LOCUS AK092244 3255 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ34925 fis, clone NT2RP7003148, highly similar
to Meckelin.
ACCESSION AK092244
VERSION AK092244.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3255)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Tashiro,H., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..3255
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7003148"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000017111"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 203..2947
/codon_start=1
/note="highly similar to Meckelin"
/protein_id="BAG52507.1"
/translation="MSLSHWPYFRLVLNFRPRVICLPRPPKVLGYRLEPPHLTLACTL
EGMYNIIEEILERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTFVNTSRS
CACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYA
NLTSCQALGSMCVMNMNSYDFATFDACGLFQFIFENTAGLSTVHSISFWRQNLPWLFY
GDQLGLAPQVLSSTSLPTNFSFKGEDQNTKLKFVAASYDIRGNFLKWQTLEGGVLQLC
PDTETRLNAAYSFGTTYQQNCEIPISKILIDFPTPIFYDVYLEYTDENQHQYILAVPV
LNLNLQHNKIFVNQDSNSGKWLLTRRIFLVDAVSGRENDLGTQPRVIRVATQISLSVH
LVPNTINGNIYPPLITIAYSDIDIKDANSQSVKVSFSVTYEMDHGEAHVQTDIALGVL
GGLAVLASLLKTAGWKRRIGSPMIDLQTVVKFLVYYAGDLANVFFIITVGTGLYWLIF
FKAQKSVSVLLPMPVQEERFVTYVGCAFALKALQFLHKLISQITIDVFFIDWERPKGK
VLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINSLFQVLTVLFFLEVVGFK
NLALMDSSSSLSRNPPSYIAPYSCILRYAVSAALWLAIGIIQVVFFAVFYERFIEDKI
RQFVDLCSMSNISVFLLSHKCFGYYIHGRSVHGHADTNMEEMNMNLKREAENLCSQRG
LVPNTDGQTFEIAISNQMRQHYDRIHETLIRKNGPARLLSSSASTFEQSIKAYHMMNK
FLGSFIDHVHKEMDYFIKDKLLLERILGMEFMEPMEKSIFYNDEGYSFSSVLYYGNEA
TLLIFDLLFFCVVDLACQNFILASFLTYLQQEIFRYIRNTVGQKNLASKTLVDQRFLI
"
BASE COUNT 959 a 588 c 663 g 1045 t
ORIGIN
1 aggctgtgag gcttccagcg tcggcccaga ccttctcttt ccctttccag cagccggaga
61 agtgcgacaa caaccagtac tttgatatct ccgccctctc gtgtgttcct tgtggagcta
121 accagaggca agatgcccga ggtgttacag aagatggctg gaactgcatt tcttgcccta
181 gtgacttaac tgccgaagga aaatgtcact gtcccattgg ccatatttta ggctggtctt
241 gaacttccga cctcgggtga tctgcctgcc tcggcctccc aaagtgctgg gatacaggct
301 tgagccaccg cacctgacct tggcatgtac tttagaagga atgtacaaca taattgaaga
361 aattttggaa agagacatta atggaacatt gttgtctcaa gcaacttgtg agctctgtga
421 tggaaatgaa aactctttta tggtagtaaa tgctttagga gacaggtgcg tccgatgtga
481 gccaacattt gttaatacca gcaggtcctg tgcatgttca gaacctaaca ttttaacagg
541 gggattatgt ttcagcagca cagggaattt tcctctacgt agaatttcag ctgcacgtta
601 tggagaagtt ggcatgtctt taacttcaga atggtttgca aagtatttgc aatcatcagc
661 agctgcatgt tgggtatatg ccaatctaac atcttgtcaa gctcttggaa gtatgtgtgt
721 gatgaacatg aattcttacg actttgccac atttgatgca tgtggactat ttcagtttat
781 ctttgaaaat actgctggac tgagcactgt tcattctatt tcattttgga gacagaatct
841 tccttggctg ttttatggag accagttagg attagcacct caagtgctca gttctacctc
901 tcttcctaca aatttcagtt ttaaaggaga agaccagaat acaaaactga agtttgttgc
961 tgcttcctat gatataagag gaaattttct caagtggcaa actttagaag gaggtgtttt
1021 acagctttgt ccagacacag agacaaggct aaatgctgct tattcatttg gaacaaccta
1081 ccaacaaaat tgtgagattc ctatctctaa gatcttaatt gactttccca ctcctatatt
1141 ttatgatgtg taccttgaat atactgatga aaatcaacat caatatattt tggctgtgcc
1201 tgtgttaaac ctaaatcttc aacataataa gatatttgtg aaccaagaca gcaactctgg
1261 aaagtggctt ctaactcggc gcattttctt agtggatgca gtaagtggac gagaaaatga
1321 cttaggaact cagccaagag taattcgagt tgctactcaa atatcactga gtgtccacct
1381 tgtacccaac acaataaatg gaaacatcta ccctccctta atcaccattg cctacagtga
1441 cattgatatc aaagatgcca acagccagtc tgtgaaggtt tccttctcag tcacatatga
1501 aatggatcat ggagaagcac atgtccagac agatattgct ttgggtgtat tgggtgggct
1561 agctgtttta gcatctcttt tgaagacagc aggatggaag aggcgcattg ggagtcccat
1621 gattgattta cagacagttg tgaaattctt ggtgtactat gctggtgatc tggccaatgt
1681 tttctttatc atcacagtgg gaacaggtct ttactggctt attttcttca aagcacagaa
1741 gtctgtgtct gttttgctgc caatgccagt tcaggaagaa cgttttgtca cttatgttgg
1801 atgtgccttt gctctgaagg cactacaatt tttgcataag ctcatatccc agattacaat
1861 agatgtattc tttattgatt gggagcgacc taaaggaaag gttcttaaag ctgttgaagg
1921 tgagggtggt gtacgaagtg ccactgttcc tgtaagcata tggagaacat attttgtagc
1981 aaatgaatgg aatgaaattc agactgtgag aaaaattaat tcactctttc aagtacttac
2041 tgtcctcttc tttttggagg ttgtgggatt caagaactta gcattaatgg actcatcttc
2101 tagtctttct agaaacccac ctagctacat agctccttat agctgcattt tgagatatgc
2161 agtgtctgct gctctttggc tagccattgg aattatacag gtcgtgttct ttgctgtctt
2221 ttatgagaga tttatagaag ataaaattcg acagttcgtt gatttatgct ctatgagtaa
2281 tatatcagtg tttctgttat cccacaaatg ttttggatat tacattcatg gtagatcagt
2341 acatgggcat gcagatacta atatggaaga aatgaatatg aaccttaaaa gagaagcgga
2401 aaatttgtgt agccagagag gtttggtacc caacacagat ggtcagactt ttgagattgc
2461 aatttctaac cagatgagac aacattatga cagaattcat gagacactaa taaggaaaaa
2521 tggtcctgct agactactga gttcatcagc aagtactttt gagcagagta taaaagcata
2581 tcatatgatg aataaatttc ttggctcctt cattgaccat gttcataagg aaatggatta
2641 ctttataaaa gataagttgc ttcttgaaag aattcttgga atggaattca tggaaccaat
2701 ggaaaaaagc atcttttaca atgatgaagg ttattctttc agcagtgtcc tgtattatgg
2761 aaatgaagct actcttctta tttttgatct gctgttcttc tgtgttgtgg atttggcttg
2821 ccaaaatttt attttagcat ccttccttac atatctacaa caagagattt ttagatatat
2881 ccgtaataca gtaggacaaa agaatttggc atccaaaaca ttggtggatc aaagattttt
2941 gatttaactt cctgaataaa taacttaaag actcagtata atcatggcca aaaaaaagtc
3001 atgatatcag gttagtttgc catatttttt aaaacttata atgcagacta ttctgttttt
3061 gttttttatt tgcagaaaga tttattttta taacttggga atatataacc tgatataata
3121 gaaaatactg ttttcccatt gtgtgtagta tttaatgcag taaataataa attaatactg
3181 acagtgaggc caaaaaaaat ctaaatgttg ctttcagcat aatacttttt aatgctaatt
3241 aaaacagtcc ccatc
//