LOCUS AK096275 2079 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ38956 fis, clone NT2RI2000117, highly similar
to Homo sapiens fatty acid desaturase 1 (FADS1), mRNA.
ACCESSION AK096275
VERSION AK096275.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2079)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..2079
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2000117"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000021130"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 485..1567
/codon_start=1
/note="highly similar to Homo sapiens fatty acid
desaturase 1 (FADS1), mRNA"
/protein_id="BAG53244.1"
/translation="MNSLLIGELSPEQPSFEPTKNKELTDEFRELRATVERMGLMKAN
HVFFLLYLLHILLLDGAAWLTLWVFGTSFLPFLLCAVLLSAVQAQAGWLQHDFGHLSV
FSTSKWNHLLHHFVIGHLKGAPASWWNHMHFQHHAKPNCFRKDPDINMHPFFFALGKI
LSVELGKQKKKYMPYNHQHKYFFLIGPPALLPLYFQWYIFYFVIQRKKWVDLAWMITF
YVRFFLTYVPLLGLKAFLGLFFIVRFLESNWFVWVTQMNHIPMHIDHDRNMDWVSTQL
QATCNVHKSAFNDWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKHGIEYQSKP
LLSAFADIIHSLKESGQLWLDAYLHQ"
BASE COUNT 490 a 557 c 548 g 484 t
ORIGIN
1 attcttatcg ccatggacct taccttgcac aacccaaagt agctgccttg gggcaggggg
61 tggccagagt gcttagggaa atgtggagcc ctacccagaa caacggtgga gggaaaggga
121 agaaacgcag aagtgcccca gttcggacgt agggaagtct tcctcttcgt ggtttttgga
181 gaaccctagc taagagagga aagggactta ttgaaagacc cgcaagaagg gacggaagtc
241 tcatagccct gagagacctc tagctacaga aaagttctcc aggtctgcac tcgacccagg
301 aagtccatct ggcttcacct ctcacttcaa cttgggtaca gccttctggc gggcaggagg
361 atggcctttg gtgcgaacac tgccggagtc cagggggctg gctccctcac ctttcatctt
421 ctcccggcac ttgcaggatc cctttgtggc cttccacatc aacaagggcc ttgtgaagaa
481 gtatatgaac tctctcctga ttggagaact gtctccagag cagcccagct ttgagcccac
541 caagaataaa gagctgacag atgagttccg ggagctgcgg gccacagtgg agcggatggg
601 gctcatgaag gccaaccatg tcttcttcct gctgtacctg ctgcacatct tgctgctgga
661 tggtgcagcc tggctcaccc tttgggtctt tgggacgtcc tttttgccct tcctcctctg
721 tgcggtgctg ctcagtgcag ttcaggccca ggctggctgg ctgcagcatg actttgggca
781 cctgtcggtc ttcagcacct caaagtggaa ccatctgcta catcattttg tgattggcca
841 cctgaagggg gcccccgcca gttggtggaa ccacatgcac ttccagcacc atgccaagcc
901 caactgcttc cgcaaagacc cagacatcaa catgcatccc ttcttctttg ccttggggaa
961 gatcctctct gtggagcttg ggaaacagaa gaaaaaatat atgccgtaca accaccagca
1021 caaatacttc ttcctaattg ggcccccagc cttgctgcct ctctacttcc agtggtatat
1081 tttctatttt gttatccagc gaaagaagtg ggtggacttg gcctggatga ttaccttcta
1141 cgtccgcttc ttcctcactt atgtgccact attggggctg aaagccttcc tgggcctttt
1201 cttcatagtc aggttcctgg aaagcaactg gtttgtgtgg gtgacacaga tgaaccatat
1261 tcccatgcac attgatcatg accggaacat ggactgggtt tccacccagc tccaggccac
1321 atgcaatgtc cacaagtctg ccttcaatga ctggttcagt ggacacctca acttccagat
1381 tgagcaccat ctttttccca cgatgcctcg acacaattac cacaaagtgg ctcccctggt
1441 gcagtccttg tgtgccaagc atggcataga gtaccagtcc aagcccctgc tgtcagcctt
1501 cgccgacatc atccactcac taaaggagtc agggcagctc tggctagatg cctatcttca
1561 ccaataacaa cagccaccct gcccagtctg gaagaagagg aggaagactc tggagccaag
1621 gcagagggga gcttgaggga caatgccact atagtttaat actcagaggg ggttgggttt
1681 ggggacataa agcctctgac tcaaactcct cccttttatc ttctagccac agttctaaga
1741 cccaaagtgg ggggtggaca cagaagtccc taggagggaa ggagctgttg gggcaggggt
1801 gtaaattatt tcctttttct agtttggcac atgcaggtag ttggtgaaca gagagaacca
1861 ggagggtaac agaagaggag ggacctactg aacccagagt caggaagaga tttaacacta
1921 aaattccact catgccgggc gtggtggcac gcgcctgtaa tcccagctac ccaggaggct
1981 gaggcaggag aatcgcttga accggggagg tggaggttgc agtgagctga gatcacgcca
2041 ttgtactcca gcctgggcga cagagcaaga ctccatttc
//