LOCUS AK096287 1770 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ38968 fis, clone NT2RI2002316.
ACCESSION AK096287
VERSION AK096287.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1770)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..1770
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2002316"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000021142"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 165..947
/codon_start=1
/protein_id="BAC04747.1"
/translation="MMDPCSVGVQLRTTNECHKTYYTRHTGFKTLQELSSNDMLLLQL
RTGMTLSGNNTICFHHVKIYIDRFEDLQKSCCDPFNIHKKLAKKNLHVIDLDDATFLS
AKFGRQLVPGWKLCPKCTQIINGSVDVDTEDRQKRKPESDGRTAKALRSLQFTNPGRQ
TEFAPETGKREKRRLTKNATAGSDRQVIPAKSKVYDSQGLLIFSGMDLCDCLDEDCLG
CFYACPACGSTKCGAECRCDRKWLYEQIEIEGGEIIHNKHAG"
BASE COUNT 569 a 315 c 380 g 506 t
ORIGIN
1 tcagtggtag cgcggggact ggctgggaag cggtcggtcg agtgtggcct gtgtggactc
61 gcatcttgcc cgaagccggg cggaggagag ctcaagctaa gggtgatcag cccatgacct
121 aaacctccag acaaaataaa acggaaaatt tgctagaatc aagaatgatg gatccatgtt
181 cagttggagt ccagcttcgt actacaaatg agtgccataa aacctactat actcgtcaca
241 caggttttaa gactttgcaa gaattgtcat caaatgatat gcttttactt caacttagaa
301 ctggaatgac actttctggg aacaatacaa tttgctttca tcatgtaaaa atttacattg
361 acagatttga ggatttacag aagtcatgtt gtgacccatt taacatacac aagaaattag
421 ccaaaaaaaa tttgcatgta attgacttag atgatgccac ttttctgagt gctaaatttg
481 gaagacagct tgtacctggt tggaagcttt gtccaaaatg cacacagata atcaatggaa
541 gtgtggatgt tgatactgaa gaccgccaga aaaggaaacc tgagtcagat ggaagaactg
601 ctaaagcttt gaggtcatta caatttacga atccaggaag gcaaactgaa tttgctccag
661 aaactggtaa aagagaaaaa agaaggctta caaaaaatgc aaccgctggt tcagacagac
721 aagtgatacc agcaaagagt aaggtctatg atagccaggg tctcctgatt tttagtggga
781 tggacctctg tgactgcctg gatgaagact gcttaggatg tttctatgct tgtcctgcct
841 gtggttctac caagtgtgga gctgaatgcc gctgtgaccg caagtggctg tatgagcaaa
901 ttgaaattga aggaggagaa ataattcata ataaacatgc tggataatct gcggtaccaa
961 actatggagc ctttaaaggt ctttatttct aaaaatctgt tactctaaga tacattttaa
1021 gcttgattat catatgacaa agattttaaa accatctcag tgtgccctaa tttttcatct
1081 tgggtgcttt aagattcact atttgatata aattcagata ggctattttt cagtagtcag
1141 cgttaagcct gtctggatca atataaacaa gtagggtgta ggcagtcctc tatttgcatg
1201 tttcccatgg gcacaaattt cagtgaccta gatttagttt aaataccagt ttccttacca
1261 ggaaggaaag aaaactggta aggaaactgt tgttgttaaa atctaggtta aaattttagt
1321 tagcacattg taactgagta attacatgaa gtacaaacct ctctgctagc tcttcagtct
1381 acaaatcgct atgtaaataa cagatatgct tcatgattgt gaccagtcat gttatttctt
1441 tcaaattctt ccagtggttt gtccctgtgc atctgttaat tcagttcacg tacagcagag
1501 catgtagtta tgctgtctct ctgtcatcta cttgacattc tatagaagtg aacactcgaa
1561 agaactggtc aacaaagatg aaagtgcagc aaagcaatga aaaatgataa cactggaagt
1621 gaaattttaa tcaaacataa atgaatttgt agaagaagtc actgaccatg ggaatgttgt
1681 tcttgctgct gtgtattcat aggagcttag tgaaggcaaa cttaccaaca caaataagca
1741 aagtggttgc aataaagaca gatacgtccc
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