LOCUS AK075380 2510 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA PSEC0070 fis, clone NT2RP2001508, highly similar
to Source of immunodominant MHC-associated peptides.
ACCESSION AK075380
VERSION AK075380.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2510)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nishikawa,T., Suzuki,Y., Kawai-Hio,Y., Hayashi,K.,
Ishii,S., Saito,K., Yamamoto,J., Wakamatsu,A., Nagai,T.,
Nakamura,Y., Nagahari,K., Sugano,S. and Isogai,T.
TITLE HRI human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT HRI human cDNA sequencing project; cDNA 5'- & 3'-end one pass
sequencing, clone selection and full insert sequencing: Helix
Research Institute (supported by Japan Key Technology Center
etc.); cDNA library construction: Institute of Medical Science,
University of Tokyo, Laboratory of Genome Structure, Human Genome
Center.
FEATURES Location/Qualifiers
source 1..2510
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2001508"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000082415"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 94..954
/codon_start=1
/protein_id="BAC11581.1"
/translation="MLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDF
REAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNE
TAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQG
EFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEE
AFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFK
KGKKISKKTV"
BASE COUNT 802 a 412 c 489 g 807 t
ORIGIN
1 ttgtatgata aggcaggtaa agtgaggaaa catgcaactg aacaggaaaa aactgaagag
61 ggattaggcc ctaatataaa aagcattgtc accatgttga tgctgatgct attgatgatg
121 tttgctgtcc actgtacctg ggtcacaagc aatgcctact ctagtccaag tgtagtcctg
181 gcctcataca atcatgatgg caccaggaat atcttagatg attttagaga agcttacttt
241 tggctaaggc aaaatacaga tgaacatgca cgagtaatgt cttggtggga ttatggctat
301 cagatagctg gaatggctaa tagaactacg ttggtggata ataacacctg gaataacagc
361 cacatagcac tggtgggaaa agctatgtct tctaatgaaa cagcagccta taaaatcatg
421 aggactctag atgtagatta tgttttggtt atttttggag gggttattgg ctattctggt
481 gatgatatca acaaatttct ctggatggtt aggatagctg aaggagaaca tcccaaagac
541 attcgggaaa gtgactattt taccccacag ggagaattcc gtgtagacaa agcaggatcc
601 cctactttgt tgaattgcct tatgtataaa atgtcatact acagatttgg agaaatgcag
661 ctggattttc gtacaccccc aggttttgac cgaacacgta atgctgagat tggaaataag
721 gacattaaat tcaaacattt ggaagaagcc tttacatcag aacactggct tgttaggata
781 tataaagtaa aagcacctga taacagggag acattagatc acaaacctcg agtcaccaac
841 attttcccaa aacagaagta tttgtcaaag aagactacca aaaggaagcg tggctacatt
901 aaaaataagc tggtttttaa gaaaggcaag aaaatatcta agaagactgt ttaaatgcac
961 tgttctggtt cctaacttga agcagttgtc cttgtgagaa ccggtctttg cctttagctc
1021 atgtcgtgtt tcacagcaaa gagggtacag aaccatcact ggtccaggtt aatgtacaaa
1081 attttctggc aatgcctgat taaaaaaata aaattggctt gttgagaaca gctgttttcg
1141 atttctaatg tgaagcaaga cagagcactg ctgtaaatgt ctagcagcag attttttttt
1201 tattggtaca tattatcctt caaatctgag aatttggact aactgcacca aagaaccctc
1261 taatttggtc cctggcacat gcatacttgt caatgttttt attcttttac aagacctgca
1321 ttttatttga attacccgaa tagcaatatg taaaatacaa gtgacaaaat gtgatgagag
1381 cttcttgaac cggtaaacta gtacaggtct gagaaagaca tattagaaga atcattatac
1441 ttccttgaat tatatttatt ttcatgtttc tctaatgcaa agaatgtttc atcaaatgta
1501 tattttctgt tgcttactgt ttgctctggg aagaagctgc tgtttcaaag atggacctct
1561 gagtagctaa ttgattcaag tagttttttt atgttgacac attattactg ctgttagcag
1621 tcgttttcac caggtactta cagagcagat ttcatacatc attcattcaa gggctaaatt
1681 tatatttttt ggaaatcatg gcaactacac aggatgttgc ttaccaggac ggagttttgg
1741 tatcttagta ctgaagttag cactatgttt acatgcaaaa gattaaggaa aaaaccctta
1801 aagtggacag gtatccaaag ttcattttct gtgactcatc aaagtgacaa aagacttgta
1861 acaactttgc ctggactttt ttcattttac aacagttcat ccattcacaa tgattttgtt
1921 ctctgctcca tattttttaa tcccttaagc atttgatgaa acactcttta gtgctatatg
1981 cattttctta cttttgttaa aaatgtgaca attgtcaaaa aatgcactaa aatgtaaatg
2041 gagattgaac aagttcactt tccagcttat aggcaacttt atacagactt gaacattttc
2101 tccagttgtt tagtaaaagt gaaagagaaa gggtttttcc tgccacagga tataactttt
2161 ttttatataa caagcataac acaccactgc ttttggtgga aaagtgcaga atagtatgta
2221 ccttttatga agaaaaatgt aatttacaat attcagtgag aatgttactg ctgattttct
2281 tttccaaggt gtagaatatt ctttgattta tagaattcat ttttgaccca gatgatggtt
2341 cctttacaga acaataaaat ggctgaacat tttcacaaat agagtgtaac gaagtctgga
2401 tttctgatac cttgtcattt gggggatttt attttacttt gttgctttaa aattcaatgc
2461 agagaagttg ttgactgtag gggaaataaa gttaattcaa attttgtgtt
//