LOCUS AK075002 3462 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90521 fis, clone NT2RP3004647, weakly similar
to PUTATIVE MITOCHONDRIAL CARRIER YMR166C.
ACCESSION AK075002
VERSION AK075002.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3462)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..3462
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP3004647"
/clone_lib="NT2RP3"
/db_xref="H-InvDB:HIT000082037"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 150..1094
/codon_start=1
/protein_id="BAC11347.1"
/translation="MEDKRNIQIIEWEHLDKKKFYVFGVAMTMMIRVSVYPFTLIRTR
LQVQKGKSLYHGTFDAFIKILRADGITGLYRGFLVNTFTLISGQCYVTTYELTRKFVA
DYSQSNTVKSLVAGGSASLVAQSITVPIDVVSQHLMMQRKGEKMGRFQVRGNPEGQGV
VAFGQTKDIIRQILQADGLRGFYRGYVASLLTYIPNSAVWWPFYHFYAEQLSYLCPKE
CPHIVFQAVSGPLAAATASILTNPMDVIRTRVQVEGKNSIILTFRQLMAEEGPWGLMK
GLSARIISATPSTIVIVVGYESLKKLSLRPELVDSRHW"
BASE COUNT 789 a 933 c 851 g 889 t
ORIGIN
1 agcccagtcg agatggagga agatgggacc ggcagacggc attcgctggg aacgacggat
61 agactggggg ctgcggccta gaggtccggg cttggagttc gcctcagacg cggtggagcc
121 gccggagtct gagaaggtct tcaggcacca tggaggacaa acgcaacatc cagatcatcg
181 agtgggaaca cctggacaag aagaagttct acgtgtttgg tgtggcaatg acaatgatga
241 tccgtgtcag tgtctaccca ttcaccctca tccgcacccg gttgcaagtt cagaagggga
301 agagcctcta ccatgggacc ttcgatgcct tcatcaagat cctgcgagca gatggtatca
361 ctggcctcta ccgagggttc ctggtcaata ccttcaccct catctctggc cagtgttatg
421 tcaccactta tgagctcacc cggaagtttg tagctgacta cagccagagt aacacagtca
481 aatcactggt ggctggtggc tcagcctccc ttgtggccca gagcatcaca gtgcccattg
541 atgtagtctc ccagcacctg atgatgcaac gcaagggtga gaaaatgggc cgctttcagg
601 tgcgggggaa cccagaggga caaggggtag ttgcctttgg ccaaaccaag gacatcatca
661 ggcagatcct gcaggctgat ggacttcgcg gcttctatcg aggctatgtg gcttcactgc
721 ttacctatat cccaaacagt gctgtctggt ggcccttcta tcacttctat gcagagcagc
781 tctcctacct gtgtcctaag gagtgccctc acattgtctt tcaagctgtc tcggggcccc
841 tggctgcagc cactgcctcc atcctcacca atcccatgga tgtcatacga acccgtgtgc
901 aggttgaggg caagaactcc atcatcctga ccttcagaca gctgatggca gaagaagggc
961 cttggggcct catgaagggc ctctcggcca gaatcatctc agccacacct tccaccattg
1021 tcattgtggt gggctatgag agcctcaaga aactcagcct ccgacctgag ctggtggact
1081 cgagacactg gtaaccagtg gtggggagag aagcctgctg ttttccacac taccgtgggt
1141 caggggcaga gtggagagga cagcaccctc tccaggtgct cccaccacac acccagccct
1201 gccctgggcc aagtggccta tctgggatag ggatagagac tttgaactgc tcttgctgaa
1261 gaggctccac gcctggatcc cttgccccca ctatttaaaa ttctcttctg agctgggctc
1321 cctcactcag tccctgtatt tgatactggc ctaaagaccc caccccccac cctgccagcc
1381 cttcttctgg cttccccttc catctgtgtc cctgagaccc tgagaagagc tgtacataga
1441 gcttgcttac taccactggt tcttcctctt gggctttcag cccagactcc aagcagctgc
1501 tatcaaccct ctctcccttc atctcttagc cttgcttatt tttattttgg gaccgagctg
1561 cccactagat gactctgctt ttccctgcat ttggggctaa ggtgccaggt acttatttgc
1621 acagggagca ggagcagcaa aaaatctctg gttctccaga gcactcgtcc tctctttgga
1681 ggggttatta ggttgggaga aatgttgata cttttgtttt gtgtgtgtgt gtgtgtgtgt
1741 gtgtgtgtgt gtgtgtgtgt gtgtgtgttt taacatctgt gaaccaggct attagtcctg
1801 ctaaagcgcc aatcctgctg tcagagctca cccccttcct aagacaggta gaaaaatgta
1861 atgtagcttt ttccacaagc cacttccctg tcccttcagt ctcaggagcc ctaggagagt
1921 ctaagctggg gcatcccctg gcccagagga ctcccgtggt gggcacagtt ctaagtggat
1981 caggctgtct tgggtgcact ggacttggag cactaccttg agaagtcagg ttgagaaagt
2041 agttgatcta gaaggcaaca agtgggcatg tgttccccag cacattaccc aggccagcag
2101 agccaaacct aggagagggc agtgggtaga ttctctgccc caggcagcca tgacatacac
2161 ataaataccc caatcactca gacttacggc aacaagtgtt gtctcactat ggtgatctct
2221 aagatccaca tcactggatg cgtagtcatc ccagtcatgg taccctgtgg aggaatgctg
2281 gaagaacata aagagcagtt cagaaagtca cccaatacca ggaccactgc atttaccagc
2341 ctgatactgc caagattatc tgatgctctc ctcaggagct aggagaggag tgctccttcc
2401 tccctaccgc tactctcccc aagcctgtgt tgcaggtaga gaggtgcagc aaatagagaa
2461 ggcatgtcaa accctgcatt tctacctgag acgtgtgacc tggatgatcc tccaaaccct
2521 attggtccca ccccctggga aaggccatgg tgccagtttg aaaggtgcta gctacctgaa
2581 gccttgatat ttcttcatgg ctgccgcaca ttcttccacc ttggccagaa caggttctga
2641 aaaccacttc tctaccttca ccaccaccac tgcccatctt gatctctttg agggttttcc
2701 catttcactt gatcttattt ttgtttatcc cttcctgcac tttgtcaaga gagtcctcca
2761 gtttctatcc aggaatgttc acatccaaag ggttggaccc acggatcatt ctgaatcttc
2821 ctgcccctcc tcactgctta accctgagaa ccacaaatat aatggaagca gttcccccca
2881 ccctcacccc atctctttaa gctcatccta gcaagacctc tagagaccct agagactcga
2941 ctttagtcct tccccgccat ggcacagtgg ggaaggtgtc aatggggagt gtcacggaca
3001 ggaggtagga tcctgccgct cgcgtcttag tgtttctccc tcaagacttt ccttctgttt
3061 tgttgtcttg tgcagtattt tacagcccct cttgtgtttt tctttatttc tcgtacacac
3121 acgcagtttt aagggtgatg tgtgtataat taaaaggacc cttggcccat actttcctaa
3181 ttctttaggg actgggattg ggtttgactg aaatatgttt tggtggggat gggacggtgg
3241 acttccattc tccctaaact ggagttttgg tcggtaatca aaactaaaag aaacctctgg
3301 gagactggaa acctgattgg agcactgagg aacaagggaa tgaaaaggca gactctctga
3361 acgtttgatg aaatggactc ttgtgaaaat taacagtgaa tattcactgt tgcactgtac
3421 gaagtctctg aaatgtaatt aaaagttttt attgagcccc cg
//