LOCUS AK075516 1959 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA PSEC0214 fis, clone HEMBA1004100.
ACCESSION AK075516
VERSION AK075516.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1959)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nishikawa,T., Suzuki,Y., Kawai-Hio,Y., Hayashi,K.,
Ishii,S., Saito,K., Yamamoto,J., Wakamatsu,A., Nagai,T.,
Nakamura,Y., Nagahari,K., Sugano,S. and Isogai,T.
TITLE HRI human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT HRI human cDNA sequencing project; cDNA 5'- & 3'-end one pass
sequencing, clone selection and full insert sequencing: Helix
Research Institute (supported by Japan Key Technology Center
etc.); cDNA library construction: Institute of Medical Science,
University of Tokyo, Laboratory of Genome Structure, Human Genome
Center.
FEATURES Location/Qualifiers
source 1..1959
/clone="HEMBA1004100"
/clone_lib="HEMBA1"
/db_xref="H-InvDB:HIT000082549"
/db_xref="taxon:9606"
/dev_stage="embryo, 10 weeks"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="whole embryo, mainly head"
CDS 56..481
/codon_start=1
/protein_id="BAC11665.1"
/translation="MFTSTGSSGLYKAPLSKSLLLVPSALSLLLALLLPHCQKLFVYD
LHAVKNDFQPGTCVCSVCTILLLHTKSPSGTNSGSVVHHKQDIDLYIGTAAFHLWFLH
LDCSHKWTYVRSVLRQQNVPGASGALHPQLDGKILFLDT"
BASE COUNT 448 a 509 c 509 g 493 t
ORIGIN
1 gcacttcagc ttcccctccc ccggcgccct ctggggctcc gagcccggcg ggaccatgtt
61 caccagcacc ggctccagtg ggctctacaa ggcgcctctg tcgaagagcc ttctgctggt
121 ccccagtgcc ctctccctcc tgctcgccct cctcctgcct cactgccaga agctctttgt
181 gtatgacctt cacgcagtca agaacgactt ccagcctggc acctgtgttt gctctgtttg
241 taccatttta ctgctccata ccaagagtcc aagtggcaca aattctgggt ccgttgtcca
301 tcacaaacaa gacattgatt tatatattgg gactgcagct tttcacctct ggttcctaca
361 tctggattgt agccataagt ggacttatgt ccggtctgtg ctacgacagc aaaatgttcc
421 aggtgcatca ggtgctctgc atccccagct ggatggcaaa attcttttct tggacacttg
481 aacccatctt ctcttcttca gaacccacca gcgaagccag aattgggatg ggagccacgc
541 tggacatcca gagacagcag agaatggagc tgctggaccg gcagctgatg ttctctcagt
601 ttgcacaagg gaggcgacag agacagcagc agggaggaat gatcaattgg aatcgtcttt
661 ttcctccttt acgtcagcga caaaacgtaa actatcaggg cggtcggcag tctgagccag
721 cagcgccccc tctagaagtt tctgaggaac aggtcgcccg gctcatggag atgggatttt
781 ccaagaggtg atgctttgga agccctgaga gcttcaaaca atgacctcaa tgtcgccacc
841 aacttcctgc tgcagcactg atagtcccag gccaacactg ggaccggacc ggcagccgag
901 tgacagtgcg tggtccccac catcagatca gcccggggac cgagcatctc tggtgctgat
961 gttcttgtgg gaagagggag gttccaccgc acccctgccc tcaaccgcaa gactgttgcc
1021 gttttagtgt ggagataagt ttgccattac attagcatgt attttctatc tatattttta
1081 ttgggcattt tccctaggtt ggagagtcag cactcgtttt gaatgtgttt aaaatgcatt
1141 aaaatggaag atttctgcag gcagttgaat ggcactccag atggggaatt gctgtaaccc
1201 tcttactgta acatgtcatc tcctgcgtcg tgatggggag agggtaatgt tacttcacaa
1261 aggacatgtc agatccttct tcatggactt ttttagttac tgttttttct ctcaaacttg
1321 ttttcgaatc tcctgggagt gagggagaaa cagggagctg aatcctcccc caagctgttc
1381 caggccagag gactctgcag taccttctcc tacatctagt aacaaagaat ggtgataacc
1441 atgcactggt tcaaggttct ggagttctcc atgaaacttg ggttaatttt gctcagagta
1501 tccagagtta gccactaggc tgcgggtgaa atgggatgga gaagaacaac agcgggcttc
1561 ctggagccac atgggctgac tagggcactc tgtggctggc ctggcatggg ctcagcccag
1621 gaagaggaga aacgatccct tgcctgcccc tccctgtggc agggctaact gcctggccct
1681 cctggctcgc agccagccag ccccctggca gcaggttctc ctcagggctt gggtcttcaa
1741 cctgtggcga caggaggcag ggcagactgt ggaggacagg atgcaggtca gggagaggga
1801 aggcaggggt ggaccgccat gagcatgaaa agacccgaag caagttgact cttgcaatgt
1861 gcaactgtta tgttctgcaa aatgagcaac gatgtatcaa attgatgcaa atttagatgt
1921 tgatacttac aataaagttt ttaatgtgtt ttactcttc
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