LOCUS AK075393 1976 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA PSEC0083 fis, clone NT2RP2005506, highly similar
to CATHEPSIN B PRECURSOR (EC 3.4.22.1).
ACCESSION AK075393
VERSION AK075393.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1976)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nishikawa,T., Suzuki,Y., Kawai-Hio,Y., Hayashi,K.,
Ishii,S., Saito,K., Yamamoto,J., Wakamatsu,A., Nagai,T.,
Nakamura,Y., Nagahari,K., Sugano,S. and Isogai,T.
TITLE HRI human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT HRI human cDNA sequencing project; cDNA 5'- & 3'-end one pass
sequencing, clone selection and full insert sequencing: Helix
Research Institute (supported by Japan Key Technology Center
etc.); cDNA library construction: Institute of Medical Science,
University of Tokyo, Laboratory of Genome Structure, Human Genome
Center.
FEATURES Location/Qualifiers
source 1..1976
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2005506"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000082428"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 152..1171
/codon_start=1
/protein_id="BAG52127.1"
/translation="MWQLWASLCCLLVLANARSRPSFHPLSDELVNYVNKRNTTWQAG
HNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQ
GSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWN
FWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPT
YKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMG
GHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQ
YWEKI"
BASE COUNT 463 a 539 c 542 g 432 t
ORIGIN
1 aaaaaaaaac ttagggccgg ggctggccca ggctacggcg gctgcagggc tccggcaacc
61 gctccggcaa cgccaaccgc tccgctgcgc gcaggctggg ctgcaggctc tcggctgcag
121 cgctgggtgg atctaggatc cggcttccaa catgtggcag ctctgggcct ccctctgctg
181 cctgctggtg ttggccaatg cccggagcag gccctctttc catcccctgt cggatgagct
241 ggtcaactat gtcaacaaac ggaataccac gtggcaggcc gggcacaact tctacaacgt
301 ggacatgagc tacttgaaga ggctatgtgg taccttcctg ggtgggccca agccacccca
361 gagagttatg tttaccgagg acctgaagct gcctgcaagc ttcgatgcac gggaacaatg
421 gccacagtgt cccaccatca aagagatcag agaccagggc tcctgtggct cctgctgggc
481 cttcggggct gtggaagcca tctctgaccg gatctgcatc cacaccaatg cgcacgtcag
541 cgtggaggtg tcggcggagg acctgctcac ctgctgtggc agcatgtgtg gggacggctg
601 taatggtggc tatcctgctg aagcttggaa cttctggaca agaaaaggcc tggtttctgg
661 tggcctctat gaatcccatg tagggtgcag accgtactcc atccctccct gtgagcacca
721 cgtcaacggc tcccggcccc catgcacggg ggagggagat acccccaagt gtagcaagat
781 ctgtgagcct ggctacagcc cgacctacaa acaggacaag cactacggat acaattccta
841 cagcgtctcc aatagcgaga aggacatcat ggccgagatc tacaaaaacg gccccgtgga
901 gggagctttc tctgtgtatt cggacttcct gctctacaag tcaggagtgt accaacacgt
961 caccggagag atgatgggtg gccatgccat ccgcatcctg ggctggggag tggagaatgg
1021 cacaccctac tggctggttg ccaactcctg gaacactgac tggggtgaca atggcttctt
1081 taaaatactc agaggacagg atcactgtgg aatcgaatca gaagtggtgg ctggaattcc
1141 acgcaccgat cagtactggg aaaagatcta atctgccgtg ggcctgtcgt gccagtcctg
1201 ggggcgagat gggggtagaa atgcatttta ttctttaagt tcacgtaaga tacaagtttc
1261 agacagggtc tgaaggactg gattggccaa acatcagacc tgtcttccaa ggagaccaag
1321 tcctggctac atcccagcct gtggttacag tgcagacagg ccatgtgagc caccgctgcc
1381 agcacagagc gtccttcccc ctgtagacta gtgccgtagg gagtacctgt tgccccagct
1441 gactgtggcc ccctccgtga tccatccatc tccagggagc aagacagaga cccaggaatg
1501 gaaagcggag ttcctaacag gatgaaagtt cccccatcag ttcccccagt acctccaagc
1561 aagtagcttt ccacatttgt cacagaaatc agaggagaga tggtgttggg agccctttgg
1621 agaacgccag tctcccaggc cccctgcatc tatcgagttt gcaatgtcac aacctctctg
1681 atcttgtgct cagcatgatt ctttaataga agttttattt tttcgtgcac tctgctaatc
1741 atgtgggtga gccagtggaa cagcgggaga cctgtgctag ttttacagat tgcctcctta
1801 tgacgcggct caaaaggaaa ccaagtggtc aggagttgtt tctgacccac tgatctctac
1861 taccacaagg agaatagttt aggagaaacc agcttttact gtttttgaaa aattacagct
1921 tcaccctgtc aagttaacaa ggaatgcctg tgccaataaa aggtttctcc aacttg
//