LOCUS AK094164 2714 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ36845 fis, clone ASTRO2013050, weakly similar
to UBIQUITIN-ACTIVATING ENZYME E1.
ACCESSION AK094164
VERSION AK094164.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2714)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Tashiro,H., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..2714
/cell_type="normal astrocytes (NHA5732)"
/clone="ASTRO2013050"
/clone_lib="ASTRO2"
/db_xref="H-InvDB:HIT000019019"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal astrocytes"
/organism="Homo sapiens"
CDS 15..2417
/codon_start=1
/protein_id="BAG52833.1"
/translation="MTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKT
VFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEEL
LKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSP
LCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGA
IGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADA
TLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLR
PLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDK
FESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVEL
ARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQN
AAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISS
EDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIE
PADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVV
FTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKM
LYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHD
TD"
BASE COUNT 909 a 500 c 531 g 774 t
ORIGIN
1 gagaaattaa tggaatgaca ggtttaaatg gatctataca acaaataacg gtgatatcgc
61 cattttcttt tagtattggt gacaccacag aactggaacc atatttacat ggaggcatag
121 ctgtccaagt taagactcct aaaacagttt tttttgaatc actggagagg cagttaaaac
181 atccaaagtg ccttattgtg gattttagca accctgaggc acctttagag attcacacag
241 ctatgcttgc cttggaccag tttcaggaga aatacagtcg caagccaaat gttggatgcc
301 aacaagattc agaagaactg ttgaaactag caacatctat aagtgaaacc ttggaagaga
361 agcctgatgt aaatgctgac attgtgcatt ggctctcttg gactgcccaa ggctttttat
421 ctccacttgc tgcagcagta ggaggtgttg ccagccaaga agtattgaaa gctgtaacag
481 gaaaattttc tcctttgtgc cagtggttat atcttgaagc agcagatatt gttgaatcac
541 taggcaaacc tgaatgtgaa gaatttctcc cacgaggaga tagatatgat gccttaagag
601 cttgcattgg agacactttg tgtcagaaac tgcaaaattt aaacatcttc ttagtagggt
661 gtggagccat aggctgtgaa atgttgaaaa attttgcttt acttggtgtt ggcacaagca
721 aagagaaagg aatgattaca gttacagatc ctgacttgat agagaaatcc aacttaaata
781 gacagttcct atttcgtcct catcacatac agaaacctaa aagctacact gctgctgatg
841 ctactctgaa aataaattct caaataaaga tagatgcaca cctgaacaaa gtatgtccaa
901 ccactgagac catttacaat gatgagttct atactaaaca agatgtaatt attacagcat
961 tagataatgt ggaagccagg agatacgtag acagtcgttg cttagcaaat ctaaggcctc
1021 ttttagattc tggaacaatg ggcactaagg gacacactga agttattgta ccgcatttga
1081 ctgagtctta caatagtcat cgggatcccc cagaagagga aataccattt tgtactctaa
1141 aatcctttcc agctgctatt gaacatacca tacagtgggc aagagataag tttgaaagtt
1201 ccttttccca caaaccttca ttgtttaaca aattttggca aacctattca tctgcagaag
1261 aagtcttaca gaagatacag agtggacaca gtttagaagg ctgttttcaa gtcataaagt
1321 tacttagcag aagacctaga aattggtccc agtgtgtaga attagcaaga ttaaagtttg
1381 aaaaatattt taaccataag gctcttcagc ttcttcactg tttccctctg gacatacgat
1441 taaaagatgg cagtttattt tggcagtcac caaagaggcc accctctcca ataaaatttg
1501 atttaaatga gcctttgcac ctcagtttcc ttcagaatgc tgcaaaacta tatgctacag
1561 tatattgtat tccatttgca gaagaggact tatcagcaga tgccctcttg aatattcttt
1621 cagaagtaaa gattcaggaa ttcaagcctt ccaataaggt tgttcaaaca gatgaaactg
1681 caaggaaacc agaccatgtt cctattagca gtgaagatga gaggaatgca attttccaac
1741 tagaaaaggc tattttatct aatgaagcca ccaaaagtga ccttcagatg gcagtgcttt
1801 catttgaaaa agatgatgat cataatggac acatagattt catcacagct gcatcaaatc
1861 ttcgtgccaa aatgtacagc attgaaccag ctgaccgttt caaaacaaag cgcatagctg
1921 gtaaaattat acctgctata gcaacaacca ctgctacagt ttctggcttg gttgccttgg
1981 agatgatcaa agtaactggt ggctatccat ttgaagctta caaaaattgt tttcttaact
2041 tagccattcc aattgtagta tttacagaga caactgaagt aaggaaaact aaaatcagaa
2101 atggaatatc atttacaatt tgggatcgat ggaccgtaca tggaaaagaa gatttcaccc
2161 tcttggattt cataaatgca gtcaaagaga agtatggaat tgagccaaca atggtggtac
2221 agggagtcaa aatgctttat gttcctgtaa tgcctggtca tgcaaaaaga ttgaagttaa
2281 caatgcataa acttgtaaaa cctactactg aaaagaaata tgtggatctt actgtgtcat
2341 ttgctccaga cattgatgga gatgaagatt tgccgggacc tccagtaaga tactacttca
2401 gtcatgacac tgattaatac aagttgtctt aacgttactc caggaccact tgattttgga
2461 aagagtgcac ttaattcaga agctaaagaa aatcagttca taatactatg gatttctctt
2521 tcattaagcc ttaattttaa gggaaacatc agtaagaaac tgcactgaag aattataaaa
2581 cattttgggg catagcatac acttgtctaa cggttcacac gtggctatga tcacaagcaa
2641 ctttgaactg gaatgctatt tacaaaagtt ttgtgtatta atctgtgtat taatctctct
2701 ggataaaaaa aagg
//