LOCUS AK075376 2682 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA PSEC0066 fis, clone NT2RP2001087, weakly similar
to TETRACYCLINE RESISTANCE PROTEIN.
ACCESSION AK075376
VERSION AK075376.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2682)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nishikawa,T., Suzuki,Y., Kawai-Hio,Y., Hayashi,K.,
Ishii,S., Saito,K., Yamamoto,J., Wakamatsu,A., Nagai,T.,
Nakamura,Y., Nagahari,K., Sugano,S. and Isogai,T.
TITLE HRI human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT HRI human cDNA sequencing project; cDNA 5'- & 3'-end one pass
sequencing, clone selection and full insert sequencing: Helix
Research Institute (supported by Japan Key Technology Center
etc.); cDNA library construction: Institute of Medical Science,
University of Tokyo, Laboratory of Genome Structure, Human Genome
Center.
FEATURES Location/Qualifiers
source 1..2682
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2001087"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000082411"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 45..1469
/codon_start=1
/protein_id="BAC11577.1"
/translation="MELGGHWDMNSAPRLVSETAERKQEQKTGTEAEAADSGAVGARR
FLLCLYLVGFLDLFGVSMVVPLLSLHVKSLGASPTVAGIVGSSYGILQLFSSTLVGCW
SDVVGRRSSLLACILLSALGYLLLGAATNVFLFVLARVPAGIFKHTLSISRALLSDVV
PEKERPLVIGHFNTASGVGFILGPVVGGYPTELEDGFYLTAFICFLVFILNAGLVWFF
PWREAKPGSTEKGLPLRKTHVLLGRSHDTVQEAATSRRARASKKTAQPWVEVVLALRN
MRNLLFSEMWDIFLVRLLMAMAVMLYYSNFVLALEERFGVRPKVTGYLISYSSMLGAV
AGLALGPILRLYKHNSQALLLHSSILTCTLLLLYSLAPTMGAVVLSSTLLSFSTAIGR
TCITDLQLTVGGAQASGTLIGVGQSVTAVGRIIAPLLSGVAQEVSPCGPPSLGAVLAL
VAIFIMSLNKRHSSGDGNSKLKSE"
BASE COUNT 597 a 667 c 748 g 670 t
ORIGIN
1 aggtccgcgg ctgctgctgc gtccgacccc cggccggcgc gggtatggag cttgggggtc
61 actgggacat gaactcggcc ccgaggctgg tctcggagac cgcagagcgg aaacaggagc
121 agaagacagg aaccgaggcg gaggctgccg actccggtgc cgtcggagcc cgccgcttcc
181 tgctctgtct ctacttggtg ggcttcttgg atttgtttgg tgtcagcatg gttgtgcctt
241 tattgagcct tcatgtcaag tcccttggag caagtccaac agttgctgga atagtaggct
301 cctcctatgg cattttgcaa ctcttttcta gcacattggt gggctgctgg agcgatgtag
361 tgggaagacg gtcttccttg ctggcatgca ttctactcag tgctctgggc tatctccttc
421 tcggagcagc caccaatgtg tttctgtttg tcctggctag agtcccggca ggtattttta
481 aacacactct ctccatctca agggctctac tttctgatgt ggttccagag aaggaacggc
541 cgcttgtaat cggacacttc aacacagcct ccggtgtggg cttcatcttg ggccccgtgg
601 tcggtggcta tcccactgaa ttagaggatg ggttttatct cacagccttc atctgctttt
661 tggtcttcat tctcaatgct ggtctcgttt ggttctttcc atggagggaa gcaaaaccgg
721 gcagtacaga gaagggcctg ccattgcgaa agacccatgt gctgttggga aggagccatg
781 acacagtgca ggaggcagcc accagccgca gagccagggc cagcaagaag actgcccagc
841 cctgggtcga agtagtgttg gccttgcgga acatgaggaa cctgctgttt tccgaaatgt
901 gggacatatt tctggtgcgc ttgctgatgg ccatggcagt catgctgtac tacagtaact
961 ttgtcctggc cctggaggag cgctttgggg tgcggcccaa ggtgacaggc tacctcatca
1021 gttacagcag catgctgggg gccgtggccg gccttgccct ggggccaatc ctacggctgt
1081 acaagcacaa ctcgcaggca ctgctgctgc attccagcat actcacctgc acactgctgc
1141 tgctctactc cttggccccc accatgggtg cagttgtcct ctcctccact ctcctgtcct
1201 tctccactgc cattggcagg acgtgcatca cggacctcca gctgactgtg ggcggggccc
1261 aggccagcgg cacccttatt ggcgtggggc agtctgtgac tgcagtgggc cgcatcatcg
1321 cccctctcct ctcgggggtt gcccaggagg tcagcccttg cggccccccc agcctgggtg
1381 ctgtgttagc cttagtggcc attttcataa tgtctctaaa caagcgacac tctagtggtg
1441 atgggaatag taaattaaaa agtgagtaga tggatttgga caacataaag caacaaaatt
1501 tgagatggtt gaatgagggc cggaggccat gatgaaaagg gcactttgga aagggttggg
1561 gtggaaggga aatatttccg ggtgggtgtg agctgttggg cttccaggtc agctcttggc
1621 catgcagcca tgcctgcagg atgatcagaa gtcacggcac ctcatgggaa ggttaagact
1681 ggagcaaagc ttttccaagg tgagcatatt cagcgtttac ctggaagtct cttcttccca
1741 cctgggctaa tcaggttaca attttcaagg gtaaacaaac taccagctac aggataggga
1801 agtggtggtg gaataaaaga acatgatacc tggaggaagg gaagaaacca caagcatttt
1861 cctactgaaa aatagggtga catgtcagtc aaattctgat caactggaac ttgagtttcc
1921 agttaaattc catacactag gagggagttt tctatcaaaa tcctgcagat tgaagaagct
1981 ggtttattag aaccagcctg tcgcttttca aagctggtta aaaataagat actgacctca
2041 cccctagaga tgattcagtg ggcctgggtg gggccaagaa atccgtggtg ttttgtaagc
2101 actgctggcg atttctcatg gcagtaaaga ccacactgag actgcactga ttttgattaa
2161 agtacatagg cttggctgtt tttacacagc taactaaatt tggactctta aaattgtgtt
2221 tgtaatcaca gtgtgttaca cctttcccag ctctcagcag ttgtactgca tgatggagca
2281 gcgggcccct gggacctgtt ccctgtgctc cctgcctctg agagcccagc ttgctctgaa
2341 gttggactaa accacttaga tgctggggtt gccacagcct gctcctggaa gcctgcattc
2401 cagtcaggtt gcatccttct ggtgctgaca gaacccagtg gtgaagccaa agcacaggaa
2461 tgctttaaaa atgaacagtt tatgcagaat aagggtcagg agtcatgcca gaccagaact
2521 tgatgctact gcgtcttgtg ttaagaatca tttcctggcc aggctcggtg gctcaggcct
2581 gtaatcccag cactttggga ggctgaggca ggtgaatcac ttggtcagga gatggagacc
2641 atcctggcta acatggtgaa accccgtctc tactaaaaat ac
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