LOCUS AK075010 2680 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90529 fis, clone NT2RP4001879.
ACCESSION AK075010
VERSION AK075010.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2680)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..2680
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP4001879"
/clone_lib="NT2RP4"
/db_xref="H-InvDB:HIT000082045"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 106..1308
/codon_start=1
/protein_id="BAC11350.1"
/translation="MAANYSSTSTRREHVKVKTSSQPGFLERLSETSGGMFVGLMAFL
LSFYLIFTNEGRALKTATSLAEGLSLVVSPDSIHSVAPENEGRLVHIIGALRTSKLLS
DPNYGVHLPAVKLRRHVEMYQWVETEESREYTEDGQVKKETRYSYNTEWRSEIINSKN
FDREIGHKNPSAMAVESFMATAPFVQIGRFFLSSGPIDKVDNFKSLSLSKLEDPHVDI
IRRGDFFYHSENPKYPEVGDLRVSFSYAGLSGDDPDLGPAHVVTVIARQRGDQLVPFS
TKSGDTLLLLHHGDFSAEEVFHRELRSNSMKTWGLRAAGWMAMFMGLNLMTRILYTLV
DWFPVFRDLVNIGLKAFAFCVATSLTLLTVAAGWLFYRPLWALLIAGLALVPILVART
RVPAKKLE"
BASE COUNT 628 a 721 c 681 g 650 t
ORIGIN
1 tttcgaagct ggggctggca agaggccgct ggacaccacg ctccagtcgt cagcccactt
61 cctagctgaa cagcgcgagg cggcggcagc gagccgggtc ccaccatggc cgcgaattat
121 tccagtacca gtacccggag agaacatgtc aaagttaaaa ccagctccca gccaggcttc
181 ctggaacggc tgagcgagac ctcgggtggg atgtttgtgg ggctcatggc cttcctgctc
241 tccttctacc taattttcac caatgagggc cgcgcattga agacggcaac ctcattggct
301 gaggggctct cgcttgtggt gtctcccgac agcatccaca gtgtggctcc ggagaatgaa
361 ggaaggctgg tgcacatcat tggcgcctta cggacatcca agcttttgtc tgatccaaac
421 tatggggtcc atcttccggc tgtgaaactg cggaggcacg tggagatgta ccaatgggta
481 gaaactgagg agtccaggga gtacaccgag gatgggcagg tgaagaagga gacgaggtat
541 tcctacaaca ctgaatggag gtcagaaatc atcaacagca aaaacttcga ccgagagatt
601 ggccacaaaa accccagtgc catggcagtg gagtcattca tggcaacagc cccctttgtc
661 caaattggca ggtttttcct ctcgtcaggc cccatcgaca aagtcgacaa cttcaagtcc
721 ctgagcctat ccaagctgga ggaccctcat gtggacatca ttcgccgtgg agactttttc
781 taccacagcg aaaatcccaa gtatccagag gtgggagact tgcgtgtctc cttttcctat
841 gctggactga gcggcgatga ccctgacctg ggcccagctc acgtggtcac tgtgattgcc
901 cggcagcggg gtgaccagct agtcccattc tccaccaagt ctggggatac cttactgctc
961 ctgcaccacg gggacttctc agcagaggag gtgtttcata gagaactaag gagcaactcc
1021 atgaagacct ggggcctgcg ggcagctggc tggatggcca tgttcatggg cctcaacctt
1081 atgacacgga tcctctacac cttggtggac tggtttcctg ttttccgaga cctggtcaac
1141 attggcctga aagcctttgc cttctgtgtg gccacctcgc tgaccctgct gaccgtggcg
1201 gctggctggc tcttctaccg acccctgtgg gccctcctca ttgccggcct ggcccttgtg
1261 cccatccttg ttgctcggac acgggtgcca gccaaaaagt tggagtgaaa agaccctggc
1321 acccgcccga cacctgcgtg agccctagga tccaggtcct ctctcacctc tgacccagct
1381 ccatgccaga gcaggagccc cggtcaattt tggactctgc actccctctc ctcttcaggg
1441 gccagacttg gcagcatgtg caccaggttg gtgttcacca gctcatgtct tccccacatc
1501 tcttcttgcc agtaagcagc tttggtgggc agcagcagct catgaatggc aagctgacag
1561 cttctcctgc tgtttccttc ctctcttgga ctgagtgggt acggccagcc actcagccca
1621 ttggcagctg acaacgcaga cacgctctac ggaggcctgc tgataaaggg ctcagccttg
1681 ccgtgtgctg cttctcatca ctgcacacaa gtgccatgct ttgccaccac caccaagcac
1741 atctgtgatc ctgaagggcg gccgttagtc attactgctg agtcctgggt caccagcaga
1801 cacactgggc atggacccct caaagcaggc acacccaaaa cacaagtctg tggctagaac
1861 ctgatgtggt gtttaaaaga gaagaaacac tgaagatgtc ctgaggagaa aagctggaca
1921 tatactgggc ttcacactta tcttatggct tggcagaatc tttgtagtgt gtgggatctc
1981 tgaaggccct atttaagttt ttcttcgtta ctttgctgct tcatgtgtac tttcctaccc
2041 caagaggaag ttttctgaaa taagatttaa aaacaaaaca aaaaaaacac ttaatatttc
2101 agactgttac aggaaacacc ctttagtctg tcagttgaat tcagagcact gaaaggtgtt
2161 aaattggggt atgtggtttg attgataaaa agttacctct cagtattttg tgtcactgag
2221 aagctttaca atggatgctt ttgaaacaag tatcagcaaa aggatttgtt ttcactctgg
2281 gaggagaggg tggagaaagc acttgctttc atcctctggc atcggaaact cccctatgca
2341 cttgaagatg gtttaaaaga ttaaagaaac gattaagaga aaaggttgga agctttatac
2401 taaatgggct ccttcatggt gacgccccgt caaccacaat caagaactga ggcctgaggc
2461 tggttgtaca atgcccacgc ctgcctggct gctttcacct gggagtgctt tcgatgtggg
2521 cacctgggct tcctagggct gcttctgagt ggttctttca cgtgttgtgt ccatagcttt
2581 agtcttccta aataagatcc acccacacct aagtcacaga atttctaagt tccccaacta
2641 ctctcacacc cttttaaaga taaagtatgt tgtaaccagg
//