LOCUS AK075543 2835 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA PSEC0243 fis, clone NT2RP3000326.
ACCESSION AK075543
VERSION AK075543.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2835)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nishikawa,T., Suzuki,Y., Kawai-Hio,Y., Hayashi,K.,
Ishii,S., Saito,K., Yamamoto,J., Wakamatsu,A., Nagai,T.,
Nakamura,Y., Nagahari,K., Sugano,S. and Isogai,T.
TITLE HRI human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT HRI human cDNA sequencing project; cDNA 5'- & 3'-end one pass
sequencing, clone selection and full insert sequencing: Helix
Research Institute (supported by Japan Key Technology Center
etc.); cDNA library construction: Institute of Medical Science,
University of Tokyo, Laboratory of Genome Structure, Human Genome
Center.
FEATURES Location/Qualifiers
source 1..2835
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP3000326"
/clone_lib="NT2RP3"
/db_xref="H-InvDB:HIT000082576"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 160..2391
/codon_start=1
/protein_id="BAC11686.1"
/translation="MAQASRSGSLPPLVIVPPLRAQPGGTGEEQWERSRTGGLRWEVH
CWPSGTSGGTPWWPTPADVSEDYEADAAAWRRGPAGGGPIPPALQRLRAVLLRLHRER
EQLLQARDCAYHLQSAVRLMKTLSPGSPSGGPSPLPQWYRDLQLHPSQGAVLRIGPGE
TLEPLLLARPIGLAAQCLEAVIEMQLRALGREPASPGLSSQLAELLFALPAYHTLQRK
ALSHVPGAARPFPTSRVLRLLTGERGCQVASRLDEALQGSALRDQLRRRCQEEGDLLP
GLLGLVGGVAGSASCGLGLGGAGALWCQYWTLLWAACAQSLDLNLGPWRDPRATAQQL
SQALGQASLPQECEKELASLCHRLLHQSLIWSWDQGFCQALGSALGGQSSLPTSSGTA
ELLQQLFPPLLDALREPRLRRIFCQPAGLCTLQTTLLWFLGRAQQYLAAWDPASFLLL
IQKDLPPLLHEAEALYSLASEESLALEVEQQLGLEIQKLTAQIQLLPEESLSVFSQEC
HKQAMQGFKLYMPRGRYWRLRLCPEPPSAPSEYAGLVVRTVLEPVLQGLQGLPPQAQA
PALGQALTAIVGAWLDHILTHGIRFSLQGALQLKQDFGVVRELLEEEQWSLSPDLRQT
LLMLSIFQQLDGALLCLLQQPLPKSQVHRRPPCCCACQEVQTTKLPSSCLNSLESLEP
PLQPGTSPAQTGQLQSTLGGRGPSPEGYLVGNQQAWLALRQHQRPRWHLPFFSCLGTS
PES"
BASE COUNT 544 a 844 c 892 g 555 t
ORIGIN
1 aagcctccca ttacggagca cgagagagtc catgaaggtc cccgcgactc ccggactgga
61 gaaaacggct cttgcgatgg ggcgaagtcc gagctgcggc gggcgttggt ccgtgcaggg
121 aagtgggaat cgttaggttc gttctggacc cgccgcccca tggcccaggc gtctcgctca
181 ggtagcctgc ctccactcgt tatcgtgccc ccgctgaggg cgcaacccgg gggcactggg
241 gaggagcagt gggagagaag tcgaacgggc ggtcttcgct gggaggttca ctgctggccg
301 agcggaactt ctggagggac gccgtggtgg ccgacgccgg cggatgtgag cgaggactac
361 gaggctgatg ctgcggcctg gaggcggggg cccgcaggtg gcggcccgat ccctcccgcg
421 ctgcagcgtc tccgggcggt gttgctgcgg ctgcatcgcg agcgggagca gctcctccag
481 gcccgagact gcgcctacca cctacagtcg gctgtgcgac tcatgaagac cctgagtcct
541 ggctcgccat ccggcggccc tagccccttg ccccagtggt accgcgacct gcagctgcac
