LOCUS AK075342 1851 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA PSEC0021 fis, clone NT2RP1000279.
ACCESSION AK075342
VERSION AK075342.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1851)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (20-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Nishikawa,T., Suzuki,Y., Kawai-Hio,Y., Hayashi,K.,
Ishii,S., Saito,K., Yamamoto,J., Wakamatsu,A., Nagai,T.,
Nakamura,Y., Nagahari,K., Sugano,S. and Isogai,T.
TITLE HRI human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT HRI human cDNA sequencing project; cDNA 5'- & 3'-end one pass
sequencing, clone selection and full insert sequencing: Helix
Research Institute (supported by Japan Key Technology Center
etc.); cDNA library construction: Institute of Medical Science,
University of Tokyo, Laboratory of Genome Structure, Human Genome
Center.
FEATURES Location/Qualifiers
source 1..1851
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP1000279"
/clone_lib="NT2RP1"
/db_xref="H-InvDB:HIT000082377"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pUC19FL3"
/note="mRNA from NT2 neuronal precursor cells after
48-hours retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 171..521
/codon_start=1
/protein_id="BAG52117.1"
/translation="MKGLRSLAATTLALFLVFVFLGNSSCAPQRLLERRNWTPQAMLY
LKGAQGRRFISDQSRRKDLSDRPLPERRSPNPQLLTIPEAATILLASLQKSPEDEEKN
FDQTRFLEDSLLNW"
BASE COUNT 570 a 361 c 344 g 576 t
ORIGIN
1 aaataatctt gcttgaagac tgacaagatg tccctgtgga ctcccaaact ctactccaga
61 tggggaggtg cccttaacac caagatttta aaagctccaa tttcagagca agagtcgaaa
121 actcacagat aaagttatag ttatttcagg gttctgaaaa gacgcagaac atgaagggac
181 tcagaagtct ggcagcaaca accttggctc ttttcctggt gtttgttttc ctgggaaact
241 ccagctgcgc tccgcagaga ctgttggaga gaaggaactg gactcctcaa gctatgctct
301 acctgaaagg ggcacagggt cgccgcttca tctccgacca gagccggaga aaggacctct
361 ccgaccggcc actgccggaa agacgaagcc caaatcccca actactaact attccggagg
421 cagcaaccat cttactggcg tcccttcaga aatcaccaga agatgaagaa aaaaactttg
481 atcaaaccag attcctggaa gacagtctgc ttaactggtg aaaatatact ggattatgtt
541 taattatggt tctattctct ttgaaaacat gaaccatgtg aataaaacct ttggaccctt
601 ttattccatt tgtaatctta agaacacaca cagatagttt tattctttca gaaacaaaat
661 atatatagga tgcttagctg agaacatcat cttctttcat tgcttcaggt cctgtttaga
721 tgaccaaaaa tgttttcaga tcaccttgtg tcttactctt gagtttctta gaatatttat
781 aattataagg ctgaagacta aagtgttctt tccttttaac tatagccagt acctgtcttg
841 atcttagttg tgtttttttt tttcattttg ttacccactt gcattttgtt ttcactcagc
901 agaaattctc cttctctgtt ttccttttat cccatcccca agaatgtgga aggaaggtga
961 gaaacatggc aggatgggaa ataggagagt atgactctct atagctcatc caggagtaat
1021 caattaagaa gataaattgg atgactgtgg agaagctctg tgataggaac acttcagtgt
1081 ggttgctgag aggagacagt cattgaggta gaaggtttgc caaagatcca gagctcagag
1141 ctccctttgt gctctttggg aattaccttg cattcagttt agaaacatgg atctaaaagt
1201 tactgggaaa taagcagatg gagacacact ctgttgttta cgtattggaa gaagggaaca
1261 agccagtttt gttagaggta actcattttc catgaccaaa cagactcaac agattcaagt
1321 actctgctta ctctaattga ctagactcta ggttttattt gacatcatag cattacataa
1381 atcactctga taacataagt gcacagtaat atgcctgatc tcttcctttt taaaagccaa
1441 cttgagttca gtaccatctg aatacacaca catgcacata tacccacaca cgcatacaca
1501 catactcctg tggcaaacat aataatgtat ttatttagaa ttataatatg accatcatgt
1561 taattatttt ttacctaatc agagttgtta ttgacaaatg tcataagtgg aaagtattaa
1621 ttcttattgt catcagtatt tagccattat ttagtagctc aagaatatct ttatgtgaat
1681 gtctctgtaa cttggaattg caatttcact gtgttaagta atcagaactc tgcttataag
1741 atttatctgt atcttgtttc ataatttaat aatgaaacta aattcaagtt aatgtaatgt
1801 tgatctccgt cgaaaaataa cttgtgagca ttaaaatatc tgtatggcat t
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