LOCUS AK087557 2389 bp mRNA linear HTC 06-OCT-2010
DEFINITION Mus musculus 0 day neonate eyeball cDNA, RIKEN full-length
enriched library, clone:E130320B06 product:thymidine kinase 2,
mitochondrial, full insert sequence.
ACCESSION AK087557
VERSION AK087557.1
KEYWORDS HTC_FLI; HTC; CAP trapper.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 2389)
AUTHORS Adachi,J., Aizawa,K., Akimura,T., Arakawa,T., Bono,H.,
Carninci,P., Fukuda,S., Furuno,M., Hanagaki,T., Hara,A.,
Hashizume,W., Hayashida,K., Hayatsu,N., Hiramoto,K., Hiraoka,T.,
Hirozane,T., Hori,F., Imotani,K., Ishii,Y., Itoh,M., Kagawa,I.,
Kasukawa,T., Katoh,H., Kawai,J., Kojima,Y., Kondo,S., Konno,H.,
Kouda,M., Koya,S., Kurihara,C., Matsuyama,T., Miyazaki,A.,
Murata,M., Nakamura,M., Nishi,K., Nomura,K., Numazaki,R., Ohno,M.,
Ohsato,N., Okazaki,Y., Saito,R., Saitoh,H., Sakai,C., Sakai,K.,
Sakazume,N., Sano,H., Sasaki,D., Shibata,K., Shinagawa,A.,
Shiraki,T., Sogabe,Y., Tagami,M., Tagawa,A., Takahashi,F.,
Takaku-Akahira,S., Takeda,Y., Tanaka,T., Tomaru,A., Toya,T.,
Yasunishi,A., Muramatsu,M. and Hayashizaki,Y.
TITLE Direct Submission
JOURNAL Submitted (16-APR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Yoshihide Hayashizaki
The Institute of Physical and Chemical Research (RIKEN), Omics
Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
URL :http://www.osc.riken.jp/
REFERENCE 2
AUTHORS
CONSRTM The FANTOM Consortium, Riken Genome Exploration Research Group and
Genome Science Group (Genome Network Project Core Group)
TITLE The Transcriptional Landscape of the Mammalian Genome
JOURNAL Science 309, 1559-1563 (2005)
REFERENCE 3
AUTHORS
CONSRTM RIKEN Genome Exploration Research Group and Genome Science Group
(Genome Network Project Core Group) and the FANTOM Consortium
TITLE Antisense Transcription in the Mammalian Transcriptome
JOURNAL Science 309, 1564-1566 (2005)
REFERENCE 4
AUTHORS
CONSRTM The FANTOM Consortium and the RIKEN Genome Exploration Research
Group Phase I and II Team
TITLE Analysis of the mouse transcriptome based on functional annotation
of 60,770 full-length cDNAs
JOURNAL Nature 420, 563-573 (2002)
REFERENCE 5
AUTHORS
CONSRTM The RIKEN Genome Exploration Research Group Phase II Team and the
FANTOM Consortium
TITLE Functional annotation of a full-length mouse cDNA collection
JOURNAL Nature 409, 685-690 (2001)
REFERENCE 6
AUTHORS Carninci,P. and Hayashizaki,Y.
TITLE High-efficiency full-length cDNA cloning
JOURNAL Meth. Enzymol. 303, 19-44 (1999)
REFERENCE 7
AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K.,
Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
TITLE Normalization and subtraction of cap-trapper-selected cDNAs to
prepare full-length cDNA libraries for rapid discovery of new genes
JOURNAL Genome Res. 10, 1617-1630 (2000)
REFERENCE 8
AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N.,
Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai,T.,
Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M.,
Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S.,
Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y.,
Izawa,M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K.,
Tanaka,T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M.,
Inoue,Y., Kira,A. and Hayashizaki,Y.
TITLE RIKEN integrated sequence analysis (RISA) system-384-format
sequencing pipeline with 384 multicapillary sequencer
JOURNAL Genome Res. 10, 1757-1771 (2000)
COMMENT cDNA library was prepared and sequenced in Mouse Genome
Encyclopedia Project of Genome Exploration Research Group in Riken
Genomic Sciences Center and Genome Science Laboratory in RIKEN.
Division of Experimental Animal Research in Riken contributed to
prepare mouse tissues.
Please visit our web site for further details.