601 ccttcccaag gggcggttct gcgaatcggc cctggggaga ctctcgagcc gctgctgcta
661 gcgcgcccca tcggactagc cgcccagtgc ctggaggctg tcatcgagat gcagcttcgc
721 gctctcggcc gggagcccgc cagcccgggc ctgtcgtccc aactcgccga gctgctcttt
781 gcacttcccg cctaccacac actacagaga aaagccttga gccacgtccc aggggccgca
841 cgtcctttcc ccacgtcccg tgtgctccgc ctcttgacgg gggagcgggg ttgccaggtg
901 gcaagtcggc tggacgaggc gctccaagga tcggcgttga gggaccagct ccgcaggcgg
961 tgccaagagg agggggatct gctaccaggg ctgctgggcc tggtcggggg cgtggcgggt
1021 tcagccagct gtggactagg gctcggaggg gctggggcct tgtggtgcca atactggacc
1081 ctgctgtggg cagcctgtgc tcagagtctg gacctaaatc tgggaccctg gagggacccc
1141 agggcaacag cgcaacagct gagtcaggca ctgggtcagg catccctgcc tcaggagtgt
1201 gagaaggagc tggcatcttt gtgtcacaga ctacttcatc agtcgcttat ctggagctgg
1261 gaccaaggtt tctgccaggc cttgggatca gctcttgggg gtcagagcag ccttcccaca
1321 tcctctggca ctgctgaact tttgcagcag ctctttcctc ctctcttgga tgcccttcga
1381 gagcccaggt tacgacggat tttctgccag cctgcaggtc tctgtaccct tcagaccacc
1441 ttgctctggt tcctgggcag agctcagcag tacttggcag catgggaccc agcttccttc
1501 ctgctcctga tccaaaagga cttacctcct ctgttgcatg aggcagaagc tttgtatagc
1561 ctggcctcag aggaaagctt agctctggaa gtggagcagc agctgggcct ggagatccag
1621 aagctgactg cacagatcca gctcctgcct gaagagtcac taagtgtctt ttctcaagaa
1681 tgtcataaac aagccatgca aggtttcaag ctctacatgc cacggggtcg gtactggcgg
1741 cttcgtctct gtcctgaacc tcccagtgct cctagtgagt atgctggttt agtggtccgc
1801 accgtactgg agcctgtgtt gcaaggattg caagggttgc cacctcaagc ccaggcccct
1861 gcccttggtc aggctctgac ggccatcgtg ggtgcctggc ttgaccacat tcttacccat
1921 gggattcggt tcagcctgca gggagcgctg cagctcaaac aagactttgg agtggtcagg
1981 gagttgctgg aagaggagca gtggagcctg tcccctgatc tccgccagac cctgctcatg
2041 ctcagcatct tccagcagct ggatggggcc ctactgtgtc tgttgcagca gcccctgccc
2101 aagtctcaag tccacaggag gcccccctgt tgctgtgctt gtcaggaggt ccagaccacg
2161 aaattgccca gcagctgcct caatagcctg gagagcttgg agcccccgct ccagcctgga
2221 acatctccag cccagacagg tcagctgcaa agcacactag gaggaagggg acctagcccg
2281 gagggctacc tggtgggaaa tcagcaggcc tggcttgccc tcaggcaaca ccagcgaccc
2341 cgttggcacc tgccgttttt ttcctgcctg ggaaccagtc ctgaatccta aggagcctgg
2401 gaccaggagc cagaaagtta agagctcccc atctctagct ggaaaatcca gacatttgga
2461 attgcatatt aaggaagccc agaactggaa gcttggagga aagcatactg gagaataagc
2521 tacaaagagc ctgggcttaa gaatccaaga ttaggggctg ggcgaggtgg ctcacgcctg
2581 taatccagca ctttgggaga ccgaggcagg cggatcatga ggtcaggaga tcaagaccat
2641 cctggctaac acggtgaaac cctgtttcca ctaaaaatac aaaaaattag ccgggcgtgg
2701 tggcgggtgc ctgtagtccc agctactcgg gaggctgagg caggagaatg gtgtgaacct
2761 gggaggcgga gcttgcagtg agctgagatc cagccacagc actccagcct gggtgacaga
2821 gcaagactct gtctc
//