LOCUS AK075551 2372 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA PSEC0251 fis, clone NT2RP3003097.
ACCESSION AK075551
VERSION AK075551.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2372)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nishikawa,T., Suzuki,Y., Kawai-Hio,Y., Hayashi,K.,
Ishii,S., Saito,K., Yamamoto,J., Wakamatsu,A., Nagai,T.,
Nakamura,Y., Nagahari,K., Sugano,S. and Isogai,T.
TITLE HRI human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT HRI human cDNA sequencing project; cDNA 5'- & 3'-end one pass
sequencing, clone selection and full insert sequencing: Helix
Research Institute (supported by Japan Key Technology Center
etc.); cDNA library construction: Institute of Medical Science,
University of Tokyo, Laboratory of Genome Structure, Human Genome
Center.
FEATURES Location/Qualifiers
source 1..2372
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP3003097"
/clone_lib="NT2RP3"
/db_xref="H-InvDB:HIT000082584"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 77..1258
/codon_start=1
/protein_id="BAC11694.1"
/translation="MGLLRGGLPCARAMARLGAVRSHYCALLLAAALAVCAFYYLGSG
RETFSSATKRLKEARAGAPAAPSPPALELARGSVAPAPGAKAKSLEGGGAGPVDYHLL
MMFTKAEHNAALQAKARVALRSLLRLAKFEAHEVLNLHFVSEEASREVAKGLLRELLP
PAAGFKCKVIFHDVAVLTDKLFPIVEAMQKHFSAGLGTYYSDSIFFLSVAMHQIMPKE
ILQIIQLDLDLKFKTNIRELFEEFDSFLPGAIIGIAREMQPVYRHTFWQFRHENPQTR
VGGPPPEGLPGFNSGVMLLNLEAMRQSPLYSRLLEPAQVQQLADKYHFRGHLGDQDFF
TMIGMEHPKLFHVLDCTWNRQLCTWWRDHGYSDVFEAYFRCEGHVKIYHGNCNTPIPE
D"
BASE COUNT 491 a 727 c 697 g 457 t
ORIGIN
1 aagtggccgc cggcgtccgg gtacccgagg gctctcccgc gttgctggca ccgctggcgc
61 cgcggtctcg tagcgcatgg gcctcctccg aggcgggctc ccatgcgctc gggccatggc
121 gcgcctgggc gctgtgcgct cccactactg cgccctgctg ctggccgcgg cgctggccgt
181 ctgcgccttc tactacctcg gctcaggccg ggagaccttc tccagcgcca ccaagaggct
241 gaaggaggcc cgcgccgggg ctcccgccgc gccctcgccg cccgcgctgg agctagcgcg
301 gggctccgtg gcgccagccc ccggcgcgaa ggccaagagc ttggagggcg gcggtgccgg
361 gccggtggac taccacctgc tgatgatgtt caccaaggcg gagcacaatg ccgcgctgca
421 ggccaaggcc cgcgtcgcgc tgcgctcact gctgcgcctc gccaagttcg aggcgcacga
481 ggtgcttaac cttcacttcg tgagcgagga ggccagccgc gaggtggcca agggcctgct
541 gcgggagctc ctgccgcccg ccgctggctt caagtgcaag gtcatcttcc acgatgttgc
601 tgtgctgacg gataagctct tccccatcgt ggaggccatg cagaagcact tcagtgctgg
661 cttgggaacc tactacagtg actccatctt cttcctctcg gtcgccatgc atcagatcat
721 gcccaaagag atcctgcaga tcattcagct ggacctagac ctgaagttta agaccaacat
781 ccgggagttg tttgaggaat ttgacagttt cctgccaggc gccatcatcg gcatagcccg
841 ggagatgcag ccagtttaca ggcacacatt ctggcagttc cgccatgaga acccccagac
901 ccgggttggg ggcccgcccc ccgaggggct gccgggcttc aacagcgggg tgatgttgct
961 gaacctggag gccatgcgcc agtccccgct ctacagccgc ctgctggagc cggcgcaggt
1021 gcagcagctg gccgacaagt accacttccg cggccacctc ggggaccagg acttcttcac
1081 catgatcggc atggagcacc ccaagctctt ccatgtgctg gactgtacct ggaaccggca
1141 gctgtgcacc tggtggaggg accatggcta cagtgacgtc ttcgaggcct atttcaggtg
1201 tgagggccac gtcaagatct accacgggaa ctgcaacact cccatcccgg aggactaggc
1261 gctccccgtg ccttgccccc ggggcctcca gatctggggg gaggacaggg ttccttggga
1321 cagacccaag ggcagtgtct gacccgctag agagacaccg cagggaagtc ctgtgattaa
1381 ggtgctgaga cctcctgccg ggcaggtcac tgtgctgagg ccacccgcca aggactggcc
1441 tgtgcactgc tggtgcttgg gaccttattc ctccagggag caggcgacac aaaggacacg
1501 catgtccagt gggctgggaa gcaagcactt gaagagaagg aaggggagaa agggtccccc
1561 ttgctgtctg cctctgagga atggaaatcc tttagacccg gccttttttg gaccaatata
1621 aatttaattt aaattgacag ccttccattt ttcgagaaag tacaaacaga actgctttag
1681 cacccatcga gccccaaacg ggtaaggtaa gccaaggttt taatgaccag cccagtatct
1741 aagcttccaa acgaatacca gcccatcaca tactcaccct gggaggctgc tgcacgcgca
1801 ttctcctgat gctcacgtca cttggtgtag gtttcaggat cgcctctttg aggaaggact
1861 tcaggaccaa ctggggcctg cataagaaaa cttatctcat tattagagta ctcacagctt
1921 gtatctccca gctacatcct agaaccccat tgtcctttat tccaccaaac cagctccagg
1981 tgaccagact ctactcagaa agcaaattcg tcatcaaaga acagagactg gccaccacaa
2041 ggacatgcag gagaactgtc gggaccagga agactcattc caaaaagccc aggccgggca
2101 cagtcgtcaa gcctgtaatc ccaacacttt gggagaccga ggtgggggta tcgattgagc
2161 ctcggaggtc gagatcagcc tgggaaacac agggaggccc ccatcgctac aaaatatttt
2221 aaaaattagc caggtgtggt ggcttgtgct tgttgtcccg gctacttggg aggctgaagt
2281 gggagggtgg cttgagtcca ggagttcact gcactgagct gtgatcacac cactgcactc
2341 cagcctggac gacagagtga gacgtccatc tc
//