URL:http://www.osc.riken.jp/
URL:http://fantom.gsc.riken.jp/
clone information is available at:
http://fantom.gsc.riken.jp/3/db/annotate/
main.cgi?masterid=E130320B06
FEATURES Location/Qualifiers
source 1..2389
/clone="E130320B06"
/clone_lib="RIKEN full-length enriched mouse cDNA library"
/db_xref="FANTOM_DB:E130320B06"
/db_xref="MGI:2425816"
/db_xref="taxon:10090"
/dev_stage="0 day neonate"
/mol_type="mRNA"
/organism="Mus musculus"
/strain="C57BL/6J"
/tissue_type="eyeball"
CDS 27..839
/codon_start=1
/note="putative"
/note="thymidine kinase 2, mitochondrial (MGD|MGI:1913266
GB|NM_021028, evidence: BLASTN, 99%, match=2366)"
/protein_id="BAC39926.1"
/transl_table=1
/translation="MLLRSLRSWAARSLRSVGPGSSGSPGSLDSGAGPLWAPRRAWPP
DKDRENDKEKKAVVCIEGNIASGKTTCLEFFSNTTDVEVLMEPVLKWRNVHGHNPLSL
MYHDASRWGLTLQTYVQLTMLDQHTRPQMSPVRLMERSIYSARYIFVENLYRSGKMPE
VDYAILSEWFDWIVRNIDVSVDLIVYLRTTPEICYQRLKMRCREEEKVIPMEYLHAIH
RLYEEWLVNGSLFPAAAPVLVIEADHNLEKMLELFEQNRARILTPENWKHGP"
regulatory 2374..2379
/note="putative"
/regulatory_class="polyA_signal_sequence"
polyA_site 2389
/note="putative"
BASE COUNT 559 a 596 c 653 g 581 t
ORIGIN
1 gaggacttgc acatcagcca gggactatgc tgctgcggtc gctgcgtagt tgggctgccc
61 ggtccctgcg tagcgtgggc ccggggagct ccgggagccc cgggagcctc gattcgggcg
121 ccgggccgct gtgggcacct cgccgcgcct ggcctccgga taaagataga gaaaatgata
181 aggagaaaaa agcagtggtt tgtattgagg gcaatattgc aagtgggaag acgacatgcc
241 tggagttctt ctccaataca acagacgtcg aggtgttaat ggagcctgtg ctcaagtgga
301 gaaatgtcca tggccataac cctctgagcc tcatgtacca tgatgccagc cgatggggcc
361 tcacactgca gacgtacgtg cagctcacca tgctggacca gcacacgcgc cctcagatgt
421 cacctgtacg gttgatggaa aggtcaattt acagcgcaag atacattttt gtagaaaacc
481 tgtatagaag tgggaagatg cccgaggttg actacgcgat tctgtctgag tggtttgact
541 ggatcgtcag gaacattgat gtctctgttg atctgatagt ttatctgcga accactcccg
601 aaatctgcta ccagagatta aagatgaggt gccgggaaga ggagaaagtc attccgatgg
661 aatacctcca tgctattcac cgcctctacg aggagtggct ggtcaacggg agcctcttcc
721 cagctgcagc ccctgttctg gtgattgagg ctgaccacaa cttggagaaa atgttagaac
781 tctttgaaca aaaccgggcc cggatattaa ctccagagaa ctggaagcat ggaccctagg
841 actgagtgat ctacaagaca gcgccaggaa cagcccagcc agggccaggt tagctgctag
901 gagcatttgg aagaatctac tcttaggagg gtctgttatc taatcagatc tattttctca
961 atggtctttt cactgcagta gcaaatggct tttgtctgtc agatgcttgc agcatacatc
1021 cctcctggtg cccctcctgg tggcttccta acgatgaagc cctgtgttaa aatcactgac
1081 tgtatccctg gggttgttct ggatcctctg catcaggtca cctcatgggt gagggagcag
1141 tcttccaccc caggcaagga tttgaagccc agagaggtgt ggggactcgt caagccagag
1201 cattgtggag ggagctggga ccattcagat acctggtttg ggctgcctcc tcctaagctg
1261 ttccccaccg ccctgctgct ctgagcacca gcccccgcct ctgtgagaag agtggacagt
1321 ttgtgaattc catcatcttt cagtaccggg ccagttcaag gagaccagac atcactaggt
1381 gttggcacac ctctcttatt tggcatgagc ccagggtgtc ctggtatgag ggtactgtga
1441 cctctatcca ttcctttgtc acctggacct cggtgtccac tggggactcc tggatcggcc
1501 tctgcttggt gccagagtcc ttgaggatga gtcctcccca gactctacca gttccctgtc
1561 gttttgtgtc agaaaaaagg gggttatggg acctggacac tttacccttt gctgagcgag
1621 gccaagggca gccagctgag cttccaagac cccatcactc tctcctgtcc ttgtgtgagg
1681 agcagtgtcc ccttgctcca gggagagtga cagtggcctt aactcctttg aagtgttttt
1741 ccatctgagc agatgtcatg gaccacgagc atgaagaagt catctctatt ggatcagaaa
1801 ataccttagc tttgctgagc cgtaactgtg cactgaaggt gcagctgtta cttgaatcag
1861 tcatgaccaa gagaggccgg ccattcctca ggaccgtgag actggaaagt catggtttgc
1921 caacaaagac aatttaagcc ttgctgctct tgacattcta gaatggatgg agtgcatctg
1981 gaaaagactg gacacagatg atacagggga atgatgaccg tataccctgg agaagcacag
2041 cacaggctgg ggagggggct gcaggggagc cctgctcccc tgtttagctg cctgggagct
2101 agaaagaggg ccaagagtcc agctcttggt cctttctgtt tggaccaatt cttggccctt
2161 ttggtagtat gaagagtagc atccagggag attgtccatc agcacctgca gtccagcaat
2221 ggataaacct gagaaaggca ggaactttga accagcagta ctcatggggg catctttaga
2281 atttgcaaac gaacagtcta gtacttgccc tttgctctac cagaacactt gttttgggga
2341 gaaatgtgct gtttctcttt ctctctagta gccaataaaa ttctacagc
//