LOCUS AK075372 1948 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA PSEC0060 fis, clone NT2RP2000638, highly similar
to Secreted frizzled-related protein 2 precursor.
ACCESSION AK075372
VERSION AK075372.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1948)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nishikawa,T., Suzuki,Y., Kawai-Hio,Y., Hayashi,K.,
Ishii,S., Saito,K., Yamamoto,J., Wakamatsu,A., Nagai,T.,
Nakamura,Y., Nagahari,K., Sugano,S. and Isogai,T.
TITLE HRI human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT HRI human cDNA sequencing project; cDNA 5'- & 3'-end one pass
sequencing, clone selection and full insert sequencing: Helix
Research Institute (supported by Japan Key Technology Center
etc.); cDNA library construction: Institute of Medical Science,
University of Tokyo, Laboratory of Genome Structure, Human Genome
Center.
FEATURES Location/Qualifiers
source 1..1948
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2000638"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000082407"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 235..1122
/codon_start=1
/note="highly similar to Secreted frizzled-related
protein 2 precursor"
/protein_id="BAG52124.1"
/translation="MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIP
ANLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPV
CLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDH
LLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKS
KTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRW
QKGQREFKRISRSIRKLQC"
BASE COUNT 464 a 567 c 445 g 472 t
ORIGIN
1 tcgttctgct cccccgggtc ggagcccccc ggagctgcgc gcgggcttgc agcgcctcgc
61 ccgcgctgtc ctcccggtgt cccgcttctc cgcgccccag ccgccggctg ccagcttttc
121 ggggccccga gtcgcaccca gcgaagagag cgggcccggg acaagctcga actccggccg
181 cctcgccctt ccccggctcc gctccctctg ccccctcggg gtcgcgcgcc cacgatgctg
241 cagggccctg gctcgctgct gctgctcttc ctcgcctcgc actgctgcct gggctcggcg
301 cgcgggctct tcctctttgg ccagcccgac ttctcctaca agcgcagcaa ttgcaagccc
361 atcccggcca acctgcagct gtgccacggc atcgaatacc agaacatgcg gctgcccaac
421 ctgctgggcc acgagaccat gaaggaggtg ctggagcagg ccggcgcttg gatcccgctg
481 gtcatgaagc agtgccaccc ggacaccaag aagttcctgt gctcgctctt cgcccccgtc
541 tgcctcgatg acctagacga gaccatccag ccatgccact cgctctgcgt gcaggtgaag
601 gaccgctgcg ccccggtcat gtccgccttc ggcttcccct ggcccgacat gcttgagtgc
661 gaccgtttcc cccaggacaa cgacctttgc atccccctcg ctagcagcga ccacctcctg
721 ccagccaccg aggaagctcc aaaggtatgt gaagcctgca aaaataaaaa tgatgatgac
781 aacgacataa tggaaacgct ttgtaaaaat gattttgcac tgaaaataaa agtgaaggag
841 ataacctaca tcaaccgaga taccaaaatc atcctggaga ccaagagcaa gaccatttac
901 aagctgaacg gtgtgtccga aagggacctg aagaaatcgg tgctgtggct caaagacagc
961 ttgcagtgca cctgtgagga gatgaacgac atcaacgcgc cctatctggt catgggacag
1021 aaacagggtg gggagctggt gatcacctcg gtgaagcggt ggcagaaggg gcagagagag
1081 ttcaagcgca tctcccgcag catccgcaag ctgcagtgct agtcccggca tcctgatggc
1141 tccgacaggc ctgctccaga gcacggctga ccatttctgc tccgggatct cagctcccgt
1201 tccccaagca cactcctagc tgctccagtc tcagcctggg cagcttcccc ctgccttttg
1261 cacgtttgca tccccagcat ttcctgagtt ataaggccac aggagtggat agctgttttc
1321 acctaaagga aaagcccacc cgaatcttgt agaaatattc aaactaataa aatcatgaat
1381 atttttatga agtttaaaaa tagctcactt taaagctagt tttgaatagg tgcaactgtg
1441 acttgggtct ggttggttgt tgtttgttgt tttgagtcag ctgattttca cttcccactg
1501 aggttgtcat aacatgcaaa ttgcttcaat tttctctgtg gcccaaactt gtgggtcaca
1561 aaccctgttg agataaagct ggctgttatc tcaacatctt catcagctcc agactgagac
1621 tcagtgtcta agtcttacaa caattcatca ttttatacct tcaatgggaa cttaaactgt
1681 tacatgtatc acattccagc tacaatactt ccatttatta gaagcacatt aaccatttct
1741 atagcatgat ttcttcaagt aaaaggcaaa agatataaat tttataattt cttacttaac
1801 ttttgcaaat taaacccatt gtagcttacc tgtaatatac atagtagttt acctttaaaa
1861 gttgtaaaaa tattgcttta accaacactg taaatatttc agataaacat tatattcttg
1921 tatataaact ttacatcctg ttttacct
//