LOCUS AK092020 3476 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ34701 fis, clone MESAN2002724, weakly similar
to Rho guanine nucleotide exchange factor (GEF) 10.
ACCESSION AK092020
VERSION AK092020.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3476)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Tashiro,H., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..3476
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2002724"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000016887"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 39..1940
/codon_start=1
/protein_id="BAC03792.1"
/translation="MLPSSSWGKRKLRGRLGWVRSDARGVMWARQEGLHHPHPHAVIR
CPSSSSSSVSCSGDSEEEDMGLLEVSVSDIKPPAPELGPMPEGLSPQQVVRRHILGSI
VQSEGSYVESLKRILQDYRNPLMEMEPKALSARKCQVVFFRVKEILHCHSMFQIALSS
RVAEWDSTEKIGDLFVASDMLKNTPRGHPDRLSLQLALTELETLAEKLNEQKRLADQV
AEIQQLTKSVSDRSSLNKLLTSGQRQLLLCETLTETVYGDRGQLIKSKERRVFLLNDM
LVCANINFKGQLEISSLVPLGPKYVVKWNTALPQVQVVEVGQDGGTYDKDNVLIQHSG
AKKASASGQAQNKVYLGPPRLFQELQDLQKDLAVVEQITLLISTLHGTYQNLNMTVAQ
DWCLALQRLMRVKEEEIHSANKCRLRLLLPGKPDKSGRPISFMVVFITPNPLSKISWV
NRLHLAKIGLREENQPGWLCPDEDKKSKAPFWCPILACCIPAFSSRALSLQLGALVHS
PVNCPLLGFSAVSTSLPQGYLWVGGGQEGAGGQVEIFSLNRPSPRTVKSFPLAAPVLC
MEYIPELEEEAESRDESPTVADPSATVHPTICLGLQDGSILLYSSVDTGTQCLVSCRS
PGLQPVLCL"
BASE COUNT 686 a 1072 c 1077 g 641 t
ORIGIN
1 attaatagcc gtggcagggc tcgaatcaca ggccaagaat gttacccagc agctcgtggg
61 ggaagaggaa gctgcgaggc aggctggggt gggtgcggag tgatgcccgg ggcgtgatgt
121 gggcccggca ggagggtctg caccaccccc acccccacgc cgtcatccgc tgtccctcct
181 cctcctcctc ctctgtctcc tgctcaggtg actcggagga ggaggacatg gggctcctgg
241 aggtcagcgt ttcggacatc aagcccccag ccccagagct gggccccatg ccagagggcc
301 tgagccctca gcaggtggtc cggaggcata tcctgggctc catcgtgcag agcgaaggca
361 gctacgtgga gtctctgaag cggatactcc aggactaccg caaccccctg atggagatgg
421 agcccaaggc gctgagcgcc cgcaagtgcc aggtggtgtt cttccgcgtg aaggagatcc
481 tgcactgcca ctccatgttc cagatcgccc tgtcctcccg cgtggctgag tgggattcca
541 ccgagaagat cggggacctc ttcgtggcct cggacatgct gaagaacacc cccaggggcc
601 atccggacag gctgtcgctg cagctggccc tcacagagct ggagacgctg gctgagaagc
661 tgaacgagca gaagcggctg gctgaccagg tggctgagat ccagcagctg accaagagcg
721 tcagtgaccg cagcagcctc aacaagctgt tgacctcagg ccagcggcag ctgctcctgt
781 gtgagacgtt gacggagacc gtgtacggtg accgcgggca gctaattaag tccaaggagc
841 gtcgggtctt cctgctcaac gacatgcttg tctgtgccaa catcaacttc aagggccagc
901 tggagatcag cagcctggtg cccctggggc ccaagtatgt ggtgaagtgg aacacggcgc
961 tgccccaggt gcaggtggtg gaggtgggcc aggacggtgg cacctatgac aaggacaatg
1021 tgctcatcca gcactcaggc gccaagaagg cctctgcctc agggcaggct cagaataagg
1081 tgtacctcgg ccccccacgc ctcttccagg agctgcagga cctgcagaag gacctggccg
1141 tggtggagca gatcacgctt ctcatcagca cgctgcacgg cacctaccag aacctgaaca
1201 tgactgtggc tcaagactgg tgcctggccc tgcagaggct gatgcgggtg aaggaggaag
1261 agatccactc ggccaacaag tgccgtctca ggctcctgct tcctgggaaa cccgacaagt
1321 ccggccgccc cattagcttc atggtggttt tcatcacccc caaccccctg agcaagattt
1381 cctgggtcaa caggttacat ttggccaaaa tcggactccg ggaggagaac cagccaggct
1441 ggctatgccc ggatgaggac aagaagagca aagccccatt ctggtgcccg atcctggcct
1501 gctgcatccc tgccttctcc tcccgggcac tcagcctgca gcttggggcc ctggtccaca
1561 gtcctgtcaa ctgtcccctg ctgggtttct cagcagtcag cacctccctt ccacagggct
1621 acctctgggt cgggggcgga caggaaggcg cagggggcca ggtggaaatc ttttccttga
1681 accggccctc gccccgcacc gtcaagtcct tcccactggc agcccctgtg ctctgcatgg
1741 agtatatccc ggagctggag gaggaggcgg agagcagaga cgagagcccg acagttgctg
1801 acccctcggc cacggtgcat ccaaccatct gcctcgggct ccaggatggc agcatcctcc
1861 tctacagcag tgtggacact ggcacccagt gcctggtgag ctgcaggagc ccaggtctgc
1921 agcctgtgct ctgcctgtga cacagcccct tccacctgct cgctggcctg caggatggga
1981 cccttgctgc ttaccctcgg accagcggag gtgtcctgtg ggacctggag agccctcccg
2041 tgtgcctgac tgtggggccc gggcctgtcc gcaccctgtt gagcctggag gatgccgtgt
2101 gggccagctg tgggccccgg gtcactgtcc tggaagccac caccctgcag cctcagcaaa
2161 gcttcgaggc gcaccaggac gaggcagtga gcgtgacaca catggtgaag gcgggcagcg
2221 gcgtctggat ggccttctcc tccggcacct ccatccgcct cttccacact gagaccctgg
2281 agcatctgca agagatcaac atcgccacca ggaccacctt cctcctgcca ggccagaagc
2341 acttgtgtgt caccagcctc ctgatctgcc agggtctgct ctgggtgggc actgaccagg
2401 gtgtcatcgt cctgctgccc gtgcctcggc tggaaggcat ccccaagatc acagggaaag
2461 gcatggtctc actcaacggg cactgtgggc ctgtggcctt cctggctgtg gctaccagca
2521 tcctggcccc tgacatcctg cggagtgacc aggaggaggc tgaggggccc cgggctgagg
2581 aggacaagcc agacgggcag gcacacgagc ccatgcccga tagccacgtg ggccgagagc
2641 tgacccgcaa gaagggcatc ctcttgcagt accgcctgcg ctccaccgca cacctcccgg
2701 gcccgctgct ctccatgcgg gagccggcgc ctgctgatgg cgcagctttg gagcacagcg
2761 aggaggacgg ctccatttac gagatggccg acgaccccga catctgggtg cgcagccggc
2821 cctgcgcccg cgacgcccac cgcaaggaga tttgctctgt ggccatcatc tccggcgggc
2881 agggctaccg caactttggc agcgctctgg gcagcagtgg gaggcaggcc ccgtgtgggg
2941 agacggacag caccctcctc atctggcagg tgcccttgat gctatagcgc ctcccctctc
3001 ccctcagagg gcacagctgc aggcctgacc aaggccacgc ccggctctcg tgctctagga
3061 cctgcacggg acttgtggat gggcctggac tctccagaaa ctacttgggc agagcaaagg
3121 aaaacctctt gttttaaaaa aatttttttc agagtgtttt ggggaggagt tttagggctt
3181 ggggagaggg aggacacatc tggaggaaat ggccttcttt ttaaaagcaa aaaaacacaa
3241 aacctcacaa ctgcctggca agcccagtat cacttgtttg ggccctagcg ggactccaag
3301 gcagccacac gcccctcctg gaagggtgtg tgcgtgtgag tgtgtgcgag tgtgtgggct
3361 ggtgtgtgaa tatctataaa taagtatata tggtgtatat tatatgtgta taaataaaat
3421 ctgtacatat tggagctctg ggagatgctg gaataaaaga caagagttac atctgg
//