANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLK
DCGLF"
BASE COUNT 423 a 568 c 519 g 374 t
ORIGIN
1 aatagagacg gggtttcacc gtgttagcca ggatggtctc gatctcctga cctcgtgatc
61 cgcccgcctt ggcctcccag agtgctggga tgacaggcgt gagcctctga gagcaaattt
121 tacccagttc ctctctatcc tcccgatgac tctgaaatca aactcagcaa gtcatccgag
181 cccccacggc gcagcggccc taggctggca tgggcgatgc ggagctactc cgccctcagg
241 aagcaggcga ggtgcccagc gggagaatca cccacatcac cgtctaatct ctgctgtgaa
301 gtggaagcgc gagaaggagg gagcgtctca tgacggaggg tgtgaagacg ctaggctgga
361 cgaagcagaa aggcgggtgt cactggggac gttctgaggg tgctggggag tcagggaaga
421 gcaccatcgt caagcagatg aagatcatcc acgaggatgg ctactccgag gaggaatgcc
481 ggcagtaccg ggcggttgtc tacagcaaca ccatccagtc catcatggcc attgtcaaag
541 ccatgggcaa cctgcagatc gactttgccg acccctccag agcggacgac gccaggcagc
601 tatttgcact gtcctgcacc gccgaggagc aaggcgtgct ccctgatgac ctgtccggcg
661 tcatccggag gctctgggct gaccatggtg tgcaggcctg ctttggccgc tcaagggaat
721 accagctcaa cgactcagct gcctactacc tgaacgacct ggagcgtatt gcacagagtg
781 actacatccc cacacagcaa gatgtgctac ggacccgcgt aaagaccacg gggatcgtgg
841 agacacactt caccttcaag gacctacact tcaagatgtt tgatgtgggt ggtcagcggt
901 ctgagcggaa gaagtggatc cactgctttg agggcgtcac agccatcatc ttctgcgtag
961 ccttgagcgc ctatgacttg gtgctagctg aggacgagga gatgaaccgc atgcatgaga
1021 gcatgaagct attcgatagc atctgcaaca acaagtggtt cacagacacg tccatcatcc
1081 tcttcctcaa caagaaggac ctgtttgagg agaagatcac acacagtccc ctgaccatct
1141 gcttccctga gtacacaggg gccaacaaat atgatgaggc agccagctac atccagagta
1201 agtttgagga cctgaataag cgcaaagaca ccaaggagat ctacacgcac ttcacgtgcg
1261 ccaccgacac caagaacgtg cagttcgtgt ttgacgccgt caccgatgtc atcatcaaga
1321 acaacctgaa ggactgcggc ctcttctgag gggcagcggg gcctggcggg atgggccacc
1381 gccgactttg taccccccaa cccctgagga agatgggggc aagaagatca cgctccccgc
1441 ctgttccccc gccgcttttc tcctctttcc tctctttgtt ctcagctccc cctgtcccct
1501 cagctccaga cgtaggggag gggttgccac aggcctccct gtttgaagcc tgcccttgtc
1561 tgagatgctg gtaatggcca tggtaccccc ttctgggcat ctgttctggt ttttaaccat
1621 tgtcttgttc tgtgatgagg ggaggggggc acatgctgag tctcccaagg ctgcgtctgg
1681 aggggcccct gcttctccag cctggacccc cagctttgcc caacaccagc ccctgcccca
1741 gcccaagtcc aaatgtttac agggagcctc ctgcccagtc ccccaacccc agccgctcgg
1801 aggccccaaa ggaaaaagca caagaagcgt gagacgccac cattcctgga aaccacagtc
1861 cacctgctca ttctcgtagc tttt
